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Q75R65 (JAK2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase JAK2

EC=2.7.10.2
Alternative name(s):
Janus kinase 2
Short name=JAK-2
Gene names
Name:JAK2
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length1129 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. In the cytoplasm, plays a pivotal role in signal transduction via its association with cytokine receptors, which constitutes an initiating step in signaling for many members of the cytokine receptor superfamily. Following stimulation with erythropoietin (EPO) during erythropoiesis, it is autophosphorylated and activated, leading to its association with erythropoietin receptor (EPOR) and tyrosine phosphorylation of residues in the EPOR cytoplasmic domain. Also involved in promoting the localization of EPOR to the plasma membrane. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation

Regulated by autophosphorylation, can both activate or decrease activity. Heme regulates its activity by enhancing the phosphorylation on Tyr-1004 and Tyr-1005 By similarity.

Subcellular location

Endomembrane system; Peripheral membrane protein By similarity. Nucleus By similarity.

Domain

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1 By similarity.

Post-translational modification

Autophosphorylated, leading to regulate its activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Contains 1 FERM domain.

Contains 2 protein kinase domains.

Contains 1 SH2 domain.

Ontologies

Keywords
   Cellular componentMembrane
Nucleus
   DomainRepeat
SH2 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionChromatin regulator
Kinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJAK-STAT cascade involved in growth hormone signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

activation of JAK2 kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

cytokine-mediated signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

erythrocyte differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

histone H3-Y41 phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

protein autophosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

signal transduction

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: InterPro

endomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from sequence or structural similarity. Source: UniProtKB

histone binding

Inferred from sequence or structural similarity. Source: UniProtKB

histone kinase activity (H3-Y41 specific)

Inferred from sequence or structural similarity. Source: UniProtKB

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein tyrosine kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11291129Tyrosine-protein kinase JAK2
PRO_0000324095

Regions

Domain35 – 378344FERM
Domain399 – 48082SH2; atypical
Domain542 – 806265Protein kinase 1
Domain846 – 1118273Protein kinase 2
Nucleotide binding852 – 8609ATP By similarity

Sites

Active site9731Proton acceptor By similarity
Binding site8791ATP By similarity

Amino acid modifications

Modified residue1171Phosphotyrosine; by autocatalysis By similarity
Modified residue8651Phosphotyrosine; by autocatalysis By similarity
Modified residue9631Phosphotyrosine; by autocatalysis By similarity
Modified residue9691Phosphotyrosine; by autocatalysis By similarity
Modified residue10041Phosphotyrosine; by autocatalysis By similarity
Modified residue10051Phosphotyrosine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q75R65 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1631EE82D331B9BE

FASTA1,129129,847
        10         20         30         40         50         60 
MACLTMANIE GSATATVHQN GDILGNTSAP KQTEPLLQVY LYYSPGKTGG DYLQFPAGEY 

        70         80         90        100        110        120 
VAEEICIVAC KACGIMPVYH NMFALMSETE RVWYPPNHIF HVDEATRLKL LYRIRFYFPH 

       130        140        150        160        170        180 
WYCNGTSRAC RYGIIRGSES PVLDDLVMSY LFAQWRKDFL DGWIQMPVTH ETQEECLGMA 

       190        200        210        220        230        240 
VLDMMRVAKE KDQTPLAVYN SVSYKMFLPK CVRAKIQEYH ILTRKRIRYR FRKFIQQFGQ 

       250        260        270        280        290        300 
CKATARNLKL KYLINLETLQ SAFYSEVFEV KEPGGDPSGE ESFATIVITG NGGIQCSRGK 

       310        320        330        340        350        360 
LKNCETLAEQ DLQTYCDFPD IIDVSIKQAS QEGSSERRIV TIHKQDSKNL EAEFQSLREA 

       370        380        390        400        410        420 
LSFVSLIDGY YRLTADAHHY LCKEVAPPSV LENIQSNCHG PIFMDFAINK LKKAGNQTGF 

       430        440        450        460        470        480 
YVLRCSPKDF KKYFLTFAIE RENATDYKHC LITKNENGEY NLSGTKRSFG NLKDLLTCYQ 

       490        500        510        520        530        540 
TETVRSDSII FQFIKCCPPK PKDKSNLLVF RSNSVSDVPS SPTLQRHNVS QMVFHKIRNE 

       550        560        570        580        590        600 
DLIFEESLGQ GTFTKIFKGI RKEVGDYGQL HQTEVLLKVL DKVHRNYSES FFEAASMMSQ 

       610        620        630        640        650        660 
LSYKHLVLNY GVCVCGEENI LVQEYVKFGS LDTYLKKNKN VINILWKLEV AKQLALAMHF 

       670        680        690        700        710        720 
LEDKGLVHGN VCAKNILLIR EEDRKSGNLP FIKLSDPGIS ITVLPRDILL ERIPWVPPEC 

       730        740        750        760        770        780 
IENPRQLSLA TDKWSFGTTL WEICSGGDKP LSALDSSRKL QFYEDRHQLP APNWTELANL 

       790        800        810        820        830        840 
INNCMDYEPD FRPSFRAIIR DLNSLFTPDY ELLTENDMLP NIRTGALGFS GAFEDRDPTQ 

       850        860        870        880        890        900 
FEERHLKFLQ QLGKGNFGSV EMCRYDPLQD NTGEVVAVKK LQHSTEEHLR DFEREIEILK 

       910        920        930        940        950        960 
SLQHDNIVKY KGVCYSAGRR NLRLIMEYLP YGSLRDYLQK HKERLDHKKL LLYASQICKG 

       970        980        990       1000       1010       1020 
MEYLGTKRYV HRDLATRNIL VENENRVKIG DFGLTKVLPQ DKEYYKVKEP GESPIFWYAP 

      1030       1040       1050       1060       1070       1080 
ESLTESKFSV ASDVWSFGVV LYELFTYIEK SKSPPAEFMR MIGNDKQGQM IVFHLIELLK 

      1090       1100       1110       1120 
NNGRLPRPDG CPDEIYAIMK ECWNNNVAQR PTFRDLAQRV DQIRDNMGG 

« Hide

References

[1]"molecular cloning and characterization of chicken tyrosine kinase JAK-2."
Sayed A.A., Horiuchi H., Furusawa S., Matsuda H.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: White leghorn.
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB159042 mRNA. Translation: BAD07298.1.
RefSeqNP_001025709.1. NM_001030538.1.
UniGeneGga.954.

3D structure databases

ProteinModelPortalQ75R65.
SMRQ75R65. Positions 837-1129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000037587.

Proteomic databases

PaxDbQ75R65.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID374199.
KEGGgga:374199.

Organism-specific databases

CTD3717.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000049158.
HOVERGENHBG006195.
KOK04447.
PhylomeDBQ75R65.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020693. Tyr_kinase_non-rcpt_Jak2.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 2 hits.
PF00017. SH2. 1 hit.
[Graphical view]
PIRSFPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSPR01823. JANUSKINASE.
PR01825. JANUSKINASE2.
PR00109. TYRKINASE.
SMARTSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20813703.
PROQ75R65.

Entry information

Entry nameJAK2_CHICK
AccessionPrimary (citable) accession number: Q75R65
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families