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Protein

Mitochondrial import receptor subunit TOM22 homolog

Gene

Tomm22

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore.

GO - Molecular functioni

  • protein transmembrane transporter activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM22 homolog
Short name:
rTOM22
Alternative name(s):
Translocase of outer membrane 22 kDa subunit homolog
Gene namesi
Name:Tomm22
Synonyms:Tom22
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1303260. Tomm22.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 82CytoplasmicSequence analysisAdd BLAST81
Transmembranei83 – 103HelicalSequence analysisAdd BLAST21
Topological domaini104 – 142Mitochondrial intermembraneSequence analysisAdd BLAST39

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42E → Q: Inhibits localization to the mitochondrion outer membrane. 1 Publication1
Mutagenesisi46E → P: Inhibits localization to the mitochondrion outer membrane. 1 Publication1
Mutagenesisi49W → P: Inhibits localization to the mitochondrion outer membrane. 1 Publication1
Mutagenesisi98P → A: Inhibits localization to the mitochondrion outer membrane. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003833592 – 142Mitochondrial import receptor subunit TOM22 homologAdd BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei43PhosphothreonineBy similarity1
Modified residuei45PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ75Q41.
PRIDEiQ75Q41.

PTM databases

iPTMnetiQ75Q41.
PhosphoSitePlusiQ75Q41.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014058.
GenevisibleiQ75Q41. RN.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOMM40 (By similarity). Interacts with PPP2R2B.By similarity1 Publication

Protein-protein interaction databases

IntActiQ75Q41. 1 interactor.
STRINGi10116.ENSRNOP00000019323.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 50Import sequence; necessary for mitochondrion outer membrane localization and integration in the TOM complex10
Regioni83 – 103TMD; necessary for mitochondrion outer membrane localization and integration in the TOM complexAdd BLAST21
Regioni123 – 142C-tail signal; necessary for mitochondrion outer membrane localization and integration in the TOM complexAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi110 – 118Poly-Gln9

Domaini

The N-terminal domain (residues 1-60) is important for binding to the unfolded mature imported proteins. Residues (47-69) of the cytoplasmic domain interacts with TOMM20 while the C-terminal segment (residues 61-80) binds presequence of preproteins (By similarity). Requires the transmembrane domain (TMD), a short segment (the import sequence) in the cytoplasmic domain localizing separately from the TMD and the C-tail signal in the C-terminal domain for efficient targeting and integration into the TOM complex.By similarity

Sequence similaritiesi

Belongs to the Tom22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4111. Eukaryota.
ENOG41122K3. LUCA.
GeneTreeiENSGT00390000016475.
HOGENOMiHOG000067816.
InParanoidiQ75Q41.
KOiK17769.
OMAiFYTFSCN.
OrthoDBiEOG091G0XN1.
PhylomeDBiQ75Q41.
TreeFamiTF106201.

Family and domain databases

InterProiIPR005683. Tom22.
[Graphical view]
PfamiPF04281. Tom22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75Q41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVAAAGA GEPLSPEELV PKAEAEKAEE DLEEDDDDEL DETLSERLWG
60 70 80 90 100
LTEMFPERVR SAAGATFDLS LFVAQKMYRF SRAALWIGTT SFMILVLPVV
110 120 130 140
FETEKLQMEQ QQQLQQRQIL LGPNTGLSGG MPGALPPLPG KI
Length:142
Mass (Da):15,491
Last modified:July 5, 2004 - v1
Checksum:iC1259302C15CA3AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162854 mRNA. Translation: BAD11364.1.
CH473950 Genomic DNA. Translation: EDM15790.1.
BC098639 mRNA. Translation: AAH98639.1.
RefSeqiNP_997679.1. NM_212514.2.
UniGeneiRn.3119.

Genome annotation databases

EnsembliENSRNOT00000019323; ENSRNOP00000019323; ENSRNOG00000014058.
GeneIDi300075.
KEGGirno:300075.
UCSCiRGD:1303260. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162854 mRNA. Translation: BAD11364.1.
CH473950 Genomic DNA. Translation: EDM15790.1.
BC098639 mRNA. Translation: AAH98639.1.
RefSeqiNP_997679.1. NM_212514.2.
UniGeneiRn.3119.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ75Q41. 1 interactor.
STRINGi10116.ENSRNOP00000019323.

PTM databases

iPTMnetiQ75Q41.
PhosphoSitePlusiQ75Q41.

Proteomic databases

PaxDbiQ75Q41.
PRIDEiQ75Q41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019323; ENSRNOP00000019323; ENSRNOG00000014058.
GeneIDi300075.
KEGGirno:300075.
UCSCiRGD:1303260. rat.

Organism-specific databases

CTDi56993.
RGDi1303260. Tomm22.

Phylogenomic databases

eggNOGiKOG4111. Eukaryota.
ENOG41122K3. LUCA.
GeneTreeiENSGT00390000016475.
HOGENOMiHOG000067816.
InParanoidiQ75Q41.
KOiK17769.
OMAiFYTFSCN.
OrthoDBiEOG091G0XN1.
PhylomeDBiQ75Q41.
TreeFamiTF106201.

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Miscellaneous databases

PROiQ75Q41.

Gene expression databases

BgeeiENSRNOG00000014058.
GenevisibleiQ75Q41. RN.

Family and domain databases

InterProiIPR005683. Tom22.
[Graphical view]
PfamiPF04281. Tom22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOM22_RAT
AccessioniPrimary (citable) accession number: Q75Q41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.