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Q75QN6

- DPHS1_ARATH

UniProt

Q75QN6 - DPHS1_ARATH

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Protein
Dual specificity protein phosphatase PHS1
Gene
PHS1, At5g23720, MQM1.1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable dual specificity phosphatase that binds and dephosphorylates MPK18, modulating the organization and dynamics of cortical microtubules. Acts as negative regulator of abscisic acid (ABA) signaling during seed germination and light-induced stomata aperture.3 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei792 – 7921Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. MAP kinase phosphatase activity Source: RefGenome
  2. phosphoprotein phosphatase activity Source: TAIR
  3. phosphotransferase activity, alcohol group as acceptor Source: InterPro
  4. protein binding Source: IntAct
  5. protein tyrosine phosphatase activity Source: UniProtKB-EC
  6. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. cortical microtubule organization Source: TAIR
  3. inactivation of MAPK activity Source: GOC
  4. protein dephosphorylation Source: RefGenome
  5. regulation of MAP kinase activity Source: RefGenome
  6. regulation of gene expression Source: TAIR
  7. regulation of stomatal movement Source: TAIR
  8. response to abscisic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Enzyme and pathway databases

BioCyciARA:AT5G23720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase PHS1 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Protein PROPYZAMIDE-HYPERSENSITIVE 1
Gene namesi
Name:PHS1
Ordered Locus Names:At5g23720
ORF Names:MQM1.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G23720.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous in the phs1-2 allele (1 Publication), but after several backcrossing, phs1-2 and other homozygous alleles (phs1-4 and phs1-5) are similar to the wild-type (1 Publication).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi64 – 641R → C in phs1-1; reduces activity 2-fold. Primary roots skewing. No effect on the interaction with MPK18. 2 Publications
Mutagenesisi792 – 7921C → S: Loss of activity. 1 Publication
Mutagenesisi906 – 9061L → A: Loss of nuclear export; when associated with A-909. 1 Publication
Mutagenesisi909 – 9091L → A: Loss of nuclear export; when associated with A-906. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Dual specificity protein phosphatase PHS1
PRO_0000417331Add
BLAST

Proteomic databases

PaxDbiQ75QN6.
PRIDEiQ75QN6.

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.2 Publications

Inductioni

By abscisic acid.1 Publication

Gene expression databases

GenevestigatoriQ75QN6.

Interactioni

Subunit structurei

Interacts with MPK18.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK18Q9C5C03EBI-2349366,EBI-1238534

Protein-protein interaction databases

IntActiQ75QN6. 2 interactions.
STRINGi3702.AT5G23720.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ75QN6.
SMRiQ75QN6. Positions 711-845.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini703 – 847145Tyrosine-protein phosphatase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni792 – 7987Substrate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi903 – 9119Nuclear export signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000030291.
InParanoidiQ75QN6.
OMAiPNDGFAR.
PhylomeDBiQ75QN6.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
3.90.190.10. 1 hit.
InterProiIPR015275. Actin-fragmin_kin_cat_dom.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF09192. Act-Frag_cataly. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q75QN6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEPEKKRDQ PFSQEKDEEK DLYLVHDEHE SPLPLTVTSR VLYMLGDIAS    50
GPAYRFTQWL DLVRKRSATY GSSGFPHRLH RIDDMVTSAG ERNTDPKSPP 100
SRQSSEISLW ERLGKASTVD IDSSCFSWNM LSSLHHTEHS SSTDHSEEDQ 150
SKPLEVTVNS GGVVFFALFN SSSSEDASRK EEAAVIKFAS SRMATQSERL 200
GYEFSKWLGV QIPQARVIHS CNPEWTLIKE ATEKAQAKAT SEGDEVGEMT 250
CSELLEALEL SRCLLLMSYV HGCPMLESMS SFETEEKAER AAAALGRILV 300
LDLVIRNEDR LPCRQLRWRG NPANLLLTDR IVSSAKHHEC SFDEAFDSAI 350
KRYHPKDYRS IQRERRASSV DSRSRLSISD QMLVSQASDF SDITESPRSY 400
DTGLMSPMSD RSVAADFHLV AIDSGVPRRP PAGKRASDQE IYPRLVELLL 450
NSSQYSSNLL HEITEGSLGY PQAEDGEETS NVRSVVTPVV REFRNGFRAG 500
LRDLQEFHIF LVTLHQKLDV LLRAFFSMMD KTMCADFDRE DFAVPESPSH 550
THGHEVNHYP SPSKDRVPSD NSSDHSESDM QKSVPRTPNS ENKEDGSSPK 600
SRESWHGRSG KGGESLSSQR LAAKLRDFHK FAKVDAESNK ELDQWNETLR 650
NEVMKLCQEN GFNTGFFEGS DNNSCTDAYE LKVRLEHILE RISLISKAAN 700
TEKPSMIQEN LFIGGGLAAR SIYTLQHLGI THVLCLCANE IGQSDTQYPD 750
LFEYQNFSIT DDEDSNIESI FQEALDFIKH GEETGGKILV HCFEGRSRSA 800
TVVLAYLMLQ KKLTLLEAWS KLRKVHRRAQ PNDGFARILI NLDKKCHGKV 850
SMEWRQRKPT MKVCPVCGKN AGLSSSSLKL HLQKSHRKLS SGSVDSAMNM 900
EIQKALEALK LSTGRGSSAS SNSFQSHPG 929
Length:929
Mass (Da):104,235
Last modified:July 5, 2004 - v1
Checksum:i5FE10C006DD39A07
GO
Isoform 2 (identifier: Q75QN6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: MAEPEKKRDQ...HRIDDMVTSA → MIWSL

