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Protein

Dual specificity protein phosphatase PHS1

Gene

PHS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable dual specificity phosphatase that binds and dephosphorylates MPK18, modulating the organization and dynamics of cortical microtubules. Acts as negative regulator of abscisic acid (ABA) signaling during seed germination and light-induced stomata aperture.3 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei792Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • cortical microtubule organization Source: TAIR
  • regulation of gene expression Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to abscisic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Enzyme and pathway databases

ReactomeiR-ATH-202670. ERKs are inactivated.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase PHS1 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Protein PROPYZAMIDE-HYPERSENSITIVE 1
Gene namesi
Name:PHS1
Ordered Locus Names:At5g23720
ORF Names:MQM1.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23720.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous in the phs1-2 allele (PubMed:15208393), but after several backcrossing, phs1-2 and other homozygous alleles (phs1-4 and phs1-5) are similar to the wild-type (PubMed:20224945).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64R → C in phs1-1; reduces activity 2-fold. Primary roots skewing. No effect on the interaction with MPK18. 2 Publications1
Mutagenesisi792C → S: Loss of activity. 1 Publication1
Mutagenesisi906L → A: Loss of nuclear export; when associated with A-909. 1 Publication1
Mutagenesisi909L → A: Loss of nuclear export; when associated with A-906. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173311 – 929Dual specificity protein phosphatase PHS1Add BLAST929

Proteomic databases

PaxDbiQ75QN6.
PRIDEiQ75QN6.

PTM databases

iPTMnetiQ75QN6.

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.2 Publications

Inductioni

By abscisic acid.1 Publication

Gene expression databases

ExpressionAtlasiQ75QN6. baseline and differential.
GenevisibleiQ75QN6. AT.

Interactioni

Subunit structurei

Interacts with MPK18.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK18Q9C5C03EBI-2349366,EBI-1238534

Protein-protein interaction databases

BioGridi17712. 5 interactors.
IntActiQ75QN6. 2 interactors.
STRINGi3702.AT5G23720.1.

Structurei

3D structure databases

ProteinModelPortaliQ75QN6.
SMRiQ75QN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini703 – 847Tyrosine-protein phosphataseAdd BLAST145

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni792 – 798Substrate bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi903 – 911Nuclear export signalSequence analysis9

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
HOGENOMiHOG000030291.
KOiK14165.
OMAiSEISLWE.
OrthoDBiEOG093602JP.
PhylomeDBiQ75QN6.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di1.10.1070.11. 2 hits.
3.90.190.10. 1 hit.
InterProiIPR015275. Actin-fragmin_kin_cat_dom.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF09192. Act-Frag_cataly. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q75QN6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEPEKKRDQ PFSQEKDEEK DLYLVHDEHE SPLPLTVTSR VLYMLGDIAS
60 70 80 90 100
GPAYRFTQWL DLVRKRSATY GSSGFPHRLH RIDDMVTSAG ERNTDPKSPP
110 120 130 140 150
SRQSSEISLW ERLGKASTVD IDSSCFSWNM LSSLHHTEHS SSTDHSEEDQ
160 170 180 190 200
SKPLEVTVNS GGVVFFALFN SSSSEDASRK EEAAVIKFAS SRMATQSERL
210 220 230 240 250
GYEFSKWLGV QIPQARVIHS CNPEWTLIKE ATEKAQAKAT SEGDEVGEMT
260 270 280 290 300
CSELLEALEL SRCLLLMSYV HGCPMLESMS SFETEEKAER AAAALGRILV
310 320 330 340 350
LDLVIRNEDR LPCRQLRWRG NPANLLLTDR IVSSAKHHEC SFDEAFDSAI
360 370 380 390 400
KRYHPKDYRS IQRERRASSV DSRSRLSISD QMLVSQASDF SDITESPRSY
410 420 430 440 450
DTGLMSPMSD RSVAADFHLV AIDSGVPRRP PAGKRASDQE IYPRLVELLL
460 470 480 490 500
NSSQYSSNLL HEITEGSLGY PQAEDGEETS NVRSVVTPVV REFRNGFRAG
510 520 530 540 550
LRDLQEFHIF LVTLHQKLDV LLRAFFSMMD KTMCADFDRE DFAVPESPSH
560 570 580 590 600
THGHEVNHYP SPSKDRVPSD NSSDHSESDM QKSVPRTPNS ENKEDGSSPK
610 620 630 640 650
SRESWHGRSG KGGESLSSQR LAAKLRDFHK FAKVDAESNK ELDQWNETLR
660 670 680 690 700
NEVMKLCQEN GFNTGFFEGS DNNSCTDAYE LKVRLEHILE RISLISKAAN
710 720 730 740 750
TEKPSMIQEN LFIGGGLAAR SIYTLQHLGI THVLCLCANE IGQSDTQYPD
760 770 780 790 800
LFEYQNFSIT DDEDSNIESI FQEALDFIKH GEETGGKILV HCFEGRSRSA
810 820 830 840 850
TVVLAYLMLQ KKLTLLEAWS KLRKVHRRAQ PNDGFARILI NLDKKCHGKV
860 870 880 890 900
SMEWRQRKPT MKVCPVCGKN AGLSSSSLKL HLQKSHRKLS SGSVDSAMNM
910 920
EIQKALEALK LSTGRGSSAS SNSFQSHPG
Length:929
Mass (Da):104,235
Last modified:July 5, 2004 - v1
Checksum:i5FE10C006DD39A07
GO
Isoform 2 (identifier: Q75QN6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: MAEPEKKRDQ...HRIDDMVTSA → MIWSL

