Reviewed, UniProtKB/Swiss-Prot Q75JR3 (IDHC_DICDI)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] cytoplasmic
    EC=1.1.1.42
Alternative name(s):
    IDH 1
    Cytosolic NADP-isocitrate dehydrogenase 1
    Oxalosuccinate decarboxylase 1
    NADP(+)-specific ICDH 1
    IDP 1

Gene names

Name:	idhC
ORF Names:	DDB_G0272208

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Isocitrate dehydrogenase [NADP] cytoplasmic		PRO_0000328190
Regions
	Nucleotide binding	76 – 78	3	NADP By similarity
	Nucleotide binding	309 – 314	6	NADP By similarity
	Region	95 – 101	7	Substrate binding By similarity
Sites
	Metal binding	253	1	Magnesium or manganese By similarity
	Metal binding	276	1	Magnesium or manganese By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	83	1	NADP By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	261	1	NADP By similarity
	Binding site	327	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	140	1	Critical for catalysis By similarity
	Site	213	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75JR3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 129878D79347E4A7	FASTA	412	46,370
	10 20 30 40 50 60 MVEKIIVSNP VANLLGDEQT RVIWDLIEKK LIFPFLDLKV ETYDLGIEYR DKTNDQVTID 70 80 90 100 110 120 AANAIKRLKV GIKCATITPD EARVTEFGLK EMWKSPNGTI RNTLGGTLFR EPIVCKNVPR 130 140 150 160 170 180 LVTCWNKSIV IGRHAFGDQY RATDFVVKGA GKLELTYTPA DGSAPQKFQV FDFPSDGGVA 190 200 210 220 230 240 LGMYNTDASI KEFAYACFNF SLDKKWPLYL STKNTILKRY DGRFKDIFQE IYEREYKVKF 250 260 270 280 290 300 DTAGIWYEHR LIDDMVAYAM KSEGGFVWAC KNYDGDVQSD AVAQGYGSLG LMTSVLLSAD 310 320 330 340 350 360 SLVAEASHGT VTRHFREHQK GRETSTNSIA SIFAWTRGLE YRAKLDNNDK LLKFCHALEA 370 380 390 400 410 SCIDAVESGF MTKDLAICVK GSVENVKRTD YLNTEEYINK VAELLVSKLT AL

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116963 Genomic DNA. Translation: AAS45361.1. AAFI02000008 Genomic DNA. Translation: EAL71256.1.
RefSeq	XP_645283.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3396432.
KEGG	ddi:DDB_0231401.
Organism-specific databases
dictyBase	DDB_G0272208. idhC.
Phylogenomic databases
OMA	Q75JR3. NRDATHD.
Enzyme and pathway databases
BRENDA	1.1.1.42. 424.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHC_DICDI

Accession

Primary (citable) accession number: Q75JR3
Secondary accession number(s): Q559Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)