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Q75QN6

- DPHS1_ARATH

UniProt

Q75QN6 - DPHS1_ARATH

Protein

Dual specificity protein phosphatase PHS1

Gene

PHS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Probable dual specificity phosphatase that binds and dephosphorylates MPK18, modulating the organization and dynamics of cortical microtubules. Acts as negative regulator of abscisic acid (ABA) signaling during seed germination and light-induced stomata aperture.3 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei792 – 7921Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase phosphatase activity Source: RefGenome
    2. phosphoprotein phosphatase activity Source: TAIR
    3. phosphotransferase activity, alcohol group as acceptor Source: InterPro
    4. protein binding Source: IntAct
    5. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    6. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
    2. cortical microtubule organization Source: TAIR
    3. inactivation of MAPK activity Source: GOC
    4. protein dephosphorylation Source: RefGenome
    5. regulation of gene expression Source: TAIR
    6. regulation of MAP kinase activity Source: RefGenome
    7. regulation of stomatal movement Source: TAIR
    8. response to abscisic acid Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Abscisic acid signaling pathway

    Enzyme and pathway databases

    BioCyciARA:AT5G23720-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase PHS1 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    Protein PROPYZAMIDE-HYPERSENSITIVE 1
    Gene namesi
    Name:PHS1
    Ordered Locus Names:At5g23720
    ORF Names:MQM1.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G23720.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethality when homozygous in the phs1-2 allele (PubMed:15208393), but after several backcrossing, phs1-2 and other homozygous alleles (phs1-4 and phs1-5) are similar to the wild-type (PubMed:20224945).2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi64 – 641R → C in phs1-1; reduces activity 2-fold. Primary roots skewing. No effect on the interaction with MPK18. 2 Publications
    Mutagenesisi792 – 7921C → S: Loss of activity. 1 Publication
    Mutagenesisi906 – 9061L → A: Loss of nuclear export; when associated with A-909. 1 Publication
    Mutagenesisi909 – 9091L → A: Loss of nuclear export; when associated with A-906. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Dual specificity protein phosphatase PHS1PRO_0000417331Add
    BLAST

    Proteomic databases

    PaxDbiQ75QN6.
    PRIDEiQ75QN6.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves and flowers.2 Publications

    Inductioni

    By abscisic acid.1 Publication

    Gene expression databases

    GenevestigatoriQ75QN6.

    Interactioni

    Subunit structurei

    Interacts with MPK18.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MPK18Q9C5C03EBI-2349366,EBI-1238534

    Protein-protein interaction databases

    IntActiQ75QN6. 2 interactions.
    STRINGi3702.AT5G23720.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75QN6.
    SMRiQ75QN6. Positions 711-845.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini703 – 847145Tyrosine-protein phosphataseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni792 – 7987Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi903 – 9119Nuclear export signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2453.
    HOGENOMiHOG000030291.
    InParanoidiQ75QN6.
    OMAiPNDGFAR.
    PhylomeDBiQ75QN6.

    Family and domain databases

    Gene3Di1.10.1070.11. 2 hits.
    3.90.190.10. 1 hit.
    InterProiIPR015275. Actin-fragmin_kin_cat_dom.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF09192. Act-Frag_cataly. 1 hit.
    PF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q75QN6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEPEKKRDQ PFSQEKDEEK DLYLVHDEHE SPLPLTVTSR VLYMLGDIAS    50
    GPAYRFTQWL DLVRKRSATY GSSGFPHRLH RIDDMVTSAG ERNTDPKSPP 100
    SRQSSEISLW ERLGKASTVD IDSSCFSWNM LSSLHHTEHS SSTDHSEEDQ 150
    SKPLEVTVNS GGVVFFALFN SSSSEDASRK EEAAVIKFAS SRMATQSERL 200
    GYEFSKWLGV QIPQARVIHS CNPEWTLIKE ATEKAQAKAT SEGDEVGEMT 250
    CSELLEALEL SRCLLLMSYV HGCPMLESMS SFETEEKAER AAAALGRILV 300
    LDLVIRNEDR LPCRQLRWRG NPANLLLTDR IVSSAKHHEC SFDEAFDSAI 350
    KRYHPKDYRS IQRERRASSV DSRSRLSISD QMLVSQASDF SDITESPRSY 400
    DTGLMSPMSD RSVAADFHLV AIDSGVPRRP PAGKRASDQE IYPRLVELLL 450
    NSSQYSSNLL HEITEGSLGY PQAEDGEETS NVRSVVTPVV REFRNGFRAG 500
    LRDLQEFHIF LVTLHQKLDV LLRAFFSMMD KTMCADFDRE DFAVPESPSH 550
    THGHEVNHYP SPSKDRVPSD NSSDHSESDM QKSVPRTPNS ENKEDGSSPK 600
    SRESWHGRSG KGGESLSSQR LAAKLRDFHK FAKVDAESNK ELDQWNETLR 650
    NEVMKLCQEN GFNTGFFEGS DNNSCTDAYE LKVRLEHILE RISLISKAAN 700
    TEKPSMIQEN LFIGGGLAAR SIYTLQHLGI THVLCLCANE IGQSDTQYPD 750
    LFEYQNFSIT DDEDSNIESI FQEALDFIKH GEETGGKILV HCFEGRSRSA 800
    TVVLAYLMLQ KKLTLLEAWS KLRKVHRRAQ PNDGFARILI NLDKKCHGKV 850
    SMEWRQRKPT MKVCPVCGKN AGLSSSSLKL HLQKSHRKLS SGSVDSAMNM 900
    EIQKALEALK LSTGRGSSAS SNSFQSHPG 929
    Length:929
    Mass (Da):104,235
    Last modified:July 5, 2004 - v1
    Checksum:i5FE10C006DD39A07
    GO
    Isoform 2 (identifier: Q75QN6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-89: MAEPEKKRDQ...HRIDDMVTSA → MIWSL

