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Protein

Anoctamin-5

Gene

Ano5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Does not exhibit calcium-activated chloride channel (CaCC) activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.17.1.2. the calcium-dependent chloride channel (ca-clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-5
Alternative name(s):
Gnathodiaphyseal dysplasia 1 protein homolog
Transmembrane protein 16E
Gene namesi
Name:Ano5
Synonyms:Gdd1, Tmem16e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:3576659. Ano5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 290290CytoplasmicSequence analysisAdd
BLAST
Transmembranei291 – 31121HelicalSequence analysisAdd
BLAST
Topological domaini312 – 37160ExtracellularSequence analysisAdd
BLAST
Transmembranei372 – 39221HelicalSequence analysisAdd
BLAST
Topological domaini393 – 45361CytoplasmicSequence analysisAdd
BLAST
Transmembranei454 – 47421HelicalSequence analysisAdd
BLAST
Topological domaini475 – 50228ExtracellularSequence analysisAdd
BLAST
Transmembranei503 – 52321HelicalSequence analysisAdd
BLAST
Topological domaini524 – 54825CytoplasmicSequence analysisAdd
BLAST
Transmembranei549 – 56921HelicalSequence analysisAdd
BLAST
Topological domaini570 – 66798ExtracellularSequence analysisAdd
BLAST
Transmembranei668 – 68821HelicalSequence analysisAdd
BLAST
Topological domaini689 – 72335CytoplasmicSequence analysisAdd
BLAST
Transmembranei724 – 74421HelicalSequence analysisAdd
BLAST
Topological domaini745 – 82581ExtracellularSequence analysisAdd
BLAST
Transmembranei826 – 84621HelicalSequence analysisAdd
BLAST
Topological domaini847 – 90458CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 904904Anoctamin-5PRO_0000191756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence analysis
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence analysis
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence analysis
Glycosylationi759 – 7591N-linked (GlcNAc...)Sequence analysis
Glycosylationi769 – 7691N-linked (GlcNAc...)Sequence analysis
Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ75UR0.
PRIDEiQ75UR0.

PTM databases

PhosphoSiteiQ75UR0.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, bone tissues and thyroid gland.4 Publications

Developmental stagei

In the developing embryo, expressed mainly in differentiating and developing somites and is associated with myotomal somite lineages.1 Publication

Inductioni

Up-regulated during the course of myogenic differentiation. Down-regulated during osteoblastic differentiation.2 Publications

Gene expression databases

BgeeiQ75UR0.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046884.

Structurei

3D structure databases

ProteinModelPortaliQ75UR0.
SMRiQ75UR0. Positions 250-748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi631 – 6366Poly-Arg

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ75UR0.
KOiK19480.
OMAiDFPNHTV.
OrthoDBiEOG7BS48W.
PhylomeDBiQ75UR0.
TreeFamiTF314265.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031294. Anoctamin-5.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PTHR12308:SF23. PTHR12308:SF23. 1 hit.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: At least 14 isoforms are produced using different combinations of exons 4, 6, 16, 20 and 21.