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:845
Mass (Da):94,591
Checksum:iFDC18FA704D11838
GO

Sequence cautioni

The sequence BAB10045 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0435021 – 89MAEPE…MVTSA → MIWSL in isoform 2. CuratedAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161693 mRNA. Translation: BAD18373.1.
AB005244, AB025633 Genomic DNA. Translation: BAB10045.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93202.1.
CP002688 Genomic DNA. Translation: AED93203.1.
RefSeqiNP_197761.2. NM_122277.2. [Q75QN6-2]
NP_851066.2. NM_180735.4. [Q75QN6-1]
UniGeneiAt.22786.

Genome annotation databases

EnsemblPlantsiAT5G23720.1; AT5G23720.1; AT5G23720. [Q75QN6-1]
GeneIDi832437.
GrameneiAT5G23720.1; AT5G23720.1; AT5G23720.
KEGGiath:AT5G23720.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161693 mRNA. Translation: BAD18373.1.
AB005244, AB025633 Genomic DNA. Translation: BAB10045.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93202.1.
CP002688 Genomic DNA. Translation: AED93203.1.
RefSeqiNP_197761.2. NM_122277.2. [Q75QN6-2]
NP_851066.2. NM_180735.4. [Q75QN6-1]
UniGeneiAt.22786.

3D structure databases

ProteinModelPortaliQ75QN6.
SMRiQ75QN6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17712. 5 interactors.
IntActiQ75QN6. 2 interactors.
STRINGi3702.AT5G23720.1.

PTM databases

iPTMnetiQ75QN6.

Proteomic databases

PaxDbiQ75QN6.
PRIDEiQ75QN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23720.1; AT5G23720.1; AT5G23720. [Q75QN6-1]
GeneIDi832437.
GrameneiAT5G23720.1; AT5G23720.1; AT5G23720.
KEGGiath:AT5G23720.

Organism-specific databases

TAIRiAT5G23720.

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
HOGENOMiHOG000030291.
KOiK14165.
OMAiSEISLWE.
OrthoDBiEOG093602JP.
PhylomeDBiQ75QN6.

Enzyme and pathway databases

ReactomeiR-ATH-202670. ERKs are inactivated.

Miscellaneous databases

PROiQ75QN6.

Gene expression databases

ExpressionAtlasiQ75QN6. baseline and differential.
GenevisibleiQ75QN6. AT.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di1.10.1070.11. 2 hits.
3.90.190.10. 1 hit.
InterProiIPR015275. Actin-fragmin_kin_cat_dom.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF09192. Act-Frag_cataly. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPHS1_ARATH
AccessioniPrimary (citable) accession number: Q75QN6
Secondary accession number(s): F4KE97, Q9FFA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.