Reviewed, UniProtKB/Swiss-Prot Q75LR2 (AROF_ORYSJ)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase 1
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1
    DAHP synthetase 1

Gene names

Name:	DAHPS1
Ordered Locus Names:	Os03g0389700, LOC_Os03g27230
ORF Names:	OSJNBa0017N12.13

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the class-II DAHP synthetase family.
Sequence caution	The sequence AAR06362.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD14924.1 differs from that shown. Reason: Frameshift at positions 1 and 534. The sequence BAD14927.1 differs from that shown. Reason: Frameshift at positions 1 and 534.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Chloroplast Potential
	Chain	40 – 554	515	Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic		PRO_0000247310

Sequences

	Sequence		Length	Mass (Da)
	Q75LR2-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: CC3DB62F55DC79AC	FASTA	554	60,490
	10 20 30 40 50 60 MSLATSSSMA GGAAVVPRSA TATTASAFVT MKRRATAVRA VHAAEPSKNP PVGVPSAAKT 70 80 90 100 110 120 SSPSVAAPEK APVAAAPAPV APAPAATKQV APARWAVDSW RTKKALQLPE YPNAAELEAV 130 140 150 160 170 180 LKTIEAFPPI VFAGEARHLE ERLADAAMGR AFLLQGGDCA ESFKEFNGNN IRDTFRVLLQ 190 200 210 220 230 240 MSAVLTFGGQ MPVIKVGRMA GQFAKPRSEA FEERDGVKLP SYRGDNINGD AFNEKSRIPD 250 260 270 280 290 300 PQRMVRAYAQ SAATLNLLRA FATGGYAAMQ RVTQWNLDFT QHSEQGDRYR ELAHRVDEAL 310 320 330 340 350 360 GFMSAAGLTV DHPLMTSTDF WTSHECLLLP YEQSLTRQDS TTGHFYDCSA HMLWVGERTR 370 380 390 400 410 420 QLDGAHVEFL RGVANPLGIK VSDKMNPTEL VKLIEILNPS NKPGRITIIT RMGAENMRVK 430 440 450 460 470 480 LPHLIRAVRH AGQIVTWITD PMHGNTIKAP CGLKTRPFDS IAEVRAFFDV HDQEGSHPGG 490 500 510 520 530 540 VHLEMTGQNV TECIGGSRTV TFDDLGDRYH THCDPRLNAS QSLELSFIIA ERLRRKRIRS 550 SKLNNMLPLP PFGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cDNA encoding 3-deoxy-D-arabino heptulosonate-7-phosphate synthase expressed in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fujiminori.
[2]	"The gene encoding 3-deoxy-D-arabino heptulosonate-7-phosphate synthase expressed in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Fujiminori.
[3]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB122057 mRNA. Translation: BAD14924.1. Frameshift. AB122083 Genomic DNA. Translation: BAD14927.1. Frameshift. AC092075 Genomic DNA. Translation: AAR06362.1. Sequence problems.
RefSeq	NP_001050271.1.
UniGene	Os.7075
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4333014.
KEGG	osa:4333014.
Organism-specific databases
Gramene	Q75LR2.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_ORYSJ

Accession

Primary (citable) accession number: Q75LR2
Secondary accession number(s): Q75W15

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	September 22, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)