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Q75Q41 (TOM22_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import receptor subunit TOM22 homolog

Short name=rTOM22
Alternative name(s):
Translocase of outer membrane 22 kDa subunit homolog
Gene names
Name:Tomm22
Synonyms:Tom22
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length142 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore.

Subunit structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOMM40 By similarity. Interacts with PPP2R2B. Ref.4

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein.

Domain

The N-terminal domain (residues 1-60) is important for binding to the unfolded mature imported proteins. Residues (47-69) of the cytoplasmic domain interacts with TOMM20 while the C-terminal segment (residues 61-80) binds presequence of preproteins By similarity. Requires the transmembrane domain (TMD), a short segment (the import sequence) in the cytoplasmic domain localizing separately from the TMD and the C-tail signal in the C-terminal domain for efficient targeting and integration into the TOM complex.

Sequence similarities

Belongs to the Tom22 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 142141Mitochondrial import receptor subunit TOM22 homolog
PRO_0000383359

Regions

Topological domain2 – 8281Cytoplasmic Potential
Transmembrane83 – 10321Helical; Potential
Topological domain104 – 14239Mitochondrial intermembrane Potential
Region41 – 5010Import sequence; necessary for mitochondrion outer membrane localization and integration in the TOM complex
Region83 – 10321TMD; necessary for mitochondrion outer membrane localization and integration in the TOM complex
Region123 – 14220C-tail signal; necessary for mitochondrion outer membrane localization and integration in the TOM complex
Compositional bias110 – 1189Poly-Gln

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue151Phosphoserine By similarity
Modified residue431Phosphothreonine By similarity

Experimental info

Mutagenesis421E → Q: Inhibits localization to the mitochondrion outer membrane. Ref.4
Mutagenesis461E → P: Inhibits localization to the mitochondrion outer membrane. Ref.4
Mutagenesis491W → P: Inhibits localization to the mitochondrion outer membrane. Ref.4
Mutagenesis981P → A: Inhibits localization to the mitochondrion outer membrane. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q75Q41 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C1259302C15CA3AB

FASTA14215,491
        10         20         30         40         50         60 
MAAAVAAAGA GEPLSPEELV PKAEAEKAEE DLEEDDDDEL DETLSERLWG LTEMFPERVR 

        70         80         90        100        110        120 
SAAGATFDLS LFVAQKMYRF SRAALWIGTT SFMILVLPVV FETEKLQMEQ QQQLQQRQIL 

       130        140 
LGPNTGLSGG MPGALPPLPG KI 

« Hide

References

« Hide 'large scale' references
[1]"Targeting and assembly of rat mitochondrial translocase of outer membrane 22 (TOM22) into the TOM complex."
Nakamura Y., Suzuki H., Sakaguchi M., Mihara K.
J. Biol. Chem. 279:21223-21232(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A."
Dagda R.K., Barwacz C.A., Cribbs J.T., Strack S.
J. Biol. Chem. 280:27375-27382(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TOM COMPLEX, INTERACTION WITH PPP2R2B, MUTAGENESIS OF GLU-42; GLU-46; TRP-49 AND PRO-98.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB162854 mRNA. Translation: BAD11364.1.
CH473950 Genomic DNA. Translation: EDM15790.1.
BC098639 mRNA. Translation: AAH98639.1.
RefSeqNP_997679.1. NM_212514.2.
UniGeneRn.3119.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000019323.

Proteomic databases

PaxDbQ75Q41.
PRIDEQ75Q41.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019323; ENSRNOP00000019323; ENSRNOG00000014058.
GeneID300075.
KEGGrno:300075.
UCSCRGD:1303260. rat.

Organism-specific databases

CTD56993.
RGD1303260. Tomm22.

Phylogenomic databases

eggNOGNOG310729.
GeneTreeENSGT00390000016475.
HOGENOMHOG000067816.
InParanoidQ75Q41.
KOK17769.
OMAMMPPAPG.
OrthoDBEOG7KWSKG.
PhylomeDBQ75Q41.
TreeFamTF106201.

Gene expression databases

GenevestigatorQ75Q41.

Family and domain databases

InterProIPR005683. Tom22.
[Graphical view]
PfamPF04281. Tom22. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio646277.
PROQ75Q41.

Entry information

Entry nameTOM22_RAT
AccessionPrimary (citable) accession number: Q75Q41
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families