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Q75Q41

- TOM22_RAT

UniProt

Q75Q41 - TOM22_RAT

Protein

Mitochondrial import receptor subunit TOM22 homolog

Gene

Tomm22

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein transmembrane transporter activity Source: BHF-UCL

    GO - Biological processi

    1. protein import into mitochondrial outer membrane Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199211. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import receptor subunit TOM22 homolog
    Short name:
    rTOM22
    Alternative name(s):
    Translocase of outer membrane 22 kDa subunit homolog
    Gene namesi
    Name:Tomm22
    Synonyms:Tom22
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi1303260. Tomm22.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Ensembl
    3. mitochondrial outer membrane translocase complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi42 – 421E → Q: Inhibits localization to the mitochondrion outer membrane. 1 Publication
    Mutagenesisi46 – 461E → P: Inhibits localization to the mitochondrion outer membrane. 1 Publication
    Mutagenesisi49 – 491W → P: Inhibits localization to the mitochondrion outer membrane. 1 Publication
    Mutagenesisi98 – 981P → A: Inhibits localization to the mitochondrion outer membrane. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 142141Mitochondrial import receptor subunit TOM22 homologPRO_0000383359Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei15 – 151PhosphoserineBy similarity
    Modified residuei43 – 431PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ75Q41.
    PRIDEiQ75Q41.

    Expressioni

    Gene expression databases

    GenevestigatoriQ75Q41.

    Interactioni

    Subunit structurei

    Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOMM40 By similarity. Interacts with PPP2R2B.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019323.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 8281CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini104 – 14239Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei83 – 10321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 5010Import sequence; necessary for mitochondrion outer membrane localization and integration in the TOM complex
    Regioni83 – 10321TMD; necessary for mitochondrion outer membrane localization and integration in the TOM complexAdd
    BLAST
    Regioni123 – 14220C-tail signal; necessary for mitochondrion outer membrane localization and integration in the TOM complexAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi110 – 1189Poly-Gln

    Domaini

    The N-terminal domain (residues 1-60) is important for binding to the unfolded mature imported proteins. Residues (47-69) of the cytoplasmic domain interacts with TOMM20 while the C-terminal segment (residues 61-80) binds presequence of preproteins By similarity. Requires the transmembrane domain (TMD), a short segment (the import sequence) in the cytoplasmic domain localizing separately from the TMD and the C-tail signal in the C-terminal domain for efficient targeting and integration into the TOM complex.By similarity

    Sequence similaritiesi

    Belongs to the Tom22 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG310729.
    GeneTreeiENSGT00390000016475.
    HOGENOMiHOG000067816.
    InParanoidiQ75Q41.
    KOiK17769.
    OMAiMMPPAPG.
    OrthoDBiEOG7KWSKG.
    PhylomeDBiQ75Q41.
    TreeFamiTF106201.

    Family and domain databases

    InterProiIPR005683. Tom22.
    [Graphical view]
    PfamiPF04281. Tom22. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q75Q41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAVAAAGA GEPLSPEELV PKAEAEKAEE DLEEDDDDEL DETLSERLWG    50
    LTEMFPERVR SAAGATFDLS LFVAQKMYRF SRAALWIGTT SFMILVLPVV 100
    FETEKLQMEQ QQQLQQRQIL LGPNTGLSGG MPGALPPLPG KI 142
    Length:142
    Mass (Da):15,491
    Last modified:July 5, 2004 - v1
    Checksum:iC1259302C15CA3AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB162854 mRNA. Translation: BAD11364.1.
    CH473950 Genomic DNA. Translation: EDM15790.1.
    BC098639 mRNA. Translation: AAH98639.1.
    RefSeqiNP_997679.1. NM_212514.2.
    UniGeneiRn.3119.

    Genome annotation databases

    EnsembliENSRNOT00000019323; ENSRNOP00000019323; ENSRNOG00000014058.
    GeneIDi300075.
    KEGGirno:300075.
    UCSCiRGD:1303260. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB162854 mRNA. Translation: BAD11364.1 .
    CH473950 Genomic DNA. Translation: EDM15790.1 .
    BC098639 mRNA. Translation: AAH98639.1 .
    RefSeqi NP_997679.1. NM_212514.2.
    UniGenei Rn.3119.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000019323.

    Proteomic databases

    PaxDbi Q75Q41.
    PRIDEi Q75Q41.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019323 ; ENSRNOP00000019323 ; ENSRNOG00000014058 .
    GeneIDi 300075.
    KEGGi rno:300075.
    UCSCi RGD:1303260. rat.

    Organism-specific databases

    CTDi 56993.
    RGDi 1303260. Tomm22.

    Phylogenomic databases

    eggNOGi NOG310729.
    GeneTreei ENSGT00390000016475.
    HOGENOMi HOG000067816.
    InParanoidi Q75Q41.
    KOi K17769.
    OMAi MMPPAPG.
    OrthoDBi EOG7KWSKG.
    PhylomeDBi Q75Q41.
    TreeFami TF106201.

    Enzyme and pathway databases

    Reactomei REACT_199211. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 646277.
    PROi Q75Q41.

    Gene expression databases

    Genevestigatori Q75Q41.

    Family and domain databases

    InterProi IPR005683. Tom22.
    [Graphical view ]
    Pfami PF04281. Tom22. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Targeting and assembly of rat mitochondrial translocase of outer membrane 22 (TOM22) into the TOM complex."
      Nakamura Y., Suzuki H., Sakaguchi M., Mihara K.
      J. Biol. Chem. 279:21223-21232(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    4. "Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A."
      Dagda R.K., Barwacz C.A., Cribbs J.T., Strack S.
      J. Biol. Chem. 280:27375-27382(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TOM COMPLEX, INTERACTION WITH PPP2R2B, MUTAGENESIS OF GLU-42; GLU-46; TRP-49 AND PRO-98.

    Entry informationi

    Entry nameiTOM22_RAT
    AccessioniPrimary (citable) accession number: Q75Q41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3