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:845
Mass (Da):94,591
Checksum:iFDC18FA704D11838
GO

Sequence cautioni

The sequence BAB10045.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8989MAEPE…MVTSA → MIWSL in isoform 2.
VSP_043502Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB161693 mRNA. Translation: BAD18373.1.
AB005244, AB025633 Genomic DNA. Translation: BAB10045.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93202.1.
CP002688 Genomic DNA. Translation: AED93203.1.
RefSeqiNP_197761.2. NM_122277.2. [Q75QN6-2]
NP_851066.2. NM_180735.3. [Q75QN6-1]
UniGeneiAt.22786.

Genome annotation databases

EnsemblPlantsiAT5G23720.1; AT5G23720.1; AT5G23720. [Q75QN6-1]
GeneIDi832437.
KEGGiath:AT5G23720.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB161693 mRNA. Translation: BAD18373.1 .
AB005244 , AB025633 Genomic DNA. Translation: BAB10045.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED93202.1 .
CP002688 Genomic DNA. Translation: AED93203.1 .
RefSeqi NP_197761.2. NM_122277.2. [Q75QN6-2 ]
NP_851066.2. NM_180735.3. [Q75QN6-1 ]
UniGenei At.22786.

3D structure databases

ProteinModelPortali Q75QN6.
SMRi Q75QN6. Positions 711-845.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q75QN6. 2 interactions.
STRINGi 3702.AT5G23720.1-P.

Proteomic databases

PaxDbi Q75QN6.
PRIDEi Q75QN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G23720.1 ; AT5G23720.1 ; AT5G23720 . [Q75QN6-1 ]
GeneIDi 832437.
KEGGi ath:AT5G23720.

Organism-specific databases

TAIRi AT5G23720.

Phylogenomic databases

eggNOGi COG2453.
HOGENOMi HOG000030291.
InParanoidi Q75QN6.
OMAi PNDGFAR.
PhylomeDBi Q75QN6.

Enzyme and pathway databases

BioCyci ARA:AT5G23720-MONOMER.

Gene expression databases

Genevestigatori Q75QN6.

Family and domain databases

Gene3Di 1.10.1070.11. 2 hits.
3.90.190.10. 1 hit.
InterProi IPR015275. Actin-fragmin_kin_cat_dom.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR015880. Znf_C2H2-like.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF09192. Act-Frag_cataly. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A semidominant mutation in an Arabidopsis mitogen-activated protein kinase phosphatase-like gene compromises cortical microtubule organization."
    Naoi K., Hashimoto T.
    Plant Cell 16:1841-1853(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-64 AND CYS-792.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "The phs1-3 mutation in a putative dual-specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana."
    Quettier A.L., Bertrand C., Habricot Y., Miginiac E., Agnes C., Jeannette E., Maldiney R.
    Plant J. 47:711-719(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY ABSCISIC ACID.
  6. "Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells."
    Walia A., Lee J.S., Wasteneys G., Ellis B.
    Plant J. 59:565-575(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPK18, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-64.
  7. "Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms."
    Pytela J., Kato T., Hashimoto T.
    Planta 231:1311-1322(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-906 AND LEU-909.

Entry informationi

Entry nameiDPHS1_ARATH
AccessioniPrimary (citable) accession number: Q75QN6
Secondary accession number(s): F4KE97, Q9FFA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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