Reviewed, UniProtKB/Swiss-Prot Q75JQ1 (SYDC1_DICDI)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase, cytoplasmic 1
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase 1
      Short name=AspRS 1

Gene names

Name:	aspS1
ORF Names:	DDB_G0272236

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro response to bacterium Inferred from expression pattern. Source: dictyBase
Cellular component	phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Aspartyl-tRNA synthetase, cytoplasmic 1		PRO_0000327967

Sequences

	Sequence		Length	Mass (Da)
	Q75JQ1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E4AFCB20CB79F996	FASTA	576	64,935
	10 20 30 40 50 60 MSETTPVPVG EAGEKSATAL KKEQKKLEKE KKIAEAKAKK AAEKSAASGN SGEQKNEKKQ 70 80 90 100 110 120 EQVEENNSNW GELPMNQSKE KITREWTDVS QLTESLVGKS VLIRARLSTS RLQGANLCFV 130 140 150 160 170 180 QLRDGLYTVQ AVVAKGGSNS KSMVQFVGQV PKESIVDVQA TVVSTSVPIE SCTQKSVELQ 190 200 210 220 230 240 VSSFFIVSKS TLLPLQIEDL SRAQPLLDKQ EDDLKQLEQL LQNTNLNEQE KTDLEKKKSE 250 260 270 280 290 300 CIKFVNVSQE KRLDNRALDL RVPAHQSIFR LQSGVCTLFR EQLLGEGFIE IHSPKIISAA 310 320 330 340 350 360 SESGASVFKL NYFNTHAYLA QSPQLYKQMA ICADFNKVFE IGPVFRAENS NTHRHLTEFV 370 380 390 400 410 420 GLDLEMTFKD HYHEALDTLD RLMTSIFRGL ETRFAKEIES VNTQYPFEPF KFTYPSPRFT 430 440 450 460 470 480 FDEAAAMLAE LNDPDYIVKD NDFNTRQEKR LGKIIKEKFG VDFFIVDKFH VEVRPFYTMP 490 500 510 520 530 540 DPNNPQWANA YDLFMRGEEI CSGAQRIHDP ELLEKSAKSH GVVIEDIQGY IDSFKYGCSQ 550 560 570 HAGGGVGLER VVMLYLGLGN IRKASFCPRD PTRLTP

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL71270.1.
RefSeq	XP_645306.1.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
SMR	Q75JQ1. Positions 67-576.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75JQ1.
Genome annotation databases
GeneID	8618472.
KEGG	ddi:DDB_0231308.
Organism-specific databases
dictyBase	DDB_G0272236. aspS1.
Phylogenomic databases
eggNOG	KOG0556.
HOGENOM	HBG745843.
OMA	RNPKQSN.
PhylomeDB	Q75JQ1.
Enzyme and pathway databases
BRENDA	6.1.1.12. 424.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR004523. Asp-tRNA-synth_IIb_arc/euk. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00458. aspS_arch. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYDC1_DICDI

Accession

Primary (citable) accession number: Q75JQ1
Secondary accession number(s): Q559M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)