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Q75I94

- BGL08_ORYSJ

UniProt

Q75I94 - BGL08_ORYSJ

Protein

Beta-glucosidase 8

Gene

BGLU8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides, sophorose and gentiobiose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
    2. KM=1.6 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
    3. KM=26 mM for cellobiose (at pH 5.0)1 Publication
    4. KM=0.56 mM for cellotriose (at pH 5.0)1 Publication
    5. KM=0.25 mM for cellotetraose (at pH 5.0)1 Publication
    6. KM=0.15 mM for cellopentaose (at pH 5.0)1 Publication
    7. KM=0.12 mM for cellohexaose (at pH 5.0)1 Publication
    8. KM=0.32 mM for laminaribiose (at pH 5.0)1 Publication
    9. KM=6.0 mM for laminaritriose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity
    Binding sitei210 – 2101SubstrateBy similarity
    Active sitei211 – 2111Proton donorBy similarity
    Binding sitei350 – 3501SubstrateBy similarity
    Active sitei421 – 4211NucleophileBy similarity
    Binding sitei468 – 4681SubstrateBy similarity

    GO - Molecular functioni

    1. beta-D-fucosidase activity Source: UniProtKB
    2. beta-galactosidase activity Source: UniProtKB
    3. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
    4. beta-glucosidase activity Source: UniProtKB
    5. beta-L-arabinosidase activity Source: UniProtKB
    6. beta-mannosidase activity Source: UniProtKB
    7. cellobiose glucosidase activity Source: UniProtKB
    8. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ75I94.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 8 (EC:3.2.1.21)
    Short name:
    Os3bglu8
    Gene namesi
    Name:BGLU8
    Ordered Locus Names:Os03g0703100, LOC_Os03g49610
    ORF Names:OsJ_12263, OSJNBa0004L11.15
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ75I94.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 568535Beta-glucosidase 8PRO_0000390325Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi230 ↔ 233By similarity
    Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ75I94.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75I94.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni475 – 4762Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    OMAiTDEYHRY.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q75I94-1 [UniParc]FASTAAdd to Basket

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    MGCAPAAHYL PGGGWRRLLV VVVALVVLDR AGARVRAADD DTGGLSRAAF    50
    PKGFVFGTAT SAFQVEGMAA SGGRGPSIWD PFVHTPGNIA GNGNADVTTD 100
    EYHRYKEDVD LLKSLNFDAY RFSISWSRIF PDGEGKVNTE GVAYYNNLID 150
    YVIKQGLIPY VNLNHYDLPL ALQKKYEGWL SPKIVGVFSD YAEFCFKTYG 200
    DRVKNWFTFN EPRIVAALGH DTGTDPPNRC TKCAAGGNSA TEPYIVAHNI 250
    ILSHATAVDR YRNKFQASQK GKIGIVLDFN WYEPLTNSTE DQAAAQRARD 300
    FHVGWFLDPL INGQYPKNMR DIVKERLPTF TPEQAKLVKG SADYFGINQY 350
    TANYMADQPA PQQAATSYSS DWHVSFIFQR NGVPIGQQAN SNWLYIVPTG 400
    MYGAVNYIKE KYNNPTIIIS ENGMDQSGNL TREEFLHDTE RIEFYKNYLT 450
    ELKKAIDDGA NVVAYFAWSL LDNFEWLSGY TSKFGIVYVD FTTLKRYPKD 500
    SANWFKNMLQ ASGPGSKSGS GTSDSQVGSA TSASHPVGSA ISSSHRLLLP 550
    LLVSLHFLFP SFFMFLSL 568
    Length:568
    Mass (Da):63,098
    Last modified:July 5, 2004 - v1
    Checksum:i67F1C9B4B9E22F6D
    GO

    Sequence cautioni

    The sequence AAX95520.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC091670 Genomic DNA. Translation: AAX95520.1. Sequence problems.
    AC133334 Genomic DNA. Translation: AAS07251.1.
    DP000009 Genomic DNA. Translation: ABF98427.1.
    AP008209 Genomic DNA. Translation: BAF12928.1.
    CM000140 Genomic DNA. Translation: EEE59768.1.
    AK120790 mRNA. Translation: BAH00173.1.
    RefSeqiNP_001051014.1. NM_001057549.1.
    UniGeneiOs.32141.

    Genome annotation databases

    EnsemblPlantsiOS03T0703100-01; OS03T0703100-01; OS03G0703100.
    GeneIDi4333842.
    KEGGiosa:4333842.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC091670 Genomic DNA. Translation: AAX95520.1 . Sequence problems.
    AC133334 Genomic DNA. Translation: AAS07251.1 .
    DP000009 Genomic DNA. Translation: ABF98427.1 .
    AP008209 Genomic DNA. Translation: BAF12928.1 .
    CM000140 Genomic DNA. Translation: EEE59768.1 .
    AK120790 mRNA. Translation: BAH00173.1 .
    RefSeqi NP_001051014.1. NM_001057549.1.
    UniGenei Os.32141.

    3D structure databases

    ProteinModelPortali Q75I94.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q75I94.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0703100-01 ; OS03T0703100-01 ; OS03G0703100 .
    GeneIDi 4333842.
    KEGGi osa:4333842.

    Organism-specific databases

    Gramenei Q75I94.

    Phylogenomic databases

    eggNOGi COG2723.
    OMAi TDEYHRY.

    Enzyme and pathway databases

    SABIO-RK Q75I94.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    6. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
      Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
      Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBGL08_ORYSJ
    AccessioniPrimary (citable) accession number: Q75I94
    Secondary accession number(s): Q53RI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3