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Q75QN2 (INT8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrator complex subunit 8

Short name=Int8
Alternative name(s):
Protein kaonashi-1
Gene names
Name:INTS8
Synonyms:C8orf52
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length995 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Subunit structure

Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Ref.6

Subcellular location

Nucleus Probable.

Sequence similarities

Contains 4 TPR repeats.

Sequence caution

The sequence AAH50536.1 differs from that shown. Reason: Frameshift at position 985.

The sequence BAA91238.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA91671.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
TPR repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsnRNA processing

Inferred from direct assay Ref.6. Source: HGNC

   Cellular_componentintegrator complex

Inferred from direct assay Ref.6. Source: HGNC

   Molecular_functionprotein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q75QN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q75QN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     879-895: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 995995Integrator complex subunit 8
PRO_0000259553

Regions

Repeat250 – 28839TPR 1
Repeat320 – 35637TPR 2
Repeat570 – 60334TPR 3
Repeat833 – 86634TPR 4

Amino acid modifications

Modified residue181Phosphothreonine Ref.7

Natural variations

Alternative sequence879 – 89517Missing in isoform 2.
VSP_021469

Experimental info

Sequence conflict7781V → A in BAA91238. Ref.5
Sequence conflict8321N → S in CAD98067. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 24D06E11821BD4D5

FASTA995113,088
        10         20         30         40         50         60 
MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK 

        70         80         90        100        110        120 
PSVNEQNQVQ PPPDNKRNRI LKLLALKVAA HLKWDLDILE KSLSVPVLNM LLNELLCISK 

       130        140        150        160        170        180 
VPPGTKHVDM DLATLPPTTA MAVLLYNRWA IRTIVQSSFP VKQAKPGPPQ LSVMNQMQQE 

       190        200        210        220        230        240 
KELTENILKV LKEQAADSIL VLEAALKLNK DLYVHTMRTL DLLAMEPGMV NGETESSTAG 

       250        260        270        280        290        300 
LKVKTEEMQC QVCYDLGAAY FQQGSTNSAV YENAREKFFR TKELIAEIGS LSLHCTIDEK 

       310        320        330        340        350        360 
RLAGYCQACD VLVPSSDSTS QQLTPYSQVH ICLRSGNYQE VIQIFIEDNL TLSLPVQFRQ 

       370        380        390        400        410        420 
SVLRELFKKA QQGNEALDEI CFKVCACNTV RDILEGRTIS VQFNQLFLRP NKEKIDFLLE 

       430        440        450        460        470        480 
VCSRSVNLEK ASESLKGNMA AFLKNVCLGL EDLQYVFMIS SHELFITLLK DEERKLLVDQ 

       490        500        510        520        530        540 
MRKRSPRVNL CIKPVTSFYD IPASASVNIG QLEHQLILSV DPWRIRQILI ELHGMTSERQ 

       550        560        570        580        590        600 
FWTVSNKWEV PSVYSGVILG IKDNLTRDLV YILMAKGLHC STVKDFSHAK QLFAACLELV 

       610        620        630        640        650        660 
TEFSPKLRQV MLNEMLLLDI HTHEAGTGQA GERPPSDLIS RVRGYLEMRL PDIPLRQVIA 

       670        680        690        700        710        720 
EECVAFMLNW RENEYLTLQV PAFLLQSNPY VKLGQLLAAT CKELPGPKES RRTAKDLWEV 

       730        740        750        760        770        780 
VVQICSVSSQ HKRGNDGRVS LIKQRESTLG IMYRSELLSF IKKLREPLVL TIILSLFVKL 

       790        800        810        820        830        840 
HNVREDIVND ITAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA 

       850        860        870        880        890        900 
DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCSL LNCHTQVAIL 

       910        920        930        940        950        960 
CQFLREIDYK TAFKSLQEQN SHDAMDSYYD YIWDVTILEY LTYLHHKRGE TDKRQIAIKA 

       970        980        990 
IGQTELNASN PEEVLQLAAQ RRKKKFLQAM AKLYF 

« Hide

Isoform 2 [UniParc].

Checksum: A1068A253A9BD8C2
Show »

FASTA978111,116

References

« Hide 'large scale' references
[1]"Novel gene with no significant domain."
Atsushi S., Asakawa S., Shimizu N.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Testis and Uterus.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-995 (ISOFORM 2).
Tissue: Rectum tumor.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 307-995 (ISOFORM 1).
Tissue: Carcinoma and Teratocarcinoma.
[6]"Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II."
Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N., Shiekhattar R.
Cell 123:265-276(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR COMPLEX.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB161944 mRNA. Translation: BAD10863.1.
CH471060 Genomic DNA. Translation: EAW91726.1.
BC050536 mRNA. Translation: AAH50536.1. Frameshift.
BC064915 mRNA. Translation: AAH64915.1.
BC136754 mRNA. Translation: AAI36755.1.
BX538203 mRNA. Translation: CAD98067.1.
AK000537 mRNA. Translation: BAA91238.1. Different initiation.
AK001403 mRNA. Translation: BAA91671.1. Different initiation.
BK005731 mRNA. Translation: DAA05731.1.
CCDSCCDS34925.1. [Q75QN2-1]
RefSeqNP_060334.2. NM_017864.3. [Q75QN2-1]
UniGeneHs.727669.

3D structure databases

ProteinModelPortalQ75QN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120788. 10 interactions.
IntActQ75QN2. 2 interactions.
MINTMINT-6777569.
STRING9606.ENSP00000343274.

PTM databases

PhosphoSiteQ75QN2.

Polymorphism databases

DMDM74712892.

Proteomic databases

MaxQBQ75QN2.
PaxDbQ75QN2.
PRIDEQ75QN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000447247; ENSP00000398203; ENSG00000164941. [Q75QN2-2]
ENST00000523731; ENSP00000430338; ENSG00000164941. [Q75QN2-1]
GeneID55656.
KEGGhsa:55656.
UCSCuc003yha.2. human. [Q75QN2-1]
uc010mba.3. human. [Q75QN2-2]

Organism-specific databases

CTD55656.
GeneCardsGC08P095825.
HGNCHGNC:26048. INTS8.
HPAHPA057299.
MIM611351. gene.
neXtProtNX_Q75QN2.
PharmGKBPA142672371.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG82988.
HOVERGENHBG080418.
InParanoidQ75QN2.
KOK13145.
OMAWPSSIPN.
OrthoDBEOG77T13W.
PhylomeDBQ75QN2.
TreeFamTF324241.

Gene expression databases

ArrayExpressQ75QN2.
BgeeQ75QN2.
CleanExHS_INTS8.
GenevestigatorQ75QN2.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSIntS8. human.
GeneWikiINTS8.
GenomeRNAi55656.
NextBio60375.
PROQ75QN2.
SOURCESearch...

Entry information

Entry nameINT8_HUMAN
AccessionPrimary (citable) accession number: Q75QN2
Secondary accession number(s): B2RN92 expand/collapse secondary AC list , Q5RKZ3, Q6P1R5, Q7Z314, Q9NVS6, Q9NWY7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM