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Q75I94

- BGL08_ORYSJ

UniProt

Q75I94 - BGL08_ORYSJ

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Protein

Beta-glucosidase 8

Gene

BGLU8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides, sophorose and gentiobiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
  2. KM=1.6 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
  3. KM=26 mM for cellobiose (at pH 5.0)1 Publication
  4. KM=0.56 mM for cellotriose (at pH 5.0)1 Publication
  5. KM=0.25 mM for cellotetraose (at pH 5.0)1 Publication
  6. KM=0.15 mM for cellopentaose (at pH 5.0)1 Publication
  7. KM=0.12 mM for cellohexaose (at pH 5.0)1 Publication
  8. KM=0.32 mM for laminaribiose (at pH 5.0)1 Publication
  9. KM=6.0 mM for laminaritriose (at pH 5.0)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateBy similarity
Binding sitei165 – 1651SubstrateBy similarity
Binding sitei210 – 2101SubstrateBy similarity
Active sitei211 – 2111Proton donorBy similarity
Binding sitei350 – 3501SubstrateBy similarity
Active sitei421 – 4211NucleophileBy similarity
Binding sitei468 – 4681SubstrateBy similarity

GO - Molecular functioni

  1. beta-D-fucosidase activity Source: UniProtKB
  2. beta-galactosidase activity Source: UniProtKB
  3. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
  4. beta-glucosidase activity Source: UniProtKB
  5. beta-L-arabinosidase activity Source: UniProtKB
  6. beta-mannosidase activity Source: UniProtKB
  7. cellobiose glucosidase activity Source: UniProtKB
  8. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ75I94.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 8 (EC:3.2.1.21)
Short name:
Os3bglu8
Gene namesi
Name:BGLU8
Ordered Locus Names:Os03g0703100, LOC_Os03g49610
ORF Names:OsJ_12263, OSJNBa0004L11.15
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ75I94.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 568535Beta-glucosidase 8PRO_0000390325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi230 ↔ 233By similarity
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ75I94.

Structurei

3D structure databases

ProteinModelPortaliQ75I94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni475 – 4762Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
InParanoidiQ75I94.
OMAiTDEYHRY.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75I94-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCAPAAHYL PGGGWRRLLV VVVALVVLDR AGARVRAADD DTGGLSRAAF
60 70 80 90 100
PKGFVFGTAT SAFQVEGMAA SGGRGPSIWD PFVHTPGNIA GNGNADVTTD
110 120 130 140 150
EYHRYKEDVD LLKSLNFDAY RFSISWSRIF PDGEGKVNTE GVAYYNNLID
160 170 180 190 200
YVIKQGLIPY VNLNHYDLPL ALQKKYEGWL SPKIVGVFSD YAEFCFKTYG
210 220 230 240 250
DRVKNWFTFN EPRIVAALGH DTGTDPPNRC TKCAAGGNSA TEPYIVAHNI
260 270 280 290 300
ILSHATAVDR YRNKFQASQK GKIGIVLDFN WYEPLTNSTE DQAAAQRARD
310 320 330 340 350
FHVGWFLDPL INGQYPKNMR DIVKERLPTF TPEQAKLVKG SADYFGINQY
360 370 380 390 400
TANYMADQPA PQQAATSYSS DWHVSFIFQR NGVPIGQQAN SNWLYIVPTG
410 420 430 440 450
MYGAVNYIKE KYNNPTIIIS ENGMDQSGNL TREEFLHDTE RIEFYKNYLT
460 470 480 490 500
ELKKAIDDGA NVVAYFAWSL LDNFEWLSGY TSKFGIVYVD FTTLKRYPKD
510 520 530 540 550
SANWFKNMLQ ASGPGSKSGS GTSDSQVGSA TSASHPVGSA ISSSHRLLLP
560
LLVSLHFLFP SFFMFLSL
Length:568
Mass (Da):63,098
Last modified:July 5, 2004 - v1
Checksum:i67F1C9B4B9E22F6D
GO

Sequence cautioni

The sequence AAX95520.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091670 Genomic DNA. Translation: AAX95520.1. Sequence problems.
AC133334 Genomic DNA. Translation: AAS07251.1.
DP000009 Genomic DNA. Translation: ABF98427.1.
AP008209 Genomic DNA. Translation: BAF12928.1.
CM000140 Genomic DNA. Translation: EEE59768.1.
AK120790 mRNA. Translation: BAH00173.1.
RefSeqiNP_001051014.1. NM_001057549.1.
UniGeneiOs.32141.

Genome annotation databases

EnsemblPlantsiOS03T0703100-01; OS03T0703100-01; OS03G0703100.
GeneIDi4333842.
KEGGiosa:4333842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091670 Genomic DNA. Translation: AAX95520.1 . Sequence problems.
AC133334 Genomic DNA. Translation: AAS07251.1 .
DP000009 Genomic DNA. Translation: ABF98427.1 .
AP008209 Genomic DNA. Translation: BAF12928.1 .
CM000140 Genomic DNA. Translation: EEE59768.1 .
AK120790 mRNA. Translation: BAH00173.1 .
RefSeqi NP_001051014.1. NM_001057549.1.
UniGenei Os.32141.

3D structure databases

ProteinModelPortali Q75I94.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi Q75I94.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0703100-01 ; OS03T0703100-01 ; OS03G0703100 .
GeneIDi 4333842.
KEGGi osa:4333842.

Organism-specific databases

Gramenei Q75I94.

Phylogenomic databases

eggNOGi COG2723.
InParanoidi Q75I94.
OMAi TDEYHRY.

Enzyme and pathway databases

SABIO-RK Q75I94.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
    Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
    BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
    Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
    Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiBGL08_ORYSJ
AccessioniPrimary (citable) accession number: Q75I94
Secondary accession number(s): Q53RI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3