Q75Q41 (TOM22_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import receptor subunit TOM22 homolog
Short name=rTOM22

Alternative name(s):
Translocase of outer membrane 22 kDa subunit homolog

Gene names

Name:	Tomm22
Synonyms:	Tom22

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore.
Subunit structure	Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOMM40 By similarity. Interacts with PPP2R2B. Ref.4
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein.
Domain	The N-terminal domain (residues 1-60) is important for binding to the unfolded mature imported proteins. Residues (47-69) of the cytoplasmic domain interacts with TOMM20 while the C-terminal segment (residues 61-80) binds presequence of preproteins By similarity. Requires the transmembrane domain (TMD), a short segment (the import sequence) in the cytoplasmic domain localizing separately from the TMD and the C-tail signal in the C-terminal domain for efficient targeting and integration into the TOM complex.
Sequence similarities	Belongs to the Tom22 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial outer membrane Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: Compara mitochondrial outer membrane translocase complex Inferred from electronic annotation. Source: Compara
Molecular_function	protein transmembrane transporter activity Inferred from mutant phenotype PubMed 18687331. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 142	141	Mitochondrial import receptor subunit TOM22 homolog		PRO_0000383359
Regions
	Topological domain	2 – 82	81	Cytoplasmic Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Topological domain	104 – 142	39	Mitochondrial intermembrane Potential
	Region	41 – 50	10	Import sequence; necessary for mitochondrion outer membrane localization and integration in the TOM complex
	Region	83 – 103	21	TMD; necessary for mitochondrion outer membrane localization and integration in the TOM complex
	Region	123 – 142	20	C-tail signal; necessary for mitochondrion outer membrane localization and integration in the TOM complex
	Compositional bias	110 – 118	9	Poly-Gln
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	15	1	Phosphoserine By similarity
	Modified residue	43	1	Phosphothreonine By similarity
Experimental info
	Mutagenesis	42	1	E → Q: Inhibits localization to the mitochondrion outer membrane. Ref.4
	Mutagenesis	46	1	E → P: Inhibits localization to the mitochondrion outer membrane. Ref.4
	Mutagenesis	49	1	W → P: Inhibits localization to the mitochondrion outer membrane. Ref.4
	Mutagenesis	98	1	P → A: Inhibits localization to the mitochondrion outer membrane. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q75Q41 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C1259302C15CA3AB	FASTA	142	15,491
	10 20 30 40 50 60 MAAAVAAAGA GEPLSPEELV PKAEAEKAEE DLEEDDDDEL DETLSERLWG LTEMFPERVR 70 80 90 100 110 120 SAAGATFDLS LFVAQKMYRF SRAALWIGTT SFMILVLPVV FETEKLQMEQ QQQLQQRQIL 130 140 LGPNTGLSGG MPGALPPLPG KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Targeting and assembly of rat mitochondrial translocase of outer membrane 22 (TOM22) into the TOM complex." Nakamura Y., Suzuki H., Sakaguchi M., Mihara K. J. Biol. Chem. 279:21223-21232(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[4]	"Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A." Dagda R.K., Barwacz C.A., Cribbs J.T., Strack S. J. Biol. Chem. 280:27375-27382(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TOM COMPLEX, INTERACTION WITH PPP2R2B, MUTAGENESIS OF GLU-42; GLU-46; TRP-49 AND PRO-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB162854 mRNA. Translation: BAD11364.1. CH473950 Genomic DNA. Translation: EDM15790.1. BC098639 mRNA. Translation: AAH98639.1.
IPI	IPI00417749.
RefSeq	NP_997679.1. NM_212514.2.
UniGene	Rn.3119.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000019323.
Proteomic databases
PaxDb	Q75Q41.
PRIDE	Q75Q41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019323; ENSRNOP00000019323; ENSRNOG00000014058.
GeneID	300075.
KEGG	rno:300075.
UCSC	RGD:1303260. rat.
Organism-specific databases
CTD	56993.
RGD	1303260. Tomm22.
Phylogenomic databases
eggNOG	NOG310729.
GeneTree	ENSGT00390000016475.
HOGENOM	HOG000067816.
InParanoid	Q75Q41.
OMA	MMPPAPG.
OrthoDB	EOG441QD4.
Gene expression databases
Genevestigator	Q75Q41.
Family and domain databases
InterPro	IPR005683. Tom22. [Graphical view]
Pfam	PF04281. Tom22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	646277.

Entry information

Entry name

TOM22_RAT

Accession

Primary (citable) accession number: Q75Q41

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program