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Q75JR3

- IDHC_DICDI

UniProt

Q75JR3 - IDHC_DICDI

Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

idhC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781SubstrateBy similarity
    Binding sitei83 – 831NADPBy similarity
    Binding sitei110 – 1101SubstrateBy similarity
    Binding sitei133 – 1331SubstrateBy similarity
    Sitei140 – 1401Critical for catalysisBy similarity
    Sitei213 – 2131Critical for catalysisBy similarity
    Metal bindingi253 – 2531Magnesium or manganeseBy similarity
    Binding sitei261 – 2611NADPBy similarity
    Metal bindingi276 – 2761Magnesium or manganeseBy similarity
    Binding sitei327 – 3271NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 783NADPBy similarity
    Nucleotide bindingi309 – 3146NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: UniProtKB
    2. glyoxylate cycle Source: UniProtKB-KW
    3. isocitrate metabolic process Source: UniProtKB
    4. response to reactive oxygen species Source: dictyBase
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_216230. NADPH regeneration.
    REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
    Alternative name(s):
    Cytosolic NADP-isocitrate dehydrogenase 1
    IDH 1
    IDP 1
    NADP(+)-specific ICDH 1
    Oxalosuccinate decarboxylase 1
    Gene namesi
    Name:idhC
    ORF Names:DDB_G0272208
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272208. idhC.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: dictyBase
    2. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412Isocitrate dehydrogenase [NADP] cytoplasmicPRO_0000328190Add
    BLAST

    Proteomic databases

    PRIDEiQ75JR3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231401.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75JR3.
    SMRiQ75JR3. Positions 2-409.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni95 – 1017Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0538.
    KOiK00031.
    OMAiSCGGVAM.
    PhylomeDBiQ75JR3.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q75JR3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEKIIVSNP VANLLGDEQT RVIWDLIEKK LIFPFLDLKV ETYDLGIEYR    50
    DKTNDQVTID AANAIKRLKV GIKCATITPD EARVTEFGLK EMWKSPNGTI 100
    RNTLGGTLFR EPIVCKNVPR LVTCWNKSIV IGRHAFGDQY RATDFVVKGA 150
    GKLELTYTPA DGSAPQKFQV FDFPSDGGVA LGMYNTDASI KEFAYACFNF 200
    SLDKKWPLYL STKNTILKRY DGRFKDIFQE IYEREYKVKF DTAGIWYEHR 250
    LIDDMVAYAM KSEGGFVWAC KNYDGDVQSD AVAQGYGSLG LMTSVLLSAD 300
    SLVAEASHGT VTRHFREHQK GRETSTNSIA SIFAWTRGLE YRAKLDNNDK 350
    LLKFCHALEA SCIDAVESGF MTKDLAICVK GSVENVKRTD YLNTEEYINK 400
    VAELLVSKLT AL 412
    Length:412
    Mass (Da):46,370
    Last modified:July 5, 2004 - v1
    Checksum:i129878D79347E4A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000008 Genomic DNA. Translation: EAL71256.1.
    RefSeqiXP_645283.1. XM_640191.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231401; DDB0231401; DDB_G0272208.
    GeneIDi8618449.
    KEGGiddi:DDB_G0272208.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000008 Genomic DNA. Translation: EAL71256.1 .
    RefSeqi XP_645283.1. XM_640191.1.

    3D structure databases

    ProteinModelPortali Q75JR3.
    SMRi Q75JR3. Positions 2-409.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231401.

    Proteomic databases

    PRIDEi Q75JR3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231401 ; DDB0231401 ; DDB_G0272208 .
    GeneIDi 8618449.
    KEGGi ddi:DDB_G0272208.

    Organism-specific databases

    dictyBasei DDB_G0272208. idhC.

    Phylogenomic databases

    eggNOGi COG0538.
    KOi K00031.
    OMAi SCGGVAM.
    PhylomeDBi Q75JR3.

    Enzyme and pathway databases

    Reactomei REACT_216230. NADPH regeneration.
    REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Miscellaneous databases

    PROi Q75JR3.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiIDHC_DICDI
    AccessioniPrimary (citable) accession number: Q75JR3
    Secondary accession number(s): Q559Q1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3