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Q75JR2

- IDHP_DICDI

UniProt

Q75JR2 - IDHP_DICDI

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Protein

Isocitrate dehydrogenase [NADP], mitochondrial

Gene

idhM

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei105 – 1051NADPBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei155 – 1551SubstrateBy similarity
Sitei162 – 1621Critical for catalysisBy similarity
Sitei233 – 2331Critical for catalysisBy similarity
Metal bindingi273 – 2731Magnesium or manganeseBy similarity
Binding sitei281 – 2811NADPBy similarity
Metal bindingi296 – 2961Magnesium or manganeseBy similarity
Binding sitei348 – 3481NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1003NADPBy similarity
Nucleotide bindingi330 – 3356NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. NAD binding Source: InterPro

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: UniProtKB
  2. glyoxylate cycle Source: UniProtKB-KW
  3. isocitrate metabolic process Source: UniProtKB
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_216230. NADPH regeneration.
REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42)
Alternative name(s):
IDH 2
IDP 2
NADP(+)-specific ICDH 2
NADP-isocitrate dehydrogenase 2
Oxalosuccinate decarboxylase 2
Gene namesi
Name:idhM
ORF Names:DDB_G0272210
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272210. idhM.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
BLAST
Chaini28 – 428401Isocitrate dehydrogenase [NADP], mitochondrialPRO_0000328191Add
BLAST

Proteomic databases

PRIDEiQ75JR2.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0231402.

Structurei

3D structure databases

ProteinModelPortaliQ75JR2.
SMRiQ75JR2. Positions 23-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1237Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0538.
InParanoidiQ75JR2.
KOiK00031.
OMAiKVSREFY.
PhylomeDBiQ75JR2.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75JR2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISNISKKIL SNSSKFIQQQ SYSTKRIKVT GPVVEMDGDE QTRIMWESIK
60 70 80 90 100
SKLIFPYVDI TPEYYDLGLP NRDATNDQVT IDAANAIKRA KIGVKCATIT
110 120 130 140 150
PDEARVKEFG LKEMWKSPNG TIRNILDGTV FRGPIICKNL PLLVPGWKKP
160 170 180 190 200
IIIGRHAHGD QYKATDFVVN GPGKLEMIFT PSQGEPIKKV IYDFKGSGVA
210 220 230 240 250
MGMYNTTSSI TAFAHSCFQY AIDKKYPLYL STKNTILKKY DGQFKDIFQE
260 270 280 290 300
IYEREYSTKF GELGIWYEHR LIDDMVAFAL KSEGGFVWAC KNYDGDVQSD
310 320 330 340 350
IVAQGYGSLG LMTSVLTNAD GVFASEASHG TVTRHFREHQ KGNETSTNSV
360 370 380 390 400
ASIFAWTSAL GQRGKLDNNK DLVKFAQDME KACVESIESG FMTKDLAICI
410 420
KGNQVKRSNY LNTEEYINKV AEFYNKIK
Length:428
Mass (Da):48,064
Last modified:July 5, 2004 - v1
Checksum:iC91B168940882AE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71257.1.
RefSeqiXP_645284.1. XM_640192.1.

Genome annotation databases

EnsemblProtistsiDDB0231402; DDB0231402; DDB_G0272210.
GeneIDi8618450.
KEGGiddi:DDB_G0272210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71257.1 .
RefSeqi XP_645284.1. XM_640192.1.

3D structure databases

ProteinModelPortali Q75JR2.
SMRi Q75JR2. Positions 23-420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231402.

Proteomic databases

PRIDEi Q75JR2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231402 ; DDB0231402 ; DDB_G0272210 .
GeneIDi 8618450.
KEGGi ddi:DDB_G0272210.

Organism-specific databases

dictyBasei DDB_G0272210. idhM.

Phylogenomic databases

eggNOGi COG0538.
InParanoidi Q75JR2.
KOi K00031.
OMAi KVSREFY.
PhylomeDBi Q75JR2.

Enzyme and pathway databases

Reactomei REACT_216230. NADPH regeneration.
REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Miscellaneous databases

PROi Q75JR2.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiIDHP_DICDI
AccessioniPrimary (citable) accession number: Q75JR2
Secondary accession number(s): Q559Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3