Reviewed, UniProtKB/Swiss-Prot Q75R65 (JAK2_CHICK)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase JAK2
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 2
      Short name=JAK-2

Gene names

Name:

JAK2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1129 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.
Domain	Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	tyrosine phosphorylation of STAT protein Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1129	1129	Tyrosine-protein kinase JAK2		PRO_0000324095
Regions
	Domain	35 – 378	344	FERM
	Domain	399 – 480	82	SH2; atypical
	Domain	542 – 806	265	Protein kinase 1
	Domain	846 – 1118	273	Protein kinase 2
	Nucleotide binding	852 – 860	9	ATP By similarity
Sites
	Active site	973	1	Proton acceptor By similarity
	Binding site	879	1	ATP By similarity
Amino acid modifications
	Modified residue	1004	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75R65-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1631EE82D331B9BE	FASTA	1,129	129,847
	10 20 30 40 50 60 MACLTMANIE GSATATVHQN GDILGNTSAP KQTEPLLQVY LYYSPGKTGG DYLQFPAGEY 70 80 90 100 110 120 VAEEICIVAC KACGIMPVYH NMFALMSETE RVWYPPNHIF HVDEATRLKL LYRIRFYFPH 130 140 150 160 170 180 WYCNGTSRAC RYGIIRGSES PVLDDLVMSY LFAQWRKDFL DGWIQMPVTH ETQEECLGMA 190 200 210 220 230 240 VLDMMRVAKE KDQTPLAVYN SVSYKMFLPK CVRAKIQEYH ILTRKRIRYR FRKFIQQFGQ 250 260 270 280 290 300 CKATARNLKL KYLINLETLQ SAFYSEVFEV KEPGGDPSGE ESFATIVITG NGGIQCSRGK 310 320 330 340 350 360 LKNCETLAEQ DLQTYCDFPD IIDVSIKQAS QEGSSERRIV TIHKQDSKNL EAEFQSLREA 370 380 390 400 410 420 LSFVSLIDGY YRLTADAHHY LCKEVAPPSV LENIQSNCHG PIFMDFAINK LKKAGNQTGF 430 440 450 460 470 480 YVLRCSPKDF KKYFLTFAIE RENATDYKHC LITKNENGEY NLSGTKRSFG NLKDLLTCYQ 490 500 510 520 530 540 TETVRSDSII FQFIKCCPPK PKDKSNLLVF RSNSVSDVPS SPTLQRHNVS QMVFHKIRNE 550 560 570 580 590 600 DLIFEESLGQ GTFTKIFKGI RKEVGDYGQL HQTEVLLKVL DKVHRNYSES FFEAASMMSQ 610 620 630 640 650 660 LSYKHLVLNY GVCVCGEENI LVQEYVKFGS LDTYLKKNKN VINILWKLEV AKQLALAMHF 670 680 690 700 710 720 LEDKGLVHGN VCAKNILLIR EEDRKSGNLP FIKLSDPGIS ITVLPRDILL ERIPWVPPEC 730 740 750 760 770 780 IENPRQLSLA TDKWSFGTTL WEICSGGDKP LSALDSSRKL QFYEDRHQLP APNWTELANL 790 800 810 820 830 840 INNCMDYEPD FRPSFRAIIR DLNSLFTPDY ELLTENDMLP NIRTGALGFS GAFEDRDPTQ 850 860 870 880 890 900 FEERHLKFLQ QLGKGNFGSV EMCRYDPLQD NTGEVVAVKK LQHSTEEHLR DFEREIEILK 910 920 930 940 950 960 SLQHDNIVKY KGVCYSAGRR NLRLIMEYLP YGSLRDYLQK HKERLDHKKL LLYASQICKG 970 980 990 1000 1010 1020 MEYLGTKRYV HRDLATRNIL VENENRVKIG DFGLTKVLPQ DKEYYKVKEP GESPIFWYAP 1030 1040 1050 1060 1070 1080 ESLTESKFSV ASDVWSFGVV LYELFTYIEK SKSPPAEFMR MIGNDKQGQM IVFHLIELLK 1090 1100 1110 1120 NNGRLPRPDG CPDEIYAIMK ECWNNNVAQR PTFRDLAQRV DQIRDNMGG

References

[1]	"molecular cloning and characterization of chicken tyrosine kinase JAK-2." Sayed A.A., Horiuchi H., Furusawa S., Matsuda H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB159042 mRNA. Translation: BAD07298.1.
IPI	IPI00584490.
RefSeq	NP_001025709.1.
UniGene	Gga.954
3D structure databases
HSSP	HSSP built from PDB template 2B7A based on UniProtKB O60674.
SMR	Q75R65. Positions 395-808, 837-1129.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75R65.
Genome annotation databases
Ensembl	ENSGALT00000024235; ENSGALP00000024189; ENSGALG00000015027; Gallus gallus. [Genome view]
GeneID	374199.
KEGG	gga:374199.
Organism-specific databases
CTD	374199.
Phylogenomic databases
HOVERGEN	Q75R65.
Enzyme and pathway databases
BRENDA	2.7.10.2. 4.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR000299. FERM_domain. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2. IPR020693. Tyr_kinase_non-rcpt_Jak2. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR009129. Tyr_prot_kinase_JAK2_cons-reg. IPR009127. Tyr_prot_kinase_JAK_cons-reg. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF49. Tyr_kinase_non-rcpt_Jak2. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00017. SH2. 1 hit. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01825. JANUSKINASE2.
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JAK2_CHICK

Accession

Primary (citable) accession number: Q75R65

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)