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Q75JR3

- IDHC_DICDI

UniProt

Q75JR3 - IDHC_DICDI

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Protein
Isocitrate dehydrogenase [NADP] cytoplasmic
Gene
idhC, DDB_G0272208
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781Substrate By similarity
Binding sitei83 – 831NADP By similarity
Binding sitei110 – 1101Substrate By similarity
Binding sitei133 – 1331Substrate By similarity
Sitei140 – 1401Critical for catalysis By similarity
Sitei213 – 2131Critical for catalysis By similarity
Metal bindingi253 – 2531Magnesium or manganese By similarity
Binding sitei261 – 2611NADP By similarity
Metal bindingi276 – 2761Magnesium or manganese By similarity
Binding sitei327 – 3271NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 783NADP By similarity
Nucleotide bindingi309 – 3146NADP By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: UniProtKB
  2. glyoxylate cycle Source: UniProtKB-KW
  3. isocitrate metabolic process Source: UniProtKB
  4. response to reactive oxygen species Source: dictyBase
  5. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_216230. NADPH regeneration.
REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase 1
IDH 1
IDP 1
NADP(+)-specific ICDH 1
Oxalosuccinate decarboxylase 1
Gene namesi
Name:idhC
ORF Names:DDB_G0272208
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272208. idhC.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: dictyBase
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Isocitrate dehydrogenase [NADP] cytoplasmic
PRO_0000328190Add
BLAST

Proteomic databases

PRIDEiQ75JR3.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0231401.

Structurei

3D structure databases

ProteinModelPortaliQ75JR3.
SMRiQ75JR3. Positions 2-409.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 1017Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0538.
KOiK00031.
OMAiSCGGVAM.
PhylomeDBiQ75JR3.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75JR3-1 [UniParc]FASTAAdd to Basket

« Hide

MVEKIIVSNP VANLLGDEQT RVIWDLIEKK LIFPFLDLKV ETYDLGIEYR    50
DKTNDQVTID AANAIKRLKV GIKCATITPD EARVTEFGLK EMWKSPNGTI 100
RNTLGGTLFR EPIVCKNVPR LVTCWNKSIV IGRHAFGDQY RATDFVVKGA 150
GKLELTYTPA DGSAPQKFQV FDFPSDGGVA LGMYNTDASI KEFAYACFNF 200
SLDKKWPLYL STKNTILKRY DGRFKDIFQE IYEREYKVKF DTAGIWYEHR 250
LIDDMVAYAM KSEGGFVWAC KNYDGDVQSD AVAQGYGSLG LMTSVLLSAD 300
SLVAEASHGT VTRHFREHQK GRETSTNSIA SIFAWTRGLE YRAKLDNNDK 350
LLKFCHALEA SCIDAVESGF MTKDLAICVK GSVENVKRTD YLNTEEYINK 400
VAELLVSKLT AL 412
Length:412
Mass (Da):46,370
Last modified:July 5, 2004 - v1
Checksum:i129878D79347E4A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71256.1.
RefSeqiXP_645283.1. XM_640191.1.

Genome annotation databases

EnsemblProtistsiDDB0231401; DDB0231401; DDB_G0272208.
GeneIDi8618449.
KEGGiddi:DDB_G0272208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71256.1 .
RefSeqi XP_645283.1. XM_640191.1.

3D structure databases

ProteinModelPortali Q75JR3.
SMRi Q75JR3. Positions 2-409.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231401.

Proteomic databases

PRIDEi Q75JR3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231401 ; DDB0231401 ; DDB_G0272208 .
GeneIDi 8618449.
KEGGi ddi:DDB_G0272208.

Organism-specific databases

dictyBasei DDB_G0272208. idhC.

Phylogenomic databases

eggNOGi COG0538.
KOi K00031.
OMAi SCGGVAM.
PhylomeDBi Q75JR3.

Enzyme and pathway databases

Reactomei REACT_216230. NADPH regeneration.
REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Miscellaneous databases

PROi Q75JR3.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiIDHC_DICDI
AccessioniPrimary (citable) accession number: Q75JR3
Secondary accession number(s): Q559Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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