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Protein

Anoctamin-5

Gene

Ano5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Does not exhibit calcium-activated chloride channel (CaCC) activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.17.1.2. the calcium-dependent chloride channel (ca-clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-5
Alternative name(s):
Gnathodiaphyseal dysplasia 1 protein homolog
Transmembrane protein 16E
Gene namesi
Name:Ano5
Synonyms:Gdd1, Tmem16e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:3576659. Ano5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 290CytoplasmicSequence analysisAdd BLAST290
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 371ExtracellularSequence analysisAdd BLAST60
Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
Topological domaini393 – 453CytoplasmicSequence analysisAdd BLAST61
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 502ExtracellularSequence analysisAdd BLAST28
Transmembranei503 – 523HelicalSequence analysisAdd BLAST21
Topological domaini524 – 548CytoplasmicSequence analysisAdd BLAST25
Transmembranei549 – 569HelicalSequence analysisAdd BLAST21
Topological domaini570 – 667ExtracellularSequence analysisAdd BLAST98
Transmembranei668 – 688HelicalSequence analysisAdd BLAST21
Topological domaini689 – 723CytoplasmicSequence analysisAdd BLAST35
Transmembranei724 – 744HelicalSequence analysisAdd BLAST21
Topological domaini745 – 825ExtracellularSequence analysisAdd BLAST81
Transmembranei826 – 846HelicalSequence analysisAdd BLAST21
Topological domaini847 – 904CytoplasmicSequence analysisAdd BLAST58

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917561 – 904Anoctamin-5Add BLAST904

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi326N-linked (GlcNAc...)Sequence analysis1
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1
Glycosylationi759N-linked (GlcNAc...)Sequence analysis1
Glycosylationi769N-linked (GlcNAc...)Sequence analysis1
Glycosylationi782N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ75UR0.
PRIDEiQ75UR0.

PTM databases

iPTMnetiQ75UR0.
PhosphoSitePlusiQ75UR0.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, bone tissues and thyroid gland.4 Publications

Developmental stagei

In the developing embryo, expressed mainly in differentiating and developing somites and is associated with myotomal somite lineages.1 Publication

Inductioni

Up-regulated during the course of myogenic differentiation. Down-regulated during osteoblastic differentiation.2 Publications

Gene expression databases

BgeeiENSMUSG00000055489.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046884.

Structurei

3D structure databases

ProteinModelPortaliQ75UR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi631 – 636Poly-Arg6

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ75UR0.
KOiK19480.
OMAiFTTCRYR.
OrthoDBiEOG091G01JF.
PhylomeDBiQ75UR0.
TreeFamiTF314265.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031294. Anoctamin-5.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PTHR12308:SF23. PTHR12308:SF23. 1 hit.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: At least 14 isoforms are produced using different combinations of exons 4, 6, 16, 20 and 21.
Isoform 1 (identifier: Q75UR0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEQEGLTAK EIDYAFQQNE NLGSKETSFL IPEDLQSPPE KRFNLFLRRR
60 70 80 90 100
LMFQRSEHSK DSVFFRDGIR QIDFVLSYVE DLKKDGELKA ERRREFEQNL
110 120 130 140 150
RKTGLDLETE DKLNSEDGKT YFVKIHAPWE VLVTYAEVLG IKMPIKLSDI
160 170 180 190 200
PRPKYPPLSY MLGAVKLPSS VKYPTPEYFT AQFSRHRQEL FLIEDEATFF
210 220 230 240 250
PSSTRNRIVY YILSRCPFGV EEGKKKIGIE RLLNSNTYLS AYPLHDGQYW
260 270 280 290 300
KPSKTTRPNE RYNLCKNWAR FSYFYKEQPF HLIRNYFGEK IGIYFVFLGY
310 320 330 340 350
YTEMLLFAAL VGLACFIYGL LSMENNRTST EICDPDIGGQ MIMCPLCDEV
360 370 380 390 400
CDYWRLNTTC LHSKFSHLFD NESTVFFALF MGIWVTLFLE FWKQRQARLE
410 420 430 440 450
YEWDLVDFEE EQQQLQLRPE FEAMCKHKKM NPVTKEMEPH MPLCHRIPWY
460 470 480 490 500
FVSGTTVTFG MALLLSSMVS ILIYRLSVFA TFASFMESEA TLQSVKSFFT
510 520 530 540 550
PQLATALSGS CLNCIVILIL NFFYEKISAW ITKMEIPRTH QEYESSLTLK
560 570 580 590 600
MFLFQFVNYY SSCFYVAFFK GKFVGYPGSY TYMFNIWRSE ECGPAGCLIE
610 620 630 640 650
LTTQLTIIMI GKQIFGNIHE AFQPLIFNWW RRRRARTHSE KLYSRWEQDH
660 670 680 690 700
DLQVYGHRGL FYEYLETVIQ FGFATLFVAS FPLAPLFALM NNIMGIRVDA
710 720 730 740 750
WKLTTQYRRP VAAKAHSIGV WQDILFGMAI VSVATNAFIV SFTSDIIPRL
760 770 780 790 800
VYFYAYSTNS TEPLSGYVNN SLSVFLIADF PNHTVPMEKK DFVTCRYRDY
810 820 830 840 850
RYPPDHEDKY SHNMQFWHVL AAKMTFIIVM EHVVFLFKFL LAWLIPDVPK
860 870 880 890 900
DVVEKIKREK LMTIKIIHDF ELNKLKENLD VEYGNIMKNV LVDEDNSLKA

