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Protein

Beta-glucosidase 8

Gene

BGLU8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides, sophorose and gentiobiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
  2. KM=1.6 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
  3. KM=26 mM for cellobiose (at pH 5.0)1 Publication
  4. KM=0.56 mM for cellotriose (at pH 5.0)1 Publication
  5. KM=0.25 mM for cellotetraose (at pH 5.0)1 Publication
  6. KM=0.15 mM for cellopentaose (at pH 5.0)1 Publication
  7. KM=0.12 mM for cellohexaose (at pH 5.0)1 Publication
  8. KM=0.32 mM for laminaribiose (at pH 5.0)1 Publication
  9. KM=6.0 mM for laminaritriose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity
    Binding sitei210 – 2101SubstrateBy similarity
    Active sitei211 – 2111Proton donorBy similarity
    Binding sitei350 – 3501SubstrateBy similarity
    Active sitei421 – 4211NucleophileBy similarity
    Binding sitei468 – 4681SubstrateBy similarity

    GO - Molecular functioni

    • beta-D-fucosidase activity Source: UniProtKB
    • beta-galactosidase activity Source: UniProtKB
    • beta-gentiobiose beta-glucosidase activity Source: UniProtKB
    • beta-glucosidase activity Source: UniProtKB
    • beta-L-arabinosidase activity Source: UniProtKB
    • beta-mannosidase activity Source: UniProtKB
    • cellobiose glucosidase activity Source: UniProtKB
    • glucan exo-1,3-beta-glucosidase activity Source: UniProtKB
    • scopolin beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ75I94.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 8 (EC:3.2.1.21)
    Short name:
    Os3bglu8
    Gene namesi
    Name:BGLU8
    Ordered Locus Names:Os03g0703100, LOC_Os03g49610
    ORF Names:OsJ_12263, OSJNBa0004L11.15
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
    Proteomesi
    • UP000059680 Componenti: Chromosome 3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence analysisAdd
    BLAST
    Chaini34 – 568535Beta-glucosidase 8PRO_0000390325Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi230 ↔ 233By similarity
    Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ75I94.
    PRIDEiQ75I94.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ75I94. baseline and differential.
    GenevisibleiQ75I94. OS.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os03g49610.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75I94.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni475 – 4762Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG0626. Eukaryota.
    COG2723. LUCA.
    InParanoidiQ75I94.
    OMAiYDAYLMP.
    OrthoDBiEOG093606U3.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q75I94-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGCAPAAHYL PGGGWRRLLV VVVALVVLDR AGARVRAADD DTGGLSRAAF
    60 70 80 90 100
    PKGFVFGTAT SAFQVEGMAA SGGRGPSIWD PFVHTPGNIA GNGNADVTTD
    110 120 130 140 150
    EYHRYKEDVD LLKSLNFDAY RFSISWSRIF PDGEGKVNTE GVAYYNNLID
    160 170 180 190 200
    YVIKQGLIPY VNLNHYDLPL ALQKKYEGWL SPKIVGVFSD YAEFCFKTYG
    210 220 230 240 250
    DRVKNWFTFN EPRIVAALGH DTGTDPPNRC TKCAAGGNSA TEPYIVAHNI
    260 270 280 290 300
    ILSHATAVDR YRNKFQASQK GKIGIVLDFN WYEPLTNSTE DQAAAQRARD
    310 320 330 340 350
    FHVGWFLDPL INGQYPKNMR DIVKERLPTF TPEQAKLVKG SADYFGINQY
    360 370 380 390 400
    TANYMADQPA PQQAATSYSS DWHVSFIFQR NGVPIGQQAN SNWLYIVPTG
    410 420 430 440 450
    MYGAVNYIKE KYNNPTIIIS ENGMDQSGNL TREEFLHDTE RIEFYKNYLT
    460 470 480 490 500
    ELKKAIDDGA NVVAYFAWSL LDNFEWLSGY TSKFGIVYVD FTTLKRYPKD
    510 520 530 540 550
    SANWFKNMLQ ASGPGSKSGS GTSDSQVGSA TSASHPVGSA ISSSHRLLLP
    560
    LLVSLHFLFP SFFMFLSL
    Length:568
    Mass (Da):63,098
    Last modified:July 5, 2004 - v1
    Checksum:i67F1C9B4B9E22F6D
    GO

    Sequence cautioni

    The sequence AAX95520 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC091670 Genomic DNA. Translation: AAX95520.1. Sequence problems.
    AC133334 Genomic DNA. Translation: AAS07251.1.
    DP000009 Genomic DNA. Translation: ABF98427.1.
    AP008209 Genomic DNA. Translation: BAF12928.1.
    AP014959 Genomic DNA. Translation: BAS85955.1.
    CM000140 Genomic DNA. Translation: EEE59768.1.
    AK120790 mRNA. Translation: BAH00173.1.
    RefSeqiXP_015630330.1. XM_015774844.1.
    UniGeneiOs.32141.

    Genome annotation databases

    EnsemblPlantsiOS03T0703100-01; OS03T0703100-01; OS03G0703100.
    GeneIDi4333842.
    GrameneiOS03T0703100-01; OS03T0703100-01; OS03G0703100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC091670 Genomic DNA. Translation: AAX95520.1. Sequence problems.
    AC133334 Genomic DNA. Translation: AAS07251.1.
    DP000009 Genomic DNA. Translation: ABF98427.1.
    AP008209 Genomic DNA. Translation: BAF12928.1.
    AP014959 Genomic DNA. Translation: BAS85955.1.
    CM000140 Genomic DNA. Translation: EEE59768.1.
    AK120790 mRNA. Translation: BAH00173.1.
    RefSeqiXP_015630330.1. XM_015774844.1.
    UniGeneiOs.32141.

    3D structure databases

    ProteinModelPortaliQ75I94.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi39947.LOC_Os03g49610.1.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbiQ75I94.
    PRIDEiQ75I94.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiOS03T0703100-01; OS03T0703100-01; OS03G0703100.
    GeneIDi4333842.
    GrameneiOS03T0703100-01; OS03T0703100-01; OS03G0703100.

    Phylogenomic databases

    eggNOGiKOG0626. Eukaryota.
    COG2723. LUCA.
    InParanoidiQ75I94.
    OMAiYDAYLMP.
    OrthoDBiEOG093606U3.

    Enzyme and pathway databases

    SABIO-RKQ75I94.

    Gene expression databases

    ExpressionAtlasiQ75I94. baseline and differential.
    GenevisibleiQ75I94. OS.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBGL08_ORYSJ
    AccessioniPrimary (citable) accession number: Q75I94
    Secondary accession number(s): A0A0P0W1Y4, Q53RI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 5, 2004
    Last modified: September 7, 2016
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.