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Q75R65

- JAK2_CHICK

UniProt

Q75R65 - JAK2_CHICK

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Protein

Tyrosine-protein kinase JAK2

Gene

JAK2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. In the cytoplasm, plays a pivotal role in signal transduction via its association with cytokine receptors, which constitutes an initiating step in signaling for many members of the cytokine receptor superfamily. Following stimulation with erythropoietin (EPO) during erythropoiesis, it is autophosphorylated and activated, leading to its association with erythropoietin receptor (EPOR) and tyrosine phosphorylation of residues in the EPOR cytoplasmic domain. Also involved in promoting the localization of EPOR to the plasma membrane. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Regulated by autophosphorylation, can both activate or decrease activity. Heme regulates its activity by enhancing the phosphorylation on Tyr-1004 and Tyr-1005 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei879 – 8791ATPPROSITE-ProRule annotation
Active sitei973 – 9731Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi852 – 8609ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. heme binding Source: UniProtKB
  3. histone binding Source: UniProtKB
  4. histone kinase activity (H3-Y41 specific) Source: UniProtKB
  5. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  6. protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. activation of JAK2 kinase activity Source: UniProtKB
  2. cytokine-mediated signaling pathway Source: UniProtKB
  3. erythrocyte differentiation Source: UniProtKB
  4. histone H3-Y41 phosphorylation Source: UniProtKB
  5. JAK-STAT cascade involved in growth hormone signaling pathway Source: UniProtKB
  6. protein autophosphorylation Source: UniProtKB
  7. signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK2 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 2
Short name:
JAK-2
Gene namesi
Name:JAK2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Endomembrane system By similarity; Peripheral membrane protein By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoskeleton Source: InterPro
  2. membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11291129Tyrosine-protein kinase JAK2PRO_0000324095Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei117 – 1171Phosphotyrosine; by autocatalysisBy similarity
Modified residuei865 – 8651Phosphotyrosine; by autocatalysisBy similarity
Modified residuei963 – 9631Phosphotyrosine; by autocatalysisBy similarity
Modified residuei969 – 9691Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1004 – 10041Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1005 – 10051Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated, leading to regulate its activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ75R65.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000037587.

