Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q75JR2

- IDHP_DICDI

UniProt

Q75JR2 - IDHP_DICDI

Protein

Isocitrate dehydrogenase [NADP], mitochondrial

Gene

idhM

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei105 – 1051NADPBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei155 – 1551SubstrateBy similarity
    Sitei162 – 1621Critical for catalysisBy similarity
    Sitei233 – 2331Critical for catalysisBy similarity
    Metal bindingi273 – 2731Magnesium or manganeseBy similarity
    Binding sitei281 – 2811NADPBy similarity
    Metal bindingi296 – 2961Magnesium or manganeseBy similarity
    Binding sitei348 – 3481NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 1003NADPBy similarity
    Nucleotide bindingi330 – 3356NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: UniProtKB
    2. glyoxylate cycle Source: UniProtKB-KW
    3. isocitrate metabolic process Source: UniProtKB
    4. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_216230. NADPH regeneration.
    REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42)
    Alternative name(s):
    IDH 2
    IDP 2
    NADP(+)-specific ICDH 2
    NADP-isocitrate dehydrogenase 2
    Oxalosuccinate decarboxylase 2
    Gene namesi
    Name:idhM
    ORF Names:DDB_G0272210
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272210. idhM.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
    BLAST
    Chaini28 – 428401Isocitrate dehydrogenase [NADP], mitochondrialPRO_0000328191Add
    BLAST

    Proteomic databases

    PRIDEiQ75JR2.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231402.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75JR2.
    SMRiQ75JR2. Positions 23-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1237Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0538.
    KOiK00031.
    OMAiKVSREFY.
    PhylomeDBiQ75JR2.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q75JR2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISNISKKIL SNSSKFIQQQ SYSTKRIKVT GPVVEMDGDE QTRIMWESIK    50
    SKLIFPYVDI TPEYYDLGLP NRDATNDQVT IDAANAIKRA KIGVKCATIT 100
    PDEARVKEFG LKEMWKSPNG TIRNILDGTV FRGPIICKNL PLLVPGWKKP 150
    IIIGRHAHGD QYKATDFVVN GPGKLEMIFT PSQGEPIKKV IYDFKGSGVA 200
    MGMYNTTSSI TAFAHSCFQY AIDKKYPLYL STKNTILKKY DGQFKDIFQE 250
    IYEREYSTKF GELGIWYEHR LIDDMVAFAL KSEGGFVWAC KNYDGDVQSD 300
    IVAQGYGSLG LMTSVLTNAD GVFASEASHG TVTRHFREHQ KGNETSTNSV 350
    ASIFAWTSAL GQRGKLDNNK DLVKFAQDME KACVESIESG FMTKDLAICI 400
    KGNQVKRSNY LNTEEYINKV AEFYNKIK 428
    Length:428
    Mass (Da):48,064
    Last modified:July 5, 2004 - v1
    Checksum:iC91B168940882AE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000008 Genomic DNA. Translation: EAL71257.1.
    RefSeqiXP_645284.1. XM_640192.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231402; DDB0231402; DDB_G0272210.
    GeneIDi8618450.
    KEGGiddi:DDB_G0272210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000008 Genomic DNA. Translation: EAL71257.1 .
    RefSeqi XP_645284.1. XM_640192.1.

    3D structure databases

    ProteinModelPortali Q75JR2.
    SMRi Q75JR2. Positions 23-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231402.

    Proteomic databases

    PRIDEi Q75JR2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231402 ; DDB0231402 ; DDB_G0272210 .
    GeneIDi 8618450.
    KEGGi ddi:DDB_G0272210.

    Organism-specific databases

    dictyBasei DDB_G0272210. idhM.

    Phylogenomic databases

    eggNOGi COG0538.
    KOi K00031.
    OMAi KVSREFY.
    PhylomeDBi Q75JR2.

    Enzyme and pathway databases

    Reactomei REACT_216230. NADPH regeneration.
    REACT_221507. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Miscellaneous databases

    PROi Q75JR2.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiIDHP_DICDI
    AccessioniPrimary (citable) accession number: Q75JR2
    Secondary accession number(s): Q559Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3