Reviewed, UniProtKB/Swiss-Prot Q75JR2 (IDHP_DICDI)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
    EC=1.1.1.42
Alternative name(s):
    IDH 2
    NADP-isocitrate dehydrogenase 2
    Oxalosuccinate decarboxylase 2
    NADP(+)-specific ICDH 2
    IDP 2

Gene names

Name:	idhM
ORF Names:	DDB_G0272210

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	MitochondrionPotential.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion Potential
	Chain	28 – 428	401	Isocitrate dehydrogenase [NADP], mitochondrial		PRO_0000328191
Regions
	Nucleotide binding	98 – 100	3	NADP By similarity
	Nucleotide binding	330 – 335	6	NADP By similarity
	Region	117 – 123	7	Substrate binding By similarity
Sites
	Metal binding	273	1	Magnesium or manganese By similarity
	Metal binding	296	1	Magnesium or manganese By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	105	1	NADP By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	281	1	NADP By similarity
	Binding site	348	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	162	1	Critical for catalysis By similarity
	Site	233	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75JR2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C91B168940882AE1	FASTA	428	48,064
	10 20 30 40 50 60 MISNISKKIL SNSSKFIQQQ SYSTKRIKVT GPVVEMDGDE QTRIMWESIK SKLIFPYVDI 70 80 90 100 110 120 TPEYYDLGLP NRDATNDQVT IDAANAIKRA KIGVKCATIT PDEARVKEFG LKEMWKSPNG 130 140 150 160 170 180 TIRNILDGTV FRGPIICKNL PLLVPGWKKP IIIGRHAHGD QYKATDFVVN GPGKLEMIFT 190 200 210 220 230 240 PSQGEPIKKV IYDFKGSGVA MGMYNTTSSI TAFAHSCFQY AIDKKYPLYL STKNTILKKY 250 260 270 280 290 300 DGQFKDIFQE IYEREYSTKF GELGIWYEHR LIDDMVAFAL KSEGGFVWAC KNYDGDVQSD 310 320 330 340 350 360 IVAQGYGSLG LMTSVLTNAD GVFASEASHG TVTRHFREHQ KGNETSTNSV ASIFAWTSAL 370 380 390 400 410 420 GQRGKLDNNK DLVKFAQDME KACVESIESG FMTKDLAICI KGNQVKRSNY LNTEEYINKV AEFYNKIK

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116963 Genomic DNA. Translation: AAS45362.1. AAFI02000008 Genomic DNA. Translation: EAL71257.1.
RefSeq	XP_645284.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3396433.
KEGG	ddi:DDB_0231402.
Organism-specific databases
dictyBase	DDB_G0272210. idhM.
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHP_DICDI

Accession

Primary (citable) accession number: Q75JR2
Secondary accession number(s): Q559Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)