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Q75JI3 (NSF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-fusing ATPase

EC=3.6.4.6
Alternative name(s):
N-ethylmaleimide-sensitive factor A
NEM-sensitive fusion protein A
Vesicular-fusion protein nfsA
Gene names
Name:nsfA
ORF Names:DDB_G0276153
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length738 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vesicle-mediated transport. Involved in endocytosis and endosome-endosome fusion. May be required for transport from the endoplasmic reticulum to the Golgi stack, and for the fusion of transport vesicles within the Golgi cisternae. Required for cell polarity, locomotion and chemotaxis. Ref.1 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Interacts with syn7A, snpA and snpC. Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Ref.6.

Developmental stage

Expressed in relatively constant amounts throughout the entire differentiation cycle. Ref.1

Sequence similarities

Belongs to the AAA ATPase family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 738738Vesicle-fusing ATPase
PRO_0000327890

Regions

Nucleotide binding507 – 5126ATP By similarity
Nucleotide binding547 – 5548ATP By similarity

Experimental info

Sequence conflict5011F → L in AAC48226. Ref.1
Sequence conflict5921E → D in AAC48226. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q75JI3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: CD1AF65CF49BDB64

FASTA73882,233
        10         20         30         40         50         60 
MSYPPPVGDS IKLKVQASND PEEAFTNRAY LPISSFSFLF PNVQSNLYTT NTNYIKIRVG 

        70         80         90        100        110        120 
ANEYILSASP NKNMKPDSIA LSKALRGWMY VSNNEEVYVE FYDPNPNICG SMKVSIDYLT 

       130        140        150        160        170        180 
KGKQGPKQDS QEIIGKIIDN FNSQYFTFGQ LFYIKNSNST FELRVEAVET NEVPTKDKGW 

       190        200        210        220        230        240 
AIISPATKII LQKMPGSLID IETNGPLVVN QIFTSDWDFE NMGIGGLDAE FRDIFRRAFS 

       250        260        270        280        290        300 
SRIFPPAIVK KLGVNHVKGM LLYGPPGTGK TLIARQIGKM LNGREPKVVS GPSILNKYVG 

       310        320        330        340        350        360 
QSEENIRMLF RDAEIEQKAK GDDSGLHIII FDELDAICKS RGSRQGDSGV GDSVVNQLLA 

       370        380        390        400        410        420 
MIDGVESLNN ILVIGMTNRK DMIDEALLRP GRLEVHVEIS LPDEHGREQI FKIHTAKMRD 

       430        440        450        460        470        480 
QNALDKDVNL ANYAHTTRNY SGAEIEGVVK SAASYAFSRQ VDTKNIKNVE IKPEDIKVCD 

       490        500        510        520        530        540 
QDFKRAITEV TPSFGSTDNQ FESYAENGII NYGPVFDKLL QSGNAFVEQV KKSNRTPMMS 

       550        560        570        580        590        600 
VLLSGRPGCG KSSLAATLAK SSEFPFTRII SPNDLLGYNE SAKASKITKV FEDSYKSPMS 

       610        620        630        640        650        660 
CVVVDEIERL IEYVPIGPRF SNLILQTLAV LFKRTPPKGR KLLVIATTSN PDILKDMDIM 

       670        680        690        700        710        720 
DCFATVLSVP SISTAKEFQT VCFELGFTQK EASEAASFFT SPITIKQIIM IVEMARQEEG 

       730 
NFIDNFKMCL EDFNIRNF 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein."
Weidenhaupt M., Bruckert F., Satre M.
Gene 207:53-60(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Strain: AX2.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"Characterization of endosome-endosome fusion in a cell-free system using Dictyostelium discoideum."
Lenhard J.M., Mayorga L., Stahl P.D.
J. Biol. Chem. 267:1896-1903(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"In vitro reconstituted Dictyostelium discoideum early endosome fusion is regulated by Rab7 but proceeds in the absence of ATP-Mg2+ from the bulk solution."
Laurent O., Bruckert F., Adessi C., Satre M.
J. Biol. Chem. 273:793-799(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Functional and molecular identification of novel members of the ubiquitous membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-sensitive factor-attachment proteins) families in Dictyostelium discoideum."
Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M.
Eur. J. Biochem. 267:2062-2070(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SNPA AND SNPC.
[7]"A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion."
Bogdanovic A., Bruckert F., Morio T., Satre M.
J. Biol. Chem. 275:36691-36697(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SYN7A.
[8]"Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum."
Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J., Satre M., Bruckert F.
Biochem. J. 368:29-39(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SYN7A, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Cell polarity and locomotion, as well as endocytosis, depend on NSF."
Thompson C.R.L., Bretscher M.S.
Development 129:4185-4192(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Possible roles of the endocytic cycle in cell motility."
Traynor D., Kay R.R.
J. Cell Sci. 120:2318-2327(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF006826 mRNA. Translation: AAC48226.1.
AAFI02000014 Genomic DNA. Translation: EAL69376.1.
RefSeqXP_643273.1. XM_638181.1.

3D structure databases

ProteinModelPortalQ75JI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ75JI3. 3 interactions.
STRING44689.DDB_0185052.

Proteomic databases

PRIDEQ75JI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185052; DDB0185052; DDB_G0276153.
GeneID8620316.
KEGGddi:DDB_G0276153.

Organism-specific databases

dictyBaseDDB_G0276153. nsfA.

Phylogenomic databases

eggNOGCOG0464.
OMAMTIELEF.
PhylomeDBQ75JI3.

Family and domain databases

Gene3D3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ75JI3.

Entry information

Entry nameNSF_DICDI
AccessionPrimary (citable) accession number: Q75JI3
Secondary accession number(s): O15646, Q552E0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase