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Q75JI3

- NSF_DICDI

UniProt

Q75JI3 - NSF_DICDI

Protein

Vesicle-fusing ATPase

Gene

nsfA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Involved in endocytosis and endosome-endosome fusion. May be required for transport from the endoplasmic reticulum to the Golgi stack, and for the fusion of transport vesicles within the Golgi cisternae. Required for cell polarity, locomotion and chemotaxis.7 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi507 – 5126ATPBy similarity
    Nucleotide bindingi547 – 5548ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleoside-triphosphatase activity Source: InterPro
    4. protein binding Source: IntAct

    GO - Biological processi

    1. cellular component movement Source: dictyBase
    2. chemotaxis Source: UniProtKB-KW
    3. endocytosis Source: dictyBase
    4. phagocytosis Source: dictyBase
    5. protein secretion Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Chemotaxis, ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive factor A
    NEM-sensitive fusion protein A
    Vesicular-fusion protein nfsA
    Gene namesi
    Name:nsfA
    ORF Names:DDB_G0276153
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0276153. nsfA.

    Subcellular locationi

    Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. endosome membrane Source: UniProtKB-SubCell
    3. membrane Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 738738Vesicle-fusing ATPasePRO_0000327890Add
    BLAST

    Proteomic databases

    PRIDEiQ75JI3.

    Expressioni

    Developmental stagei

    Expressed in relatively constant amounts throughout the entire differentiation cycle.1 Publication

    Interactioni

    Subunit structurei

    Interacts with syn7A, snpA and snpC.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    snpAQ54NP62EBI-1810142,EBI-1810173
    snpCQ9U9R72EBI-1810142,EBI-1810184
    syn7AQ54JY72EBI-1810142,EBI-1810238

    Protein-protein interaction databases

    IntActiQ75JI3. 3 interactions.
    STRINGi44689.DDB_0185052.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75JI3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    KOiK06027.
    OMAiMTIELEF.
    PhylomeDBiQ75JI3.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q75JI3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYPPPVGDS IKLKVQASND PEEAFTNRAY LPISSFSFLF PNVQSNLYTT    50
    NTNYIKIRVG ANEYILSASP NKNMKPDSIA LSKALRGWMY VSNNEEVYVE 100
    FYDPNPNICG SMKVSIDYLT KGKQGPKQDS QEIIGKIIDN FNSQYFTFGQ 150
    LFYIKNSNST FELRVEAVET NEVPTKDKGW AIISPATKII LQKMPGSLID 200
    IETNGPLVVN QIFTSDWDFE NMGIGGLDAE FRDIFRRAFS SRIFPPAIVK 250
    KLGVNHVKGM LLYGPPGTGK TLIARQIGKM LNGREPKVVS GPSILNKYVG 300
    QSEENIRMLF RDAEIEQKAK GDDSGLHIII FDELDAICKS RGSRQGDSGV 350
    GDSVVNQLLA MIDGVESLNN ILVIGMTNRK DMIDEALLRP GRLEVHVEIS 400
    LPDEHGREQI FKIHTAKMRD QNALDKDVNL ANYAHTTRNY SGAEIEGVVK 450
    SAASYAFSRQ VDTKNIKNVE IKPEDIKVCD QDFKRAITEV TPSFGSTDNQ 500
    FESYAENGII NYGPVFDKLL QSGNAFVEQV KKSNRTPMMS VLLSGRPGCG 550
    KSSLAATLAK SSEFPFTRII SPNDLLGYNE SAKASKITKV FEDSYKSPMS 600
    CVVVDEIERL IEYVPIGPRF SNLILQTLAV LFKRTPPKGR KLLVIATTSN 650
    PDILKDMDIM DCFATVLSVP SISTAKEFQT VCFELGFTQK EASEAASFFT 700
    SPITIKQIIM IVEMARQEEG NFIDNFKMCL EDFNIRNF 738
    Length:738
    Mass (Da):82,233
    Last modified:July 5, 2004 - v1
    Checksum:iCD1AF65CF49BDB64
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti501 – 5011F → L in AAC48226. (PubMed:9511743)Curated
    Sequence conflicti592 – 5921E → D in AAC48226. (PubMed:9511743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006826 mRNA. Translation: AAC48226.1.
    AAFI02000014 Genomic DNA. Translation: EAL69376.1.
    RefSeqiXP_643273.1. XM_638181.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185052; DDB0185052; DDB_G0276153.
    GeneIDi8620316.
    KEGGiddi:DDB_G0276153.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006826 mRNA. Translation: AAC48226.1 .
    AAFI02000014 Genomic DNA. Translation: EAL69376.1 .
    RefSeqi XP_643273.1. XM_638181.1.

    3D structure databases

    ProteinModelPortali Q75JI3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q75JI3. 3 interactions.
    STRINGi 44689.DDB_0185052.

    Proteomic databases

    PRIDEi Q75JI3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185052 ; DDB0185052 ; DDB_G0276153 .
    GeneIDi 8620316.
    KEGGi ddi:DDB_G0276153.

    Organism-specific databases

    dictyBasei DDB_G0276153. nsfA.

    Phylogenomic databases

    eggNOGi COG0464.
    KOi K06027.
    OMAi MTIELEF.
    PhylomeDBi Q75JI3.

    Miscellaneous databases

    PROi Q75JI3.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein."
      Weidenhaupt M., Bruckert F., Satre M.
      Gene 207:53-60(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Strain: AX2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "Characterization of endosome-endosome fusion in a cell-free system using Dictyostelium discoideum."
      Lenhard J.M., Mayorga L., Stahl P.D.
      J. Biol. Chem. 267:1896-1903(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "In vitro reconstituted Dictyostelium discoideum early endosome fusion is regulated by Rab7 but proceeds in the absence of ATP-Mg2+ from the bulk solution."
      Laurent O., Bruckert F., Adessi C., Satre M.
      J. Biol. Chem. 273:793-799(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Functional and molecular identification of novel members of the ubiquitous membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-sensitive factor-attachment proteins) families in Dictyostelium discoideum."
      Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M.
      Eur. J. Biochem. 267:2062-2070(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SNPA AND SNPC.
    7. "A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion."
      Bogdanovic A., Bruckert F., Morio T., Satre M.
      J. Biol. Chem. 275:36691-36697(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SYN7A.
    8. "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum."
      Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J., Satre M., Bruckert F.
      Biochem. J. 368:29-39(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SYN7A, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Cell polarity and locomotion, as well as endocytosis, depend on NSF."
      Thompson C.R.L., Bretscher M.S.
      Development 129:4185-4192(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Possible roles of the endocytic cycle in cell motility."
      Traynor D., Kay R.R.
      J. Cell Sci. 120:2318-2327(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNSF_DICDI
    AccessioniPrimary (citable) accession number: Q75JI3
    Secondary accession number(s): O15646, Q552E0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3