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Q75JR2 (IDHP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP], mitochondrial
EC=1.1.1.42
Alternative name(s):
IDH 2
IDP 2
NADP(+)-specific ICDH 2
NADP-isocitrate dehydrogenase 2
Oxalosuccinate decarboxylase 2
Gene names
Name:idhM
ORF Names:DDB_G0272210
OrganismDictyostelium discoideum (Slime mold)
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion Potential.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 428401Isocitrate dehydrogenase [NADP], mitochondrial
PRO_0000328191

Regions

Nucleotide binding98 – 1003NADP By similarity
Nucleotide binding330 – 3356NADP By similarity
Region117 – 1237Substrate binding By similarity

Sites

Metal binding2731Magnesium or manganese By similarity
Metal binding2961Magnesium or manganese By similarity
Binding site1001Substrate By similarity
Binding site1051NADP By similarity
Binding site1321Substrate By similarity
Binding site1551Substrate By similarity
Binding site2811NADP By similarity
Binding site3481NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1621Critical for catalysis By similarity
Site2331Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q75JR2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C91B168940882AE1

FASTA42848,064
        10         20         30         40         50         60 
MISNISKKIL SNSSKFIQQQ SYSTKRIKVT GPVVEMDGDE QTRIMWESIK SKLIFPYVDI 

        70         80         90        100        110        120 
TPEYYDLGLP NRDATNDQVT IDAANAIKRA KIGVKCATIT PDEARVKEFG LKEMWKSPNG 

       130        140        150        160        170        180 
TIRNILDGTV FRGPIICKNL PLLVPGWKKP IIIGRHAHGD QYKATDFVVN GPGKLEMIFT 

       190        200        210        220        230        240 
PSQGEPIKKV IYDFKGSGVA MGMYNTTSSI TAFAHSCFQY AIDKKYPLYL STKNTILKKY 

       250        260        270        280        290        300 
DGQFKDIFQE IYEREYSTKF GELGIWYEHR LIDDMVAFAL KSEGGFVWAC KNYDGDVQSD 

       310        320        330        340        350        360 
IVAQGYGSLG LMTSVLTNAD GVFASEASHG TVTRHFREHQ KGNETSTNSV ASIFAWTSAL 

       370        380        390        400        410        420 
GQRGKLDNNK DLVKFAQDME KACVESIESG FMTKDLAICI KGNQVKRSNY LNTEEYINKV 


AEFYNKIK

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed: 12097910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000008 Genomic DNA. Translation: EAL71257.1.
RefSeqXP_645284.1. XM_640192.1.

3D structure databases

HSSPHSSP built from PDB template 1T0L based on UniProtKB O75874.
ProteinModelPortalQ75JR2.
SMRQ75JR2. Positions 23-420.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ75JR2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231402; DDB0231402; DDB_G0272210.
GeneID8618450.
GenomeReviewsGene locus idhM in contig CM000151_GR.
KEGGddi:DDB_G0272210.

Organism-specific databases

dictyBaseDDB_G0272210. idhM.

Phylogenomic databases

eggNOGKOG1526.
GeneTreeEPrGT00050000005787.
HOGENOMHBG596047.
OMAFKFPDAG.
PhylomeDBQ75JR2.
ProtClustDBPTZ00435.

Family and domain databases

InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP_euk-typ.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00031.
PANTHERPTHR11822. IDH_NADP_euk. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. Nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDHP_DICDI
AccessionPrimary (citable) accession number: Q75JR2
Secondary accession number(s): Q559Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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