Isoform 1 (identifier: Q75UR0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEQEGLTAK EIDYAFQQNE NLGSKETSFL IPEDLQSPPE KRFNLFLRRR
60 70 80 90 100
LMFQRSEHSK DSVFFRDGIR QIDFVLSYVE DLKKDGELKA ERRREFEQNL
110 120 130 140 150
RKTGLDLETE DKLNSEDGKT YFVKIHAPWE VLVTYAEVLG IKMPIKLSDI
160 170 180 190 200
PRPKYPPLSY MLGAVKLPSS VKYPTPEYFT AQFSRHRQEL FLIEDEATFF
210 220 230 240 250
PSSTRNRIVY YILSRCPFGV EEGKKKIGIE RLLNSNTYLS AYPLHDGQYW
260 270 280 290 300
KPSKTTRPNE RYNLCKNWAR FSYFYKEQPF HLIRNYFGEK IGIYFVFLGY
310 320 330 340 350
YTEMLLFAAL VGLACFIYGL LSMENNRTST EICDPDIGGQ MIMCPLCDEV
360 370 380 390 400
CDYWRLNTTC LHSKFSHLFD NESTVFFALF MGIWVTLFLE FWKQRQARLE
410 420 430 440 450
YEWDLVDFEE EQQQLQLRPE FEAMCKHKKM NPVTKEMEPH MPLCHRIPWY
460 470 480 490 500
FVSGTTVTFG MALLLSSMVS ILIYRLSVFA TFASFMESEA TLQSVKSFFT
510 520 530 540 550
PQLATALSGS CLNCIVILIL NFFYEKISAW ITKMEIPRTH QEYESSLTLK
560 570 580 590 600
MFLFQFVNYY SSCFYVAFFK GKFVGYPGSY TYMFNIWRSE ECGPAGCLIE
610 620 630 640 650
LTTQLTIIMI GKQIFGNIHE AFQPLIFNWW RRRRARTHSE KLYSRWEQDH
660 670 680 690 700
DLQVYGHRGL FYEYLETVIQ FGFATLFVAS FPLAPLFALM NNIMGIRVDA
710 720 730 740 750
WKLTTQYRRP VAAKAHSIGV WQDILFGMAI VSVATNAFIV SFTSDIIPRL
760 770 780 790 800
VYFYAYSTNS TEPLSGYVNN SLSVFLIADF PNHTVPMEKK DFVTCRYRDY
810 820 830 840 850
RYPPDHEDKY SHNMQFWHVL AAKMTFIIVM EHVVFLFKFL LAWLIPDVPK
860 870 880 890 900
DVVEKIKREK LMTIKIIHDF ELNKLKENLD VEYGNIMKNV LVDEDNSLKA

KTTV
Length:904
Mass (Da):106,214
Last modified:July 5, 2004 - v1
Checksum:i347FA6BC33FA0042
GO
Isoform 2 (identifier: Q75UR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     34-52: DLQSPPEKRFNLFLRRRLM → MNLGSKETSFLIPEDLQSP
     796-805: RYRDYRYPPD → STSCFCLNFY
     806-904: Missing.

Show »
Length:772
Mass (Da):90,193
Checksum:iA45C0ED55281EDC1
GO
Isoform 3 (identifier: Q75UR0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-52: Missing.
     535-570: Missing.

Show »
Length:854
Mass (Da):99,908
Checksum:i8329249AEE571BA5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3333Missing in isoform 2. 1 PublicationVSP_015628Add
BLAST
Alternative sequencei34 – 5219DLQSP…RRRLM → MNLGSKETSFLIPEDLQSP in isoform 2. 1 PublicationVSP_015629Add
BLAST
Alternative sequencei39 – 5214Missing in isoform 3. 1 PublicationVSP_035571Add
BLAST
Alternative sequencei535 – 57036Missing in isoform 3. 1 PublicationVSP_035572Add
BLAST
Alternative sequencei796 – 80510RYRDYRYPPD → STSCFCLNFY in isoform 2. 1 PublicationVSP_015630
Alternative sequencei806 – 90499Missing in isoform 2. 1 PublicationVSP_015631Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125740 mRNA. Translation: BAD17873.1.
AB206762 mRNA. Translation: BAE44438.1.
AK034197 mRNA. Translation: BAC28628.1.
BC109163 mRNA. Translation: AAI09164.2.
CCDSiCCDS39968.1. [Q75UR0-1]
RefSeqiNP_001258808.1. NM_001271879.1. [Q75UR0-3]
NP_808362.2. NM_177694.6. [Q75UR0-1]
UniGeneiMm.254431.
Mm.411727.