Structurei

3D structure databases

ProteinModelPortaliQ75R65.
SMRiQ75R65. Positions 837-1129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 378344FERMPROSITE-ProRule annotationAdd
BLAST
Domaini399 – 48082SH2; atypicalPROSITE-ProRule annotationAdd
BLAST
Domaini542 – 806265Protein kinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini846 – 1118273Protein kinase 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 2 protein kinase domains.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiQ75R65.
KOiK04447.
PhylomeDBiQ75R65.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020693. Tyr_kinase_non-rcpt_Jak2.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
PF00017. SH2. 1 hit.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01825. JANUSKINASE2.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75R65-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACLTMANIE GSATATVHQN GDILGNTSAP KQTEPLLQVY LYYSPGKTGG
60 70 80 90 100
DYLQFPAGEY VAEEICIVAC KACGIMPVYH NMFALMSETE RVWYPPNHIF
110 120 130 140 150
HVDEATRLKL LYRIRFYFPH WYCNGTSRAC RYGIIRGSES PVLDDLVMSY
160 170 180 190 200
LFAQWRKDFL DGWIQMPVTH ETQEECLGMA VLDMMRVAKE KDQTPLAVYN
210 220 230 240 250
SVSYKMFLPK CVRAKIQEYH ILTRKRIRYR FRKFIQQFGQ CKATARNLKL
260 270 280 290 300
KYLINLETLQ SAFYSEVFEV KEPGGDPSGE ESFATIVITG NGGIQCSRGK
310 320 330 340 350
LKNCETLAEQ DLQTYCDFPD IIDVSIKQAS QEGSSERRIV TIHKQDSKNL
360 370 380 390 400
EAEFQSLREA LSFVSLIDGY YRLTADAHHY LCKEVAPPSV LENIQSNCHG
410 420 430 440 450
PIFMDFAINK LKKAGNQTGF YVLRCSPKDF KKYFLTFAIE RENATDYKHC
460 470 480 490 500
LITKNENGEY NLSGTKRSFG NLKDLLTCYQ TETVRSDSII FQFIKCCPPK
510 520 530 540 550
PKDKSNLLVF RSNSVSDVPS SPTLQRHNVS QMVFHKIRNE DLIFEESLGQ
560 570 580 590 600
GTFTKIFKGI RKEVGDYGQL HQTEVLLKVL DKVHRNYSES FFEAASMMSQ
610 620 630 640 650
LSYKHLVLNY GVCVCGEENI LVQEYVKFGS LDTYLKKNKN VINILWKLEV
660 670 680 690 700
AKQLALAMHF LEDKGLVHGN VCAKNILLIR EEDRKSGNLP FIKLSDPGIS
710 720 730 740 750
ITVLPRDILL ERIPWVPPEC IENPRQLSLA TDKWSFGTTL WEICSGGDKP
760 770 780 790 800
LSALDSSRKL QFYEDRHQLP APNWTELANL INNCMDYEPD FRPSFRAIIR
810 820 830 840 850
DLNSLFTPDY ELLTENDMLP NIRTGALGFS GAFEDRDPTQ FEERHLKFLQ
860 870 880 890 900
QLGKGNFGSV EMCRYDPLQD NTGEVVAVKK LQHSTEEHLR DFEREIEILK
910 920 930 940 950
SLQHDNIVKY KGVCYSAGRR NLRLIMEYLP YGSLRDYLQK HKERLDHKKL
960 970 980 990 1000
LLYASQICKG MEYLGTKRYV HRDLATRNIL VENENRVKIG DFGLTKVLPQ
1010 1020 1030 1040 1050
DKEYYKVKEP GESPIFWYAP ESLTESKFSV ASDVWSFGVV LYELFTYIEK
1060 1070 1080 1090 1100
SKSPPAEFMR MIGNDKQGQM IVFHLIELLK NNGRLPRPDG CPDEIYAIMK
1110 1120
ECWNNNVAQR PTFRDLAQRV DQIRDNMGG
Length:1,129
Mass (Da):129,847
Last modified:July 5, 2004 - v1
Checksum:i1631EE82D331B9BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB159042 mRNA. Translation: BAD07298.1.
RefSeqiNP_001025709.1. NM_001030538.1.
UniGeneiGga.954.

Genome annotation databases

GeneIDi374199.
KEGGigga:374199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB159042 mRNA. Translation: BAD07298.1 .
RefSeqi NP_001025709.1. NM_001030538.1.
UniGenei Gga.954.

3D structure databases

ProteinModelPortali Q75R65.
SMRi Q75R65. Positions 837-1129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000037587.

Proteomic databases

PaxDbi Q75R65.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374199.
KEGGi gga:374199.

Organism-specific databases

CTDi 3717.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000049158.
HOVERGENi HBG006195.
InParanoidi Q75R65.
KOi K04447.
PhylomeDBi Q75R65.

Miscellaneous databases

NextBioi 20813703.
PROi Q75R65.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020693. Tyr_kinase_non-rcpt_Jak2.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 2 hits.
PF00017. SH2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000636. TyrPK_Jak. 1 hit.
PRINTSi PR01823. JANUSKINASE.
PR01825. JANUSKINASE2.
PR00109. TYRKINASE.
SMARTi SM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view ]
SUPFAMi SSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEi PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "molecular cloning and characterization of chicken tyrosine kinase JAK-2."
    Sayed A.A., Horiuchi H., Furusawa S., Matsuda H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: White leghorn.
    Tissue: Spleen.

Entry informationi

Entry nameiJAK2_CHICK
AccessioniPrimary (citable) accession number: Q75R65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3