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Q75T13

- PGAP1_HUMAN

UniProt

Q75T13 - PGAP1_HUMAN

Protein

GPI inositol-deacylase

Gene

PGAP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741By similarity

    GO - Molecular functioni

    1. nuclease activity Source: UniProtKB
    2. phosphoric ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior axis specification Source: Ensembl
    2. attachment of GPI anchor to protein Source: Reactome
    3. cellular protein metabolic process Source: Reactome
    4. C-terminal protein lipidation Source: Reactome
    5. embryonic pattern specification Source: Ensembl
    6. forebrain regionalization Source: Ensembl
    7. head development Source: Ensembl
    8. intracellular protein transport Source: InterPro
    9. myo-inositol transport Source: UniProtKB
    10. post-translational protein modification Source: Reactome
    11. sensory perception of sound Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_1830. Attachment of GPI anchor to uPAR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI inositol-deacylase (EC:3.1.-.-)
    Alternative name(s):
    Post-GPI attachment to proteins factor 1
    Short name:
    hPGAP1
    Gene namesi
    Name:PGAP1
    ORF Names:UNQ3024/PRO9822
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:25712. PGAP1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162399235.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 922922GPI inositol-deacylasePRO_0000277623Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi558 – 5581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ75T13.
    PaxDbiQ75T13.
    PRIDEiQ75T13.

    PTM databases

    PhosphoSiteiQ75T13.

    Expressioni

    Gene expression databases

    ArrayExpressiQ75T13.
    BgeeiQ75T13.
    CleanExiHS_PGAP1.
    GenevestigatoriQ75T13.

    Interactioni

    Protein-protein interaction databases

    BioGridi123093. 4 interactions.
    IntActiQ75T13. 2 interactions.
    MINTiMINT-4828389.
    STRINGi9606.ENSP00000346809.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75T13.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 641609LumenalSequence AnalysisAdd
    BLAST
    Topological domaini663 – 6686CytoplasmicSequence Analysis
    Topological domaini690 – 73344LumenalSequence AnalysisAdd
    BLAST
    Topological domaini755 – 81763CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini839 – 85315LumenalSequence AnalysisAdd
    BLAST
    Topological domaini875 – 89319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini915 – 9228LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei642 – 66221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei669 – 68921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei734 – 75421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei818 – 83821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei854 – 87421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei894 – 91421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI inositol-deacylase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG312611.
    HOVERGENiHBG082122.
    InParanoidiQ75T13.
    KOiK05294.
    OMAiTKFVHEC.
    OrthoDBiEOG7KM5RZ.
    PhylomeDBiQ75T13.
    TreeFamiTF314565.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR012908. PGAP1-like.
    [Graphical view]
    PfamiPF07819. PGAP1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q75T13-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK    50
    IELPKKLAKR YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ 100
    VRSIGSIALR KAEDIDFKYH FDFFSVNFNE ELVALYGGSL QKQTKFVHEC 150
    IKTILKLYKG QEFAPKSVAI IGHSMGGLVA RALLTLKNFK HDLINLLITQ 200
    ATPHVAPVMP LDRFITDFYT TVNNYWILNA RHINLTTLSV AGGFRDYQVR 250
    SGLTFLPKLS HHTSALSVVS SAVPKTWVST DHLSIVWCKQ LQLTTVRAFF 300
    DLIDADTKQI TQNSKKKLSV LYHHFIRHPS KHFEENPAII SDLTGTSMWV 350
    LVKVSKWTYV AYNESEKIYF TFPLENHRKI YTHVYCQSTM LDTNSWIFAC 400
    INSTSMCLQG VDLSWKAELL PTIKYLTLRL QDYPSLSHLV VYVPSVRGSK 450
    FVVDCEFFKK EKRYIQLPVT HLFSFGLSSR KVVLNTNGLY YNLELLNFGQ 500
    IYQAFKINVV SKCSAVKEEI TSIYRLHIPW SYEDSLTIAQ APSSTEISLK 550
    LHIAQPENNT HVALFKMYTS SDCRYEVTVK TSFSQILGQV VRFHGGALPA 600
    YVVSNILLAY RGQLYSLFST GCCLEYATML DKEAKPYKVD PFVIIIKFLL 650
    GYKWFKELWD VLLLPELDAV ILTCQSMCFP LISLILFLFG TCTAYWSGLL 700
    SSASVRLLSS LWLALKRPSE LPKDIKMISP DLPFLTIVLI IVSWTTCGAL 750
    AILLSYLYYV FKVVHLQASL TTFKNSQPVN PKHSRRSEKK SNHHKDSSIH 800
    HLRLSANDAE DSLRMHSTVI NLLTWIVLLS MPSLIYWLKN LRYYFKLNPD 850
    PCKPLAFILI PTMAILGNTY TVSIKSSKLL KTTSQFPLPL AVGVIAFGSA 900
    HLYRLPCFVF IPLLLHALCN FM 922
    Length:922
    Mass (Da):105,383
    Last modified:July 5, 2004 - v1
    Checksum:i0305D18F5BF292D6
    GO
    Isoform 2 (identifier: Q75T13-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-174: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:748
    Mass (Da):85,239
    Checksum:i5C67D37891F3FAAC
    GO
    Isoform 3 (identifier: Q75T13-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         592-592: R → A
         593-922: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:592
    Mass (Da):67,974
    Checksum:iBD6B7BB40B8A0BC1
    GO
    Isoform 4 (identifier: Q75T13-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         50-269: Missing.
         652-922: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:431
    Mass (Da):49,845
    Checksum:i86F82D676245A7E3
    GO

