Reviewed, UniProtKB/Swiss-Prot Q75UG4 (P4HA3_BOVIN)

Names and origin

Protein names

Recommended name:
    Prolyl 4-hydroxylase subunit alpha-3
    EC=1.14.11.2
Alternative name(s):
    4-PH alpha-3
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3

Gene names

Name:

P4HA3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.
Catalytic activity	Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-4-hydroxy-L-proline + succinate + CO(2).
Cofactor	Binds 1 Fe(2+) ion per subunit By similarity. Ascorbate By similarity.
Subunit structure	Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.
Subcellular location	Endoplasmic reticulum lumenBy similarity.
Post-translational modification	N-glycosylation plays no role in the catalytic activity By similarity.
Sequence similarities	Belongs to the P4HA family. Contains 1 PKHD (prolyl/lysyl hydroxylase) domain. Contains 1 TPR repeat.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Coiled coil Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 4-dioxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 544	525	Prolyl 4-hydroxylase subunit alpha-3		PRO_0000317765
Regions
	Repeat	227 – 260	34	TPR
	Domain	422 – 529	108	PKHD
	Coiled coil	107 – 131	25	Potential
Sites
	Metal binding	440	1	Iron By similarity
	Metal binding	442	1	Iron By similarity
	Metal binding	510	1	Iron By similarity
	Binding site	520	1	2-oxoglutarate Potential
Amino acid modifications
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Glycosylation	482	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75UG4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D996D34D44A6F230	FASTA	544	61,023
	10 20 30 40 50 60 MGPAARLAAL LAVLAFRAGD PAEVAARGDT FSALTSVARA LAPERRLLGL LRRYLRGEEA 70 80 90 100 110 120 RLRDLTRFYH KVLSLHEDSA TPVSNPLLAF TLIKRLQSDW KNVVHSLEAS ENIRALKDGY 130 140 150 160 170 180 ERVEQDLPAF EDLEGAARAL MRLQDVYMLN VKGLARGVFQ RVTGSAVTDL YSPRRLFSLT 190 200 210 220 230 240 GDDCFQVGKV AYDMGDYYHA IPWLEEAVSL FRGSYGEWKT EDEASLEDAL DHLAFAYFQA 250 260 270 280 290 300 GNVLCALNLS REFLLYSPDN KRVARNVLKY EKLLAESPNQ AVAETVMQRP NVPHLQTRDT 310 320 330 340 350 360 YEGLCQTLGS QPTHYRIPSL YCSYETSSSP YLLLQPVRKE VIHLEPYVVL YHDFVSDAEA 370 380 390 400 410 420 QTIRGLAEPW LQRSVVASGE KQLPVEYRIS KSAWLKDTVD PVLVTLDHRI AALTGLDVQP 430 440 450 460 470 480 PYAEYLQVVN YGIGGHYEPH FDHATSPSSP LYRMNSGNRV ATFMIYLSSV EAGGATAFIY 490 500 510 520 530 540 GNFSVPVVKN AALFWWNLHR SGEGDGDTLH AACPVLVGDK WVANKWIHEY GQEFRRPCSS RPED

References

[1]	"Cloning and expression of collagen prolyl 4-hydroxylase during bovine adipogenesis." Tahara K., Aso H., Yamasaki T., Takano S. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB126035 mRNA. Translation: BAD18888.1.
RefSeq	NP_001001598.1.
UniGene	Bt.88095
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000006579. Bos taurus. [Contig view]
GeneID	414348.
KEGG	bta:414348.
Phylogenomic databases
HOVERGEN	Q75UG4.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR006620. Pro_4_hyd_alph. IPR013547. Pro_4_hyd_alph_N. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF08336. P4Ha_N. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P4HA3_BOVIN

Accession

Primary (citable) accession number: Q75UG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)