Genome annotation databases

EnsembliENSMUST00000043944; ENSMUSP00000046884; ENSMUSG00000055489. [Q75UR0-1]
ENSMUST00000207044; ENSMUSP00000147243; ENSMUSG00000055489. [Q75UR0-2]
ENSMUST00000207717; ENSMUSP00000146783; ENSMUSG00000055489. [Q75UR0-3]
GeneIDi233246.
KEGGimmu:233246.
UCSCiuc009hcc.2. mouse. [Q75UR0-1]
uc009hce.2. mouse. [Q75UR0-2]
uc033izs.1. mouse. [Q75UR0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125740 mRNA. Translation: BAD17873.1.
AB206762 mRNA. Translation: BAE44438.1.
AK034197 mRNA. Translation: BAC28628.1.
BC109163 mRNA. Translation: AAI09164.2.
CCDSiCCDS39968.1. [Q75UR0-1]
RefSeqiNP_001258808.1. NM_001271879.1. [Q75UR0-3]
NP_808362.2. NM_177694.6. [Q75UR0-1]
UniGeneiMm.254431.
Mm.411727.

3D structure databases

ProteinModelPortaliQ75UR0.
SMRiQ75UR0. Positions 250-748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046884.

Protein family/group databases

TCDBi1.A.17.1.2. the calcium-dependent chloride channel (ca-clc) family.

PTM databases

PhosphoSiteiQ75UR0.

Proteomic databases

PaxDbiQ75UR0.
PRIDEiQ75UR0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043944; ENSMUSP00000046884; ENSMUSG00000055489. [Q75UR0-1]
ENSMUST00000207044; ENSMUSP00000147243; ENSMUSG00000055489. [Q75UR0-2]
ENSMUST00000207717; ENSMUSP00000146783; ENSMUSG00000055489. [Q75UR0-3]
GeneIDi233246.
KEGGimmu:233246.
UCSCiuc009hcc.2. mouse. [Q75UR0-1]
uc009hce.2. mouse. [Q75UR0-2]
uc033izs.1. mouse. [Q75UR0-3]

Organism-specific databases

CTDi203859.
MGIiMGI:3576659. Ano5.

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ75UR0.
KOiK19480.
OMAiDFPNHTV.
OrthoDBiEOG7BS48W.
PhylomeDBiQ75UR0.
TreeFamiTF314265.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

NextBioi381649.
PROiQ75UR0.
SOURCEiSearch...

Gene expression databases

BgeeiQ75UR0.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031294. Anoctamin-5.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PTHR12308:SF23. PTHR12308:SF23. 1 hit.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia (GDD)."
    Tsutsumi S., Kamata N., Vokes T.J., Maruoka Y., Nakakuki K., Enomoto S., Omura K., Amagasa T., Nagayama M., Saito-Ohara F., Inazawa J., Moritani M., Yamaoka T., Inoue H., Itakura M.
    Am. J. Hum. Genet. 74:1255-1261(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INDUCTION.
    Strain: BALB/cJ.
    Tissue: Skeletal muscle.
  2. "Molecular cloning and characterization of the murine gnathodiaphyseal dysplasia gene GDD1."
    Tsutsumi S., Inoue H., Sakamoto Y., Mizuta K., Kamata N., Itakura M.
    Biochem. Biophys. Res. Commun. 331:1099-1106(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INDUCTION.
    Strain: BDF1.
    Tissue: Skeletal muscle.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Diencephalon.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. "Molecular characterization of GDD1/TMEM16E, the gene product responsible for autosomal dominant gnathodiaphyseal dysplasia."
    Mizuta K., Tsutsumi S., Inoue H., Sakamoto Y., Miyatake K., Miyawaki K., Noji S., Kamata N., Itakura M.
    Biochem. Biophys. Res. Commun. 357:126-132(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. Cited for: TISSUE SPECIFICITY.
  7. "The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
    Winpenny J.P., Gray M.A.
    Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiANO5_MOUSE
AccessioniPrimary (citable) accession number: Q75UR0
Secondary accession number(s): Q2VPA8, Q3V657, Q8CC04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.