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:845
    Mass (Da):94,591
    Checksum:iFDC18FA704D11838
    GO

    Sequence cautioni

    The sequence BAB10045.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8989MAEPE…MVTSA → MIWSL in isoform 2. CuratedVSP_043502Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161693 mRNA. Translation: BAD18373.1.
    AB005244, AB025633 Genomic DNA. Translation: BAB10045.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED93202.1.
    CP002688 Genomic DNA. Translation: AED93203.1.
    RefSeqiNP_197761.2. NM_122277.2. [Q75QN6-2]
    NP_851066.2. NM_180735.3. [Q75QN6-1]
    UniGeneiAt.22786.

    Genome annotation databases

    EnsemblPlantsiAT5G23720.1; AT5G23720.1; AT5G23720. [Q75QN6-1]
    GeneIDi832437.
    KEGGiath:AT5G23720.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161693 mRNA. Translation: BAD18373.1 .
    AB005244 , AB025633 Genomic DNA. Translation: BAB10045.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED93202.1 .
    CP002688 Genomic DNA. Translation: AED93203.1 .
    RefSeqi NP_197761.2. NM_122277.2. [Q75QN6-2 ]
    NP_851066.2. NM_180735.3. [Q75QN6-1 ]
    UniGenei At.22786.

    3D structure databases

    ProteinModelPortali Q75QN6.
    SMRi Q75QN6. Positions 711-845.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q75QN6. 2 interactions.
    STRINGi 3702.AT5G23720.1-P.

    Proteomic databases

    PaxDbi Q75QN6.
    PRIDEi Q75QN6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G23720.1 ; AT5G23720.1 ; AT5G23720 . [Q75QN6-1 ]
    GeneIDi 832437.
    KEGGi ath:AT5G23720.

    Organism-specific databases

    TAIRi AT5G23720.

    Phylogenomic databases

    eggNOGi COG2453.
    HOGENOMi HOG000030291.
    InParanoidi Q75QN6.
    OMAi PNDGFAR.
    PhylomeDBi Q75QN6.

    Enzyme and pathway databases

    BioCyci ARA:AT5G23720-MONOMER.

    Gene expression databases

    Genevestigatori Q75QN6.

    Family and domain databases

    Gene3Di 1.10.1070.11. 2 hits.
    3.90.190.10. 1 hit.
    InterProi IPR015275. Actin-fragmin_kin_cat_dom.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF09192. Act-Frag_cataly. 1 hit.
    PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A semidominant mutation in an Arabidopsis mitogen-activated protein kinase phosphatase-like gene compromises cortical microtubule organization."
      Naoi K., Hashimoto T.
      Plant Cell 16:1841-1853(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-64 AND CYS-792.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "The phs1-3 mutation in a putative dual-specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana."
      Quettier A.L., Bertrand C., Habricot Y., Miginiac E., Agnes C., Jeannette E., Maldiney R.
      Plant J. 47:711-719(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY ABSCISIC ACID.
    6. "Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells."
      Walia A., Lee J.S., Wasteneys G., Ellis B.
      Plant J. 59:565-575(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPK18, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-64.
    7. "Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms."
      Pytela J., Kato T., Hashimoto T.
      Planta 231:1311-1322(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-906 AND LEU-909.

    Entry informationi

    Entry nameiDPHS1_ARATH
    AccessioniPrimary (citable) accession number: Q75QN6
    Secondary accession number(s): F4KE97, Q9FFA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3