    Sequence cautioni

    The sequence BC040517 differs from that shown. Reason: Frameshift at position 333.
    The sequence AAQ88987.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti304 – 3041D → G in BAB14035. (PubMed:14702039)Curated
    Sequence conflicti389 – 3891T → A in CAH10543. (PubMed:17974005)Curated
    Sequence conflicti701 – 7011S → P in CAH10543. (PubMed:17974005)Curated
    Sequence conflicti809 – 8091A → T in CAH10543. (PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 174174Missing in isoform 2. 1 PublicationVSP_023040Add
    BLAST
    Alternative sequencei50 – 269220Missing in isoform 4. 1 PublicationVSP_023041Add
    BLAST
    Alternative sequencei592 – 5921R → A in isoform 3. 1 PublicationVSP_023042
    Alternative sequencei593 – 922330Missing in isoform 3. 1 PublicationVSP_023043Add
    BLAST
    Alternative sequencei652 – 922271Missing in isoform 4. 1 PublicationVSP_023044Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB128038 mRNA. Translation: BAD13427.1.
    AK022439 mRNA. Translation: BAB14035.1.
    BX648642 mRNA. Translation: CAH10543.1.
    AC017035 Genomic DNA. Translation: AAY15059.1.
    AC012486 Genomic DNA. Translation: AAX88854.1.
    BC040517 mRNA. No translation available.
    AY358624 mRNA. Translation: AAQ88987.1. Different initiation.
    CCDSiCCDS2318.1. [Q75T13-1]
    RefSeqiNP_079265.2. NM_024989.3. [Q75T13-1]
    XP_005246923.1. XM_005246866.1. [Q75T13-2]
    UniGeneiHs.229988.

    Genome annotation databases

    EnsembliENST00000354764; ENSP00000346809; ENSG00000197121. [Q75T13-1]
    ENST00000409475; ENSP00000387028; ENSG00000197121. [Q75T13-3]
    GeneIDi80055.
    KEGGihsa:80055.
    UCSCiuc002utw.3. human. [Q75T13-1]
    uc002uty.1. human. [Q75T13-3]

    Polymorphism databases

    DMDMi74758940.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB128038 mRNA. Translation: BAD13427.1 .
    AK022439 mRNA. Translation: BAB14035.1 .
    BX648642 mRNA. Translation: CAH10543.1 .
    AC017035 Genomic DNA. Translation: AAY15059.1 .
    AC012486 Genomic DNA. Translation: AAX88854.1 .
    BC040517 mRNA. No translation available.
    AY358624 mRNA. Translation: AAQ88987.1 . Different initiation.
    CCDSi CCDS2318.1. [Q75T13-1 ]
    RefSeqi NP_079265.2. NM_024989.3. [Q75T13-1 ]
    XP_005246923.1. XM_005246866.1. [Q75T13-2 ]
    UniGenei Hs.229988.

    3D structure databases

    ProteinModelPortali Q75T13.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123093. 4 interactions.
    IntActi Q75T13. 2 interactions.
    MINTi MINT-4828389.
    STRINGi 9606.ENSP00000346809.

    PTM databases

    PhosphoSitei Q75T13.

    Polymorphism databases

    DMDMi 74758940.

    Proteomic databases

    MaxQBi Q75T13.
    PaxDbi Q75T13.
    PRIDEi Q75T13.

    Protocols and materials databases

    DNASUi 80055.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000354764 ; ENSP00000346809 ; ENSG00000197121 . [Q75T13-1 ]
    ENST00000409475 ; ENSP00000387028 ; ENSG00000197121 . [Q75T13-3 ]
    GeneIDi 80055.
    KEGGi hsa:80055.
    UCSCi uc002utw.3. human. [Q75T13-1 ]
    uc002uty.1. human. [Q75T13-3 ]

    Organism-specific databases

    CTDi 80055.
    GeneCardsi GC02M197697.
    HGNCi HGNC:25712. PGAP1.
    MIMi 611655. gene.
    neXtProti NX_Q75T13.
    PharmGKBi PA162399235.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG312611.
    HOVERGENi HBG082122.
    InParanoidi Q75T13.
    KOi K05294.
    OMAi TKFVHEC.
    OrthoDBi EOG7KM5RZ.
    PhylomeDBi Q75T13.
    TreeFami TF314565.

    Enzyme and pathway databases

    Reactomei REACT_1830. Attachment of GPI anchor to uPAR.

    Miscellaneous databases

    GenomeRNAii 80055.
    NextBioi 70254.
    PROi Q75T13.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q75T13.
    Bgeei Q75T13.
    CleanExi HS_PGAP1.
    Genevestigatori Q75T13.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR012908. PGAP1-like.
    [Graphical view ]
    Pfami PF07819. PGAP1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p."
      Tanaka S., Maeda Y., Tashima Y., Kinoshita T.
      J. Biol. Chem. 279:14256-14263(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Mammary gland.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Fetal kidney.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
      Tissue: Testis.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 705-922 (ISOFORMS 1/2).

    Entry informationi

    Entry nameiPGAP1_HUMAN
    AccessioniPrimary (citable) accession number: Q75T13
    Secondary accession number(s): Q4G0R8
    , Q4ZG47, Q53SM0, Q6AW92, Q6UWV4, Q9HA24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3