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Protein

Beta-glucosidase 8

Gene

BGLU8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides, sophorose and gentiobiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
  2. KM=1.6 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
  3. KM=26 mM for cellobiose (at pH 5.0)1 Publication
  4. KM=0.56 mM for cellotriose (at pH 5.0)1 Publication
  5. KM=0.25 mM for cellotetraose (at pH 5.0)1 Publication
  6. KM=0.15 mM for cellopentaose (at pH 5.0)1 Publication
  7. KM=0.12 mM for cellohexaose (at pH 5.0)1 Publication
  8. KM=0.32 mM for laminaribiose (at pH 5.0)1 Publication
  9. KM=6.0 mM for laminaritriose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei64SubstrateBy similarity1
    Binding sitei165SubstrateBy similarity1
    Binding sitei210SubstrateBy similarity1
    Active sitei211Proton donorBy similarity1
    Binding sitei350SubstrateBy similarity1
    Active sitei421NucleophileBy similarity1
    Binding sitei468SubstrateBy similarity1

    GO - Molecular functioni

    • beta-D-fucosidase activity Source: UniProtKB
    • beta-galactosidase activity Source: UniProtKB
    • beta-gentiobiose beta-glucosidase activity Source: UniProtKB
    • beta-glucosidase activity Source: UniProtKB
    • beta-L-arabinosidase activity Source: UniProtKB
    • beta-mannosidase activity Source: UniProtKB
    • cellobiose glucosidase activity Source: UniProtKB
    • glucan exo-1,3-beta-glucosidase activity Source: UniProtKB
    • scopolin beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiR-OSA-189085 Digestion of dietary carbohydrate
    SABIO-RKiQ75I94

    Protein family/group databases

    CAZyiGH1 Glycoside Hydrolase Family 1

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 8 (EC:3.2.1.21)
    Short name:
    Os3bglu8
    Gene namesi
    Name:BGLU8
    Ordered Locus Names:Os03g0703100, LOC_Os03g49610
    ORF Names:OsJ_12263, OSJNBa0004L11.15
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
    Proteomesi
    • UP000059680 Componenti: Chromosome 3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 33Sequence analysisAdd BLAST33
    ChainiPRO_000039032534 – 568Beta-glucosidase 8Add BLAST535

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi230 ↔ 233By similarity
    Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ75I94
    PRIDEiQ75I94

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ75I94 differential
    GenevisibleiQ75I94 OS

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os03g49610.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ75I94
    SMRiQ75I94
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni475 – 476Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG0626 Eukaryota
    COG2723 LUCA
    InParanoidiQ75I94
    OMAiHFLFPSF
    OrthoDBiEOG093606U3

    Family and domain databases

    InterProiView protein in InterPro
    IPR001360 Glyco_hydro_1
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR10353 PTHR10353, 1 hit
    PfamiView protein in Pfam
    PF00232 Glyco_hydro_1, 1 hit
    PRINTSiPR00131 GLHYDRLASE1
    SUPFAMiSSF51445 SSF51445, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q75I94-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGCAPAAHYL PGGGWRRLLV VVVALVVLDR AGARVRAADD DTGGLSRAAF
    60 70 80 90 100
    PKGFVFGTAT SAFQVEGMAA SGGRGPSIWD PFVHTPGNIA GNGNADVTTD
    110 120 130 140 150
    EYHRYKEDVD LLKSLNFDAY RFSISWSRIF PDGEGKVNTE GVAYYNNLID
    160 170 180 190 200
    YVIKQGLIPY VNLNHYDLPL ALQKKYEGWL SPKIVGVFSD YAEFCFKTYG
    210 220 230 240 250
    DRVKNWFTFN EPRIVAALGH DTGTDPPNRC TKCAAGGNSA TEPYIVAHNI
    260 270 280 290 300
    ILSHATAVDR YRNKFQASQK GKIGIVLDFN WYEPLTNSTE DQAAAQRARD
    310 320 330 340 350
    FHVGWFLDPL INGQYPKNMR DIVKERLPTF TPEQAKLVKG SADYFGINQY
    360 370 380 390 400
    TANYMADQPA PQQAATSYSS DWHVSFIFQR NGVPIGQQAN SNWLYIVPTG
    410 420 430 440 450
    MYGAVNYIKE KYNNPTIIIS ENGMDQSGNL TREEFLHDTE RIEFYKNYLT
    460 470 480 490 500
    ELKKAIDDGA NVVAYFAWSL LDNFEWLSGY TSKFGIVYVD FTTLKRYPKD
    510 520 530 540 550
    SANWFKNMLQ ASGPGSKSGS GTSDSQVGSA TSASHPVGSA ISSSHRLLLP
    560
    LLVSLHFLFP SFFMFLSL
    Length:568
    Mass (Da):63,098
    Last modified:July 5, 2004 - v1
    Checksum:i67F1C9B4B9E22F6D
    GO

    Sequence cautioni

    The sequence AAX95520 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC091670 Genomic DNA Translation: AAX95520.1 Sequence problems.
    AC133334 Genomic DNA Translation: AAS07251.1
    DP000009 Genomic DNA Translation: ABF98427.1
    AP008209 Genomic DNA Translation: BAF12928.1
    AP014959 Genomic DNA Translation: BAS85955.1
    CM000140 Genomic DNA Translation: EEE59768.1
    AK120790 mRNA Translation: BAH00173.1
    RefSeqiXP_015630330.1, XM_015774844.1
    UniGeneiOs.32141

    Genome annotation databases

    EnsemblPlantsiOs03t0703100-01; Os03t0703100-01; Os03g0703100
    GeneIDi4333842
    GrameneiOs03t0703100-01; Os03t0703100-01; Os03g0703100
    KEGGiosa:4333842

    Similar proteinsi

    Entry informationi

    Entry nameiBGL08_ORYSJ
    AccessioniPrimary (citable) accession number: Q75I94
    Secondary accession number(s): A0A0P0W1Y4, Q53RI4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 5, 2004
    Last modified: May 23, 2018
    This is version 87 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

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