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Protein

Vesicle-fusing ATPase

Gene

nsfA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Involved in endocytosis and endosome-endosome fusion. May be required for transport from the endoplasmic reticulum to the Golgi stack, and for the fusion of transport vesicles within the Golgi cisternae. Required for cell polarity, locomotion and chemotaxis.7 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi507 – 512ATPBy similarity6
Nucleotide bindingi547 – 554ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell motility Source: dictyBase
  • chemotaxis Source: UniProtKB-KW
  • endocytosis Source: dictyBase
  • Golgi to plasma membrane protein transport Source: GO_Central
  • Golgi vesicle docking Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • phagocytosis Source: dictyBase
  • protein secretion Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Chemotaxis, ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-DDI-6807878. COPI-mediated anterograde transport.
R-DDI-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive factor A
NEM-sensitive fusion protein A
Vesicular-fusion protein nfsA
Gene namesi
Name:nsfA
ORF Names:DDB_G0276153
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276153. nsfA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • endosome membrane Source: UniProtKB-SubCell
  • Golgi stack Source: GO_Central
  • membrane Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003278901 – 738Vesicle-fusing ATPaseAdd BLAST738

Proteomic databases

PaxDbiQ75JI3.

Expressioni

Developmental stagei

Expressed in relatively constant amounts throughout the entire differentiation cycle.1 Publication

Interactioni

Subunit structurei

Interacts with syn7A, snpA and snpC.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
snpAQ54NP62EBI-1810142,EBI-1810173
snpCQ9U9R72EBI-1810142,EBI-1810184
syn7AQ54JY72EBI-1810142,EBI-1810238

Protein-protein interaction databases

IntActiQ75JI3. 3 interactors.
STRINGi44689.DDB0185052.

Structurei

3D structure databases

ProteinModelPortaliQ75JI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741. Eukaryota.
COG0464. LUCA.
InParanoidiQ75JI3.
KOiK06027.
OMAiFDSADIN.
PhylomeDBiQ75JI3.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75JI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYPPPVGDS IKLKVQASND PEEAFTNRAY LPISSFSFLF PNVQSNLYTT
60 70 80 90 100
NTNYIKIRVG ANEYILSASP NKNMKPDSIA LSKALRGWMY VSNNEEVYVE
110 120 130 140 150
FYDPNPNICG SMKVSIDYLT KGKQGPKQDS QEIIGKIIDN FNSQYFTFGQ
160 170 180 190 200
LFYIKNSNST FELRVEAVET NEVPTKDKGW AIISPATKII LQKMPGSLID
210 220 230 240 250
IETNGPLVVN QIFTSDWDFE NMGIGGLDAE FRDIFRRAFS SRIFPPAIVK
260 270 280 290 300
KLGVNHVKGM LLYGPPGTGK TLIARQIGKM LNGREPKVVS GPSILNKYVG
310 320 330 340 350
QSEENIRMLF RDAEIEQKAK GDDSGLHIII FDELDAICKS RGSRQGDSGV
360 370 380 390 400
GDSVVNQLLA MIDGVESLNN ILVIGMTNRK DMIDEALLRP GRLEVHVEIS
410 420 430 440 450
LPDEHGREQI FKIHTAKMRD QNALDKDVNL ANYAHTTRNY SGAEIEGVVK
460 470 480 490 500
SAASYAFSRQ VDTKNIKNVE IKPEDIKVCD QDFKRAITEV TPSFGSTDNQ
510 520 530 540 550
FESYAENGII NYGPVFDKLL QSGNAFVEQV KKSNRTPMMS VLLSGRPGCG
560 570 580 590 600
KSSLAATLAK SSEFPFTRII SPNDLLGYNE SAKASKITKV FEDSYKSPMS
610 620 630 640 650
CVVVDEIERL IEYVPIGPRF SNLILQTLAV LFKRTPPKGR KLLVIATTSN
660 670 680 690 700
PDILKDMDIM DCFATVLSVP SISTAKEFQT VCFELGFTQK EASEAASFFT
710 720 730
SPITIKQIIM IVEMARQEEG NFIDNFKMCL EDFNIRNF
Length:738
Mass (Da):82,233
Last modified:July 5, 2004 - v1
Checksum:iCD1AF65CF49BDB64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501F → L in AAC48226 (PubMed:9511743).Curated1
Sequence conflicti592E → D in AAC48226 (PubMed:9511743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006826 mRNA. Translation: AAC48226.1.
AAFI02000014 Genomic DNA. Translation: EAL69376.1.
RefSeqiXP_643273.1. XM_638181.1.

Genome annotation databases

EnsemblProtistsiEAL69376; EAL69376; DDB_G0276153.
GeneIDi8620316.
KEGGiddi:DDB_G0276153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006826 mRNA. Translation: AAC48226.1.
AAFI02000014 Genomic DNA. Translation: EAL69376.1.
RefSeqiXP_643273.1. XM_638181.1.

3D structure databases

ProteinModelPortaliQ75JI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ75JI3. 3 interactors.
STRINGi44689.DDB0185052.

Proteomic databases

PaxDbiQ75JI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL69376; EAL69376; DDB_G0276153.
GeneIDi8620316.
KEGGiddi:DDB_G0276153.

Organism-specific databases

dictyBaseiDDB_G0276153. nsfA.

Phylogenomic databases

eggNOGiKOG0741. Eukaryota.
COG0464. LUCA.
InParanoidiQ75JI3.
KOiK06027.
OMAiFDSADIN.
PhylomeDBiQ75JI3.

Enzyme and pathway databases

ReactomeiR-DDI-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-DDI-6807878. COPI-mediated anterograde transport.
R-DDI-6811438. Intra-Golgi traffic.

Miscellaneous databases

PROiQ75JI3.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSF_DICDI
AccessioniPrimary (citable) accession number: Q75JI3
Secondary accession number(s): O15646, Q552E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.