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Q75JI3

- NSF_DICDI

UniProt

Q75JI3 - NSF_DICDI

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Protein

Vesicle-fusing ATPase

Gene

nsfA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Involved in endocytosis and endosome-endosome fusion. May be required for transport from the endoplasmic reticulum to the Golgi stack, and for the fusion of transport vesicles within the Golgi cisternae. Required for cell polarity, locomotion and chemotaxis.7 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi507 – 5126ATPBy similarity
Nucleotide bindingi547 – 5548ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular component movement Source: dictyBase
  2. chemotaxis Source: UniProtKB-KW
  3. endocytosis Source: dictyBase
  4. phagocytosis Source: dictyBase
  5. protein secretion Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Chemotaxis, ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive factor A
NEM-sensitive fusion protein A
Vesicular-fusion protein nfsA
Gene namesi
Name:nsfA
ORF Names:DDB_G0276153
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276153. nsfA.

Subcellular locationi

Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. endosome Source: UniProtKB-KW
  3. membrane Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738Vesicle-fusing ATPasePRO_0000327890Add
BLAST

Proteomic databases

PRIDEiQ75JI3.

Expressioni

Developmental stagei

Expressed in relatively constant amounts throughout the entire differentiation cycle.1 Publication

Interactioni

Subunit structurei

Interacts with syn7A, snpA and snpC.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
snpAQ54NP62EBI-1810142,EBI-1810173
snpCQ9U9R72EBI-1810142,EBI-1810184
syn7AQ54JY72EBI-1810142,EBI-1810238

Protein-protein interaction databases

IntActiQ75JI3. 3 interactions.
STRINGi44689.DDB_0185052.

Structurei

3D structure databases

ProteinModelPortaliQ75JI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
InParanoidiQ75JI3.
KOiK06027.
OMAiMTIELEF.
PhylomeDBiQ75JI3.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75JI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYPPPVGDS IKLKVQASND PEEAFTNRAY LPISSFSFLF PNVQSNLYTT
60 70 80 90 100
NTNYIKIRVG ANEYILSASP NKNMKPDSIA LSKALRGWMY VSNNEEVYVE
110 120 130 140 150
FYDPNPNICG SMKVSIDYLT KGKQGPKQDS QEIIGKIIDN FNSQYFTFGQ
160 170 180 190 200
LFYIKNSNST FELRVEAVET NEVPTKDKGW AIISPATKII LQKMPGSLID
210 220 230 240 250
IETNGPLVVN QIFTSDWDFE NMGIGGLDAE FRDIFRRAFS SRIFPPAIVK
260 270 280 290 300
KLGVNHVKGM LLYGPPGTGK TLIARQIGKM LNGREPKVVS GPSILNKYVG
310 320 330 340 350
QSEENIRMLF RDAEIEQKAK GDDSGLHIII FDELDAICKS RGSRQGDSGV
360 370 380 390 400
GDSVVNQLLA MIDGVESLNN ILVIGMTNRK DMIDEALLRP GRLEVHVEIS
410 420 430 440 450
LPDEHGREQI FKIHTAKMRD QNALDKDVNL ANYAHTTRNY SGAEIEGVVK
460 470 480 490 500
SAASYAFSRQ VDTKNIKNVE IKPEDIKVCD QDFKRAITEV TPSFGSTDNQ
510 520 530 540 550
FESYAENGII NYGPVFDKLL QSGNAFVEQV KKSNRTPMMS VLLSGRPGCG
560 570 580 590 600
KSSLAATLAK SSEFPFTRII SPNDLLGYNE SAKASKITKV FEDSYKSPMS
610 620 630 640 650
CVVVDEIERL IEYVPIGPRF SNLILQTLAV LFKRTPPKGR KLLVIATTSN
660 670 680 690 700
PDILKDMDIM DCFATVLSVP SISTAKEFQT VCFELGFTQK EASEAASFFT
710 720 730
SPITIKQIIM IVEMARQEEG NFIDNFKMCL EDFNIRNF
Length:738
Mass (Da):82,233
Last modified:July 5, 2004 - v1
Checksum:iCD1AF65CF49BDB64
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti501 – 5011F → L in AAC48226. (PubMed:9511743)Curated
Sequence conflicti592 – 5921E → D in AAC48226. (PubMed:9511743)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006826 mRNA. Translation: AAC48226.1.
AAFI02000014 Genomic DNA. Translation: EAL69376.1.
RefSeqiXP_643273.1. XM_638181.1.

Genome annotation databases

EnsemblProtistsiDDB0185052; DDB0185052; DDB_G0276153.
GeneIDi8620316.
KEGGiddi:DDB_G0276153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006826 mRNA. Translation: AAC48226.1 .
AAFI02000014 Genomic DNA. Translation: EAL69376.1 .
RefSeqi XP_643273.1. XM_638181.1.

3D structure databases

ProteinModelPortali Q75JI3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q75JI3. 3 interactions.
STRINGi 44689.DDB_0185052.

Proteomic databases

PRIDEi Q75JI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185052 ; DDB0185052 ; DDB_G0276153 .
GeneIDi 8620316.
KEGGi ddi:DDB_G0276153.

Organism-specific databases

dictyBasei DDB_G0276153. nsfA.

Phylogenomic databases

eggNOGi COG0464.
InParanoidi Q75JI3.
KOi K06027.
OMAi MTIELEF.
PhylomeDBi Q75JI3.

Miscellaneous databases

PROi Q75JI3.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein."
    Weidenhaupt M., Bruckert F., Satre M.
    Gene 207:53-60(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: AX2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Characterization of endosome-endosome fusion in a cell-free system using Dictyostelium discoideum."
    Lenhard J.M., Mayorga L., Stahl P.D.
    J. Biol. Chem. 267:1896-1903(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "In vitro reconstituted Dictyostelium discoideum early endosome fusion is regulated by Rab7 but proceeds in the absence of ATP-Mg2+ from the bulk solution."
    Laurent O., Bruckert F., Adessi C., Satre M.
    J. Biol. Chem. 273:793-799(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Functional and molecular identification of novel members of the ubiquitous membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-sensitive factor-attachment proteins) families in Dictyostelium discoideum."
    Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M.
    Eur. J. Biochem. 267:2062-2070(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SNPA AND SNPC.
  7. "A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion."
    Bogdanovic A., Bruckert F., Morio T., Satre M.
    J. Biol. Chem. 275:36691-36697(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SYN7A.
  8. "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum."
    Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J., Satre M., Bruckert F.
    Biochem. J. 368:29-39(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SYN7A, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Cell polarity and locomotion, as well as endocytosis, depend on NSF."
    Thompson C.R.L., Bretscher M.S.
    Development 129:4185-4192(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Possible roles of the endocytic cycle in cell motility."
    Traynor D., Kay R.R.
    J. Cell Sci. 120:2318-2327(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNSF_DICDI
AccessioniPrimary (citable) accession number: Q75JI3
Secondary accession number(s): O15646, Q552E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3