Reviewed, UniProtKB/Swiss-Prot Q75W16 (AROG_ORYSJ)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase 2
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2
    DAHP synthase 2

Gene names

Name:	DAHPS2
Ordered Locus Names:	Os07g0622200, LOC_Os07g42960
ORF Names:	P0594D10.136

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the class-II DAHP synthase family.
Sequence caution	The sequence BAC10194.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 54	54	Chloroplast Potential
	Chain	55 – 539	485	Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic		PRO_0000247311
Regions
	Compositional bias	518 – 525	8	Poly-Arg

Sequences

	Sequence		Length	Mass (Da)
	Q75W16-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 69AE271D7CF17676	FASTA	539	59,334
	10 20 30 40 50 60 MALATNSAAV SGGAAAAASS APQPRLAATF LPMRRRTVSA VHAADPAKSN GPVQAAAKAS 70 80 90 100 110 120 SPSTVAAPEK KPVGLGKWTV DSWKAKKALQ LPEYPSQEEL DSVLKTIETF PPVVFAGEAR 130 140 150 160 170 180 HLEERLADAA MGRAFVLQGG DCAESFKEFN ANNIRDTFRI LLQMGAVLMF GGQMPVVKVV 190 200 210 220 230 240 GRMAGQFAKP RSDSFEERDG VKLPSYRGDN INGDTFDEKS RVPDPQRMIR AYAQSVATLN 250 260 270 280 290 300 LLRAFATGGY AAMQRVTQWN LDFMDHSEQG DRRYRELAHR VDEALGFMTA AGLTVDHPIM 310 320 330 340 350 360 TTTDFWTSHE CLLLPYEQSL TREDSTSGLF YDCSAHMLWV GERTRQLDGA HVEFLRGVAN 370 380 390 400 410 420 PLGIKVSDKM NPRDLVKLIE ILNPSNKPGR ITIITRMGAE NMRVKLPHLI RAVRNSGQIV 430 440 450 460 470 480 TWITDPMHGN TIKAPCGLKT RPFDSILAEV RAFFDVHDQE GSHPGGIHLE MTGQNVTECI 490 500 510 520 530 GGSRTVTFDD LSDRYHTHCD PRLNASQSLE LAFIIAERLR RRRMRSGVNS NLPLPPLAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cDNA encoding 3-deoxy-D-arabino heptulosonate-7-phosphate synthase expressed in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fujiminori.
[2]	"The gene encoding 3-deoxy-D-arabino heptulosonate-7-phosphate synthase expressed in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Fujiminori.
[3]	"Oryza sativa nipponbare chromosome 7 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB122058 mRNA. Translation: BAD15283.1. AB122082 Genomic DNA. Translation: BAD14926.1. AP004380 Genomic DNA. Translation: BAC10194.1. Sequence problems.
RefSeq	NP_001060314.1.
UniGene	Os.22631
3D structure databases
SMR	Q75W16. Positions 76-520.
ModBase	Search...
Genome annotation databases
GeneID	4343946.
KEGG	osa:4343946.
Organism-specific databases
Gramene	Q75W16.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROG_ORYSJ

Accession

Primary (citable) accession number: Q75W16
Secondary accession number(s): Q8LH96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)