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Protein

Prolyl 4-hydroxylase subunit alpha-3

Gene

P4HA3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi440IronPROSITE-ProRule annotation1
Metal bindingi442IronPROSITE-ProRule annotation1
Metal bindingi510IronPROSITE-ProRule annotation1
Binding sitei5202-oxoglutaratePROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-BTA-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 4-hydroxylase subunit alpha-3 (EC:1.14.11.2)
Short name:
4-PH alpha-3
Alternative name(s):
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3
Gene namesi
Name:P4HA3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

Organism-specific databases

VGNCiVGNC:32537 P4HA3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000031776520 – 544Prolyl 4-hydroxylase subunit alpha-3Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi482N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylation plays no role in the catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ75UG4
PRIDEiQ75UG4

Expressioni

Gene expression databases

BgeeiENSBTAG00000006579

Interactioni

Subunit structurei

Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008644

Structurei

3D structure databases

ProteinModelPortaliQ75UG4
SMRiQ75UG4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati227 – 260TPRAdd BLAST34
Domaini422 – 529Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST108

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili107 – 131Sequence analysisAdd BLAST25

Sequence similaritiesi

Belongs to the P4HA family.Curated

Keywords - Domaini

Coiled coil, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG1591 Eukaryota
ENOG410XS5J LUCA
GeneTreeiENSGT00390000018885
HOGENOMiHOG000230465
HOVERGENiHBG006834
InParanoidiQ75UG4
KOiK00472
OMAiLQSDWRN
OrthoDBiEOG091G0749
TreeFamiTF313393

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR013547 Pro_4_hyd_alph_N
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF13640 2OG-FeII_Oxy_3, 1 hit
PF08336 P4Ha_N, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75UG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPAARLAAL LAVLAFRAGD PAEVAARGDT FSALTSVARA LAPERRLLGL
60 70 80 90 100
LRRYLRGEEA RLRDLTRFYH KVLSLHEDSA TPVSNPLLAF TLIKRLQSDW
110 120 130 140 150
KNVVHSLEAS ENIRALKDGY ERVEQDLPAF EDLEGAARAL MRLQDVYMLN
160 170 180 190 200
VKGLARGVFQ RVTGSAVTDL YSPRRLFSLT GDDCFQVGKV AYDMGDYYHA
210 220 230 240 250
IPWLEEAVSL FRGSYGEWKT EDEASLEDAL DHLAFAYFQA GNVLCALNLS
260 270 280 290 300
REFLLYSPDN KRVARNVLKY EKLLAESPNQ AVAETVMQRP NVPHLQTRDT
310 320 330 340 350
YEGLCQTLGS QPTHYRIPSL YCSYETSSSP YLLLQPVRKE VIHLEPYVVL
360 370 380 390 400
YHDFVSDAEA QTIRGLAEPW LQRSVVASGE KQLPVEYRIS KSAWLKDTVD
410 420 430 440 450
PVLVTLDHRI AALTGLDVQP PYAEYLQVVN YGIGGHYEPH FDHATSPSSP
460 470 480 490 500
LYRMNSGNRV ATFMIYLSSV EAGGATAFIY GNFSVPVVKN AALFWWNLHR
510 520 530 540
SGEGDGDTLH AACPVLVGDK WVANKWIHEY GQEFRRPCSS RPED
Length:544
Mass (Da):61,023
Last modified:July 5, 2004 - v1
Checksum:iD996D34D44A6F230
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB126035 mRNA Translation: BAD18888.1
RefSeqiNP_001001598.1, NM_001001598.2
UniGeneiBt.88095

Genome annotation databases

EnsembliENSBTAT00000008644; ENSBTAP00000008644; ENSBTAG00000006579
GeneIDi414348
KEGGibta:414348

Similar proteinsi

Entry informationi

Entry nameiP4HA3_BOVIN
AccessioniPrimary (citable) accession number: Q75UG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health