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Q75QN2

- INT8_HUMAN

UniProt

Q75QN2 - INT8_HUMAN

Protein

Integrator complex subunit 8

Gene

INTS8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. snRNA processing Source: HGNC

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrator complex subunit 8
    Short name:
    Int8
    Alternative name(s):
    Protein kaonashi-1
    Gene namesi
    Name:INTS8
    Synonyms:C8orf52
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:26048. INTS8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. integrator complex Source: HGNC

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142672371.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 995995Integrator complex subunit 8PRO_0000259553Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ75QN2.
    PaxDbiQ75QN2.
    PRIDEiQ75QN2.

    PTM databases

    PhosphoSiteiQ75QN2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ75QN2.
    BgeeiQ75QN2.
    CleanExiHS_INTS8.
    GenevestigatoriQ75QN2.

    Organism-specific databases

    HPAiHPA057299.

    Interactioni

    Subunit structurei

    Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12.1 Publication

    Protein-protein interaction databases

    BioGridi120788. 10 interactions.
    IntActiQ75QN2. 2 interactions.
    MINTiMINT-6777569.
    STRINGi9606.ENSP00000343274.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75QN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati250 – 28839TPR 1Add
    BLAST
    Repeati320 – 35637TPR 2Add
    BLAST
    Repeati570 – 60334TPR 3Add
    BLAST
    Repeati833 – 86634TPR 4Add
    BLAST

    Sequence similaritiesi

    Contains 4 TPR repeats.Curated

    Keywords - Domaini

    Repeat, TPR repeat

    Phylogenomic databases

    eggNOGiNOG82988.
    HOVERGENiHBG080418.
    InParanoidiQ75QN2.
    KOiK13145.
    OMAiWPSSIPN.
    OrthoDBiEOG77T13W.
    PhylomeDBiQ75QN2.
    TreeFamiTF324241.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q75QN2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL    50
    IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVAA HLKWDLDILE 100
    KSLSVPVLNM LLNELLCISK VPPGTKHVDM DLATLPPTTA MAVLLYNRWA 150
    IRTIVQSSFP VKQAKPGPPQ LSVMNQMQQE KELTENILKV LKEQAADSIL 200
    VLEAALKLNK DLYVHTMRTL DLLAMEPGMV NGETESSTAG LKVKTEEMQC 250
    QVCYDLGAAY FQQGSTNSAV YENAREKFFR TKELIAEIGS LSLHCTIDEK 300
    RLAGYCQACD VLVPSSDSTS QQLTPYSQVH ICLRSGNYQE VIQIFIEDNL 350
    TLSLPVQFRQ SVLRELFKKA QQGNEALDEI CFKVCACNTV RDILEGRTIS 400
    VQFNQLFLRP NKEKIDFLLE VCSRSVNLEK ASESLKGNMA AFLKNVCLGL 450
    EDLQYVFMIS SHELFITLLK DEERKLLVDQ MRKRSPRVNL CIKPVTSFYD 500
    IPASASVNIG QLEHQLILSV DPWRIRQILI ELHGMTSERQ FWTVSNKWEV 550
    PSVYSGVILG IKDNLTRDLV YILMAKGLHC STVKDFSHAK QLFAACLELV 600
    TEFSPKLRQV MLNEMLLLDI HTHEAGTGQA GERPPSDLIS RVRGYLEMRL 650
    PDIPLRQVIA EECVAFMLNW RENEYLTLQV PAFLLQSNPY VKLGQLLAAT 700
    CKELPGPKES RRTAKDLWEV VVQICSVSSQ HKRGNDGRVS LIKQRESTLG 750
    IMYRSELLSF IKKLREPLVL TIILSLFVKL HNVREDIVND ITAEHISIWP 800
    SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS 850
    AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCSL LNCHTQVAIL 900
    CQFLREIDYK TAFKSLQEQN SHDAMDSYYD YIWDVTILEY LTYLHHKRGE 950
    TDKRQIAIKA IGQTELNASN PEEVLQLAAQ RRKKKFLQAM AKLYF 995
    Length:995
    Mass (Da):113,088
    Last modified:July 5, 2004 - v1
    Checksum:i24D06E11821BD4D5
    GO
    Isoform 2 (identifier: Q75QN2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         879-895: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:978
    Mass (Da):111,116
    Checksum:iA1068A253A9BD8C2
    GO