KTTV
Length:904
Mass (Da):106,214
Last modified:July 5, 2004 - v1
Checksum:i347FA6BC33FA0042
GO
Isoform 2 (identifier: Q75UR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     34-52: DLQSPPEKRFNLFLRRRLM → MNLGSKETSFLIPEDLQSP
     796-805: RYRDYRYPPD → STSCFCLNFY
     806-904: Missing.

Show »
Length:772
Mass (Da):90,193
Checksum:iA45C0ED55281EDC1
GO
Isoform 3 (identifier: Q75UR0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-52: Missing.
     535-570: Missing.

Show »
Length:854
Mass (Da):99,908
Checksum:i8329249AEE571BA5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0156281 – 33Missing in isoform 2. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_01562934 – 52DLQSP…RRRLM → MNLGSKETSFLIPEDLQSP in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_03557139 – 52Missing in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_035572535 – 570Missing in isoform 3. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_015630796 – 805RYRDYRYPPD → STSCFCLNFY in isoform 2. 1 Publication10
Alternative sequenceiVSP_015631806 – 904Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125740 mRNA. Translation: BAD17873.1.
AB206762 mRNA. Translation: BAE44438.1.
AK034197 mRNA. Translation: BAC28628.1.
BC109163 mRNA. Translation: AAI09164.2.
CCDSiCCDS39968.1. [Q75UR0-1]
RefSeqiNP_001258808.1. NM_001271879.1. [Q75UR0-3]
NP_808362.2. NM_177694.6. [Q75UR0-1]
UniGeneiMm.254431.
Mm.411727.

Genome annotation databases

EnsembliENSMUST00000043944; ENSMUSP00000046884; ENSMUSG00000055489. [Q75UR0-1]
ENSMUST00000207044; ENSMUSP00000147243; ENSMUSG00000055489. [Q75UR0-2]
ENSMUST00000207717; ENSMUSP00000146783; ENSMUSG00000055489. [Q75UR0-3]
GeneIDi233246.
KEGGimmu:233246.
UCSCiuc009hcc.2. mouse. [Q75UR0-1]
uc009hce.2. mouse. [Q75UR0-2]
uc033izs.1. mouse. [Q75UR0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125740 mRNA. Translation: BAD17873.1.
AB206762 mRNA. Translation: BAE44438.1.
AK034197 mRNA. Translation: BAC28628.1.
BC109163 mRNA. Translation: AAI09164.2.
CCDSiCCDS39968.1. [Q75UR0-1]
RefSeqiNP_001258808.1. NM_001271879.1. [Q75UR0-3]
NP_808362.2. NM_177694.6. [Q75UR0-1]
UniGeneiMm.254431.
Mm.411727.

3D structure databases

ProteinModelPortaliQ75UR0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046884.

Protein family/group databases

TCDBi1.A.17.1.2. the calcium-dependent chloride channel (ca-clc) family.

PTM databases

iPTMnetiQ75UR0.
PhosphoSitePlusiQ75UR0.

Proteomic databases

PaxDbiQ75UR0.
PRIDEiQ75UR0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043944; ENSMUSP00000046884; ENSMUSG00000055489. [Q75UR0-1]
ENSMUST00000207044; ENSMUSP00000147243; ENSMUSG00000055489. [Q75UR0-2]
ENSMUST00000207717; ENSMUSP00000146783; ENSMUSG00000055489. [Q75UR0-3]
GeneIDi233246.
KEGGimmu:233246.
UCSCiuc009hcc.2. mouse. [Q75UR0-1]
uc009hce.2. mouse. [Q75UR0-2]
uc033izs.1. mouse. [Q75UR0-3]

Organism-specific databases

CTDi203859.
MGIiMGI:3576659. Ano5.

Phylogenomic databases

eggNOGiKOG2514. Eukaryota.
ENOG410XS4S. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ75UR0.
KOiK19480.
OMAiFTTCRYR.
OrthoDBiEOG091G01JF.
PhylomeDBiQ75UR0.
TreeFamiTF314265.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ75UR0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055489.

Family and domain databases

InterProiIPR032394. Anoct_dimer.
IPR007632. Anoctamin.
IPR031294. Anoctamin-5.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PTHR12308:SF23. PTHR12308:SF23. 1 hit.
PfamiPF16178. Anoct_dimer. 1 hit.
PF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANO5_MOUSE
AccessioniPrimary (citable) accession number: Q75UR0
Secondary accession number(s): Q2VPA8, Q3V657, Q8CC04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.