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Protein

Isocitrate dehydrogenase [NADP], mitochondrial

Gene

idhM

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Binding sitei105NADPBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1
Sitei162Critical for catalysisBy similarity1
Sitei233Critical for catalysisBy similarity1
Metal bindingi273Magnesium or manganeseBy similarity1
Binding sitei281NADPBy similarity1
Metal bindingi296Magnesium or manganeseBy similarity1
Binding sitei348NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 100NADPBy similarity3
Nucleotide bindingi330 – 335NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-DDI-389542. NADPH regeneration.
R-DDI-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42)
Alternative name(s):
IDH 2
IDP 2
NADP(+)-specific ICDH 2
NADP-isocitrate dehydrogenase 2
Oxalosuccinate decarboxylase 2
Gene namesi
Name:idhM
ORF Names:DDB_G0272210
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272210. idhM.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000032819128 – 428Isocitrate dehydrogenase [NADP], mitochondrialAdd BLAST401

Proteomic databases

PaxDbiQ75JR2.
PRIDEiQ75JR2.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231402.

Structurei

3D structure databases

ProteinModelPortaliQ75JR2.
SMRiQ75JR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 123Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
InParanoidiQ75JR2.
KOiK00031.
OMAiEGFAHAS.
PhylomeDBiQ75JR2.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75JR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISNISKKIL SNSSKFIQQQ SYSTKRIKVT GPVVEMDGDE QTRIMWESIK
60 70 80 90 100
SKLIFPYVDI TPEYYDLGLP NRDATNDQVT IDAANAIKRA KIGVKCATIT
110 120 130 140 150
PDEARVKEFG LKEMWKSPNG TIRNILDGTV FRGPIICKNL PLLVPGWKKP
160 170 180 190 200
IIIGRHAHGD QYKATDFVVN GPGKLEMIFT PSQGEPIKKV IYDFKGSGVA
210 220 230 240 250
MGMYNTTSSI TAFAHSCFQY AIDKKYPLYL STKNTILKKY DGQFKDIFQE
260 270 280 290 300
IYEREYSTKF GELGIWYEHR LIDDMVAFAL KSEGGFVWAC KNYDGDVQSD
310 320 330 340 350
IVAQGYGSLG LMTSVLTNAD GVFASEASHG TVTRHFREHQ KGNETSTNSV
360 370 380 390 400
ASIFAWTSAL GQRGKLDNNK DLVKFAQDME KACVESIESG FMTKDLAICI
410 420
KGNQVKRSNY LNTEEYINKV AEFYNKIK
Length:428
Mass (Da):48,064
Last modified:July 5, 2004 - v1
Checksum:iC91B168940882AE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71257.1.
RefSeqiXP_645284.1. XM_640192.1.

Genome annotation databases

EnsemblProtistsiEAL71257; EAL71257; DDB_G0272210.
GeneIDi8618450.
KEGGiddi:DDB_G0272210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71257.1.
RefSeqiXP_645284.1. XM_640192.1.

3D structure databases

ProteinModelPortaliQ75JR2.
SMRiQ75JR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231402.

Proteomic databases

PaxDbiQ75JR2.
PRIDEiQ75JR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL71257; EAL71257; DDB_G0272210.
GeneIDi8618450.
KEGGiddi:DDB_G0272210.

Organism-specific databases

dictyBaseiDDB_G0272210. idhM.

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
InParanoidiQ75JR2.
KOiK00031.
OMAiEGFAHAS.
PhylomeDBiQ75JR2.

Enzyme and pathway databases

ReactomeiR-DDI-389542. NADPH regeneration.
R-DDI-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ75JR2.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDHP_DICDI
AccessioniPrimary (citable) accession number: Q75JR2
Secondary accession number(s): Q559Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.