    Sequence cautioni

    The sequence AAH50536.1 differs from that shown. Reason: Frameshift at position 985.
    The sequence BAA91238.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA91671.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti778 – 7781V → A in BAA91238. (PubMed:14702039)Curated
    Sequence conflicti832 – 8321N → S in CAD98067. (PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei879 – 89517Missing in isoform 2. 1 PublicationVSP_021469Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161944 mRNA. Translation: BAD10863.1.
    CH471060 Genomic DNA. Translation: EAW91726.1.
    BC050536 mRNA. Translation: AAH50536.1. Frameshift.
    BC064915 mRNA. Translation: AAH64915.1.
    BC136754 mRNA. Translation: AAI36755.1.
    BX538203 mRNA. Translation: CAD98067.1.
    AK000537 mRNA. Translation: BAA91238.1. Different initiation.
    AK001403 mRNA. Translation: BAA91671.1. Different initiation.
    BK005731 mRNA. Translation: DAA05731.1.
    CCDSiCCDS34925.1. [Q75QN2-1]
    RefSeqiNP_060334.2. NM_017864.3. [Q75QN2-1]
    UniGeneiHs.727669.

    Genome annotation databases

    EnsembliENST00000523731; ENSP00000430338; ENSG00000164941. [Q75QN2-1]
    GeneIDi55656.
    KEGGihsa:55656.
    UCSCiuc003yha.2. human. [Q75QN2-1]
    uc010mba.3. human. [Q75QN2-2]

    Polymorphism databases

    DMDMi74712892.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161944 mRNA. Translation: BAD10863.1 .
    CH471060 Genomic DNA. Translation: EAW91726.1 .
    BC050536 mRNA. Translation: AAH50536.1 . Frameshift.
    BC064915 mRNA. Translation: AAH64915.1 .
    BC136754 mRNA. Translation: AAI36755.1 .
    BX538203 mRNA. Translation: CAD98067.1 .
    AK000537 mRNA. Translation: BAA91238.1 . Different initiation.
    AK001403 mRNA. Translation: BAA91671.1 . Different initiation.
    BK005731 mRNA. Translation: DAA05731.1 .
    CCDSi CCDS34925.1. [Q75QN2-1 ]
    RefSeqi NP_060334.2. NM_017864.3. [Q75QN2-1 ]
    UniGenei Hs.727669.

    3D structure databases

    ProteinModelPortali Q75QN2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120788. 10 interactions.
    IntActi Q75QN2. 2 interactions.
    MINTi MINT-6777569.
    STRINGi 9606.ENSP00000343274.

    PTM databases

    PhosphoSitei Q75QN2.

    Polymorphism databases

    DMDMi 74712892.

    Proteomic databases

    MaxQBi Q75QN2.
    PaxDbi Q75QN2.
    PRIDEi Q75QN2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000523731 ; ENSP00000430338 ; ENSG00000164941 . [Q75QN2-1 ]
    GeneIDi 55656.
    KEGGi hsa:55656.
    UCSCi uc003yha.2. human. [Q75QN2-1 ]
    uc010mba.3. human. [Q75QN2-2 ]

    Organism-specific databases

    CTDi 55656.
    GeneCardsi GC08P095825.
    HGNCi HGNC:26048. INTS8.
    HPAi HPA057299.
    MIMi 611351. gene.
    neXtProti NX_Q75QN2.
    PharmGKBi PA142672371.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG82988.
    HOVERGENi HBG080418.
    InParanoidi Q75QN2.
    KOi K13145.
    OMAi WPSSIPN.
    OrthoDBi EOG77T13W.
    PhylomeDBi Q75QN2.
    TreeFami TF324241.

    Miscellaneous databases

    ChiTaRSi IntS8. human.
    GeneWikii INTS8.
    GenomeRNAii 55656.
    NextBioi 60375.
    PROi Q75QN2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q75QN2.
    Bgeei Q75QN2.
    CleanExi HS_INTS8.
    Genevestigatori Q75QN2.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Novel gene with no significant domain."
      Atsushi S., Asakawa S., Shimizu N.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain, Testis and Uterus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-995 (ISOFORM 2).
      Tissue: Rectum tumor.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 307-995 (ISOFORM 1).
      Tissue: Carcinoma and Teratocarcinoma.
    6. "Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II."
      Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N., Shiekhattar R.
      Cell 123:265-276(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR COMPLEX.
    7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiINT8_HUMAN
    AccessioniPrimary (citable) accession number: Q75QN2
    Secondary accession number(s): B2RN92
    , Q5RKZ3, Q6P1R5, Q7Z314, Q9NVS6, Q9NWY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3