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Q75JR3 (IDHC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic
EC=1.1.1.42
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase 1
IDH 1
IDP 1
NADP(+)-specific ICDH 1
Oxalosuccinate decarboxylase 1
Gene names
Name:idhC
ORF Names:DDB_G0272208
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Isocitrate dehydrogenase [NADP] cytoplasmic
PRO_0000328190

Regions

Nucleotide binding76 – 783NADP By similarity
Nucleotide binding309 – 3146NADP By similarity
Region95 – 1017Substrate binding By similarity

Sites

Metal binding2531Magnesium or manganese By similarity
Metal binding2761Magnesium or manganese By similarity
Binding site781Substrate By similarity
Binding site831NADP By similarity
Binding site1101Substrate By similarity
Binding site1331Substrate By similarity
Binding site2611NADP By similarity
Binding site3271NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1401Critical for catalysis By similarity
Site2131Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q75JR3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 129878D79347E4A7

FASTA41246,370
        10         20         30         40         50         60 
MVEKIIVSNP VANLLGDEQT RVIWDLIEKK LIFPFLDLKV ETYDLGIEYR DKTNDQVTID 

        70         80         90        100        110        120 
AANAIKRLKV GIKCATITPD EARVTEFGLK EMWKSPNGTI RNTLGGTLFR EPIVCKNVPR 

       130        140        150        160        170        180 
LVTCWNKSIV IGRHAFGDQY RATDFVVKGA GKLELTYTPA DGSAPQKFQV FDFPSDGGVA 

       190        200        210        220        230        240 
LGMYNTDASI KEFAYACFNF SLDKKWPLYL STKNTILKRY DGRFKDIFQE IYEREYKVKF 

       250        260        270        280        290        300 
DTAGIWYEHR LIDDMVAYAM KSEGGFVWAC KNYDGDVQSD AVAQGYGSLG LMTSVLLSAD 

       310        320        330        340        350        360 
SLVAEASHGT VTRHFREHQK GRETSTNSIA SIFAWTRGLE YRAKLDNNDK LLKFCHALEA 

       370        380        390        400        410 
SCIDAVESGF MTKDLAICVK GSVENVKRTD YLNTEEYINK VAELLVSKLT AL

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000008 Genomic DNA. Translation: EAL71256.1.
RefSeqXP_645283.1. XM_640191.1.

3D structure databases

HSSPHSSP built from PDB template 1T0L based on UniProtKB O75874.
ProteinModelPortalQ75JR3.
SMRQ75JR3. Positions 2-409.
ModBaseSearch...

Protein-protein interaction databases

STRING44689.DDB_0231401.

Proteomic databases

PRIDEQ75JR3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231401; DDB0231401; DDB_G0272208.
GeneID8618449.
KEGGddi:DDB_G0272208.

Organism-specific databases

dictyBaseDDB_G0272208. idhC.

Phylogenomic databases

eggNOGCOG0538.
KOK00031.
OMAALGMFNT.
ProtClustDBPTZ00435.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDHC_DICDI
AccessionPrimary (citable) accession number: Q75JR3
Secondary accession number(s): Q559Q1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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