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Q75I93

- BGL07_ORYSJ

UniProt

Q75I93 - BGL07_ORYSJ

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Protein
Beta-glucosidase 7
Gene
BGLU7, BGLU1, Os03g0703000, LOC_Os03g49600, OSJNBa0004L11.16
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.3 Publications

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=0.2 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)3 Publications
  2. KM=0.23 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0)
  3. KM=3.2 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0)
  4. KM=1.3 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)
  5. KM=1.3 mM for p-nitrophenyl beta-D-xyloside (at pH 5.0)
  6. KM=1.2 mM for p-nitrophenyl beta-L-arabinoside (at pH 5.0)
  7. KM=0.78 mM for p-nitrophenyl beta-D-cellobioside (at pH 5.0)
  8. KM=22 mM for cellobiose (at pH 5.0)
  9. KM=0.22 mM for cellotriose (at pH 5.0)
  10. KM=0.28 mM for cellotetraose (at pH 5.0)
  11. KM=0.24 mM for cellopentaose (at pH 5.0)
  12. KM=0.22 mM for cellohexaose (at pH 5.0)
  13. KM=2.05 mM for laminaribiose (at pH 5.0)
  14. KM=1.92 mM for laminaritriose (at pH 5.0)
  15. KM=13.9 mM for sophorose (at pH 5.0)
  16. KM=38.3 mM for gentiobiose (at pH 5.0)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571Substrate
Metal bindingi93 – 931Zinc; shared with dimeric partner
Metal bindingi96 – 961Zinc; shared with dimeric partner
Binding sitei158 – 1581Substrate
Binding sitei203 – 2031Substrate
Active sitei204 – 2041Proton donor By similarity
Binding sitei343 – 3431Substrate
Active sitei414 – 4141Nucleophile By similarity
Binding sitei461 – 4611Substrate

GO - Molecular functioni

  1. 4-methylumbelliferyl-beta-D-glucopyranoside beta-glucosidase activity Source: EnsemblPlants/Gramene
  2. amygdalin beta-glucosidase activity Source: UniProtKB
  3. beta-D-fucosidase activity Source: UniProtKB
  4. beta-L-arabinosidase activity Source: UniProtKB
  5. beta-galactosidase activity Source: UniProtKB
  6. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
  7. beta-glucosidase activity Source: UniProtKB
  8. beta-mannosidase activity Source: UniProtKB
  9. cellobiose glucosidase activity Source: UniProtKB
  10. esculin beta-glucosidase activity Source: EnsemblPlants/Gramene
  11. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB
  12. protein homodimerization activity Source: UniProtKB
  13. prunasin beta-glucosidase activity Source: UniProtKB
  14. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKQ75I93.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 7 (EC:3.2.1.21)
Short name:
Os3bglu7
Gene namesi
Name:BGLU7
Synonyms:BGLU1
Ordered Locus Names:Os03g0703000, LOC_Os03g49600
ORF Names:OSJNBa0004L11.16
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ75I93.

Subcellular locationi

Secreted Reviewed prediction

GO - Cellular componenti

  1. cytosolic ribosome Source: EnsemblPlants/Gramene
  2. extracellular region Source: UniProtKB-SubCell
  3. plant-type cell wall Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071I → V: Increases KM for substrate about 5-fold. 1 Publication
Mutagenesisi218 – 2181N → H: Decreases KM for substrate about 2-fold. 1 Publication
Mutagenesisi273 – 2731N → V: Increases KM for substrate about 5-fold. 1 Publication
Mutagenesisi470 – 4701L → R: No effect on KM. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Chaini27 – 504478Beta-glucosidase 7
PRO_0000383461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi223 ↔ 226 By similarity
Glycosylationi280 – 2801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi422 – 4221N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ75I93.

Interactioni

Subunit structurei

Homodimer. Formation of the homodimer is zinc-dependent. Dimerization does not increase activity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni34 – 374
Helixi40 – 423
Beta strandi48 – 525
Helixi55 – 584
Helixi71 – 766
Helixi83 – 853
Beta strandi88 – 903
Helixi94 – 10714
Beta strandi112 – 1165
Helixi119 – 1224
Beta strandi126 – 1283
Helixi132 – 14716
Beta strandi151 – 1566
Helixi163 – 1697
Helixi171 – 1733
Helixi177 – 19216
Turni193 – 1953
Beta strandi198 – 2036
Helixi205 – 2139
Turni232 – 2343
Helixi235 – 25723
Helixi259 – 2624
Beta strandi265 – 2717
Beta strandi274 – 2818
Helixi282 – 29514
Helixi297 – 3059
Helixi310 – 3167
Helixi317 – 3193
Helixi325 – 3317
Beta strandi336 – 3416
Beta strandi345 – 3495
Helixi361 – 3644
Beta strandi368 – 3736
Beta strandi376 – 3794
Helixi392 – 40413
Beta strandi410 – 4145
Beta strandi419 – 4224
Helixi425 – 4295
Helixi432 – 45019
Beta strandi455 – 4617
Helixi469 – 4746
Beta strandi479 – 4824
Turni484 – 4863
Beta strandi489 – 4913
Helixi493 – 5019

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RGLX-ray2.20A/B29-504[»]
2RGMX-ray1.55A/B29-504[»]
3AHTX-ray2.80A/B29-504[»]
3AHVX-ray1.75A/B29-504[»]
3F4VX-ray1.65A/B29-504[»]
3F5JX-ray1.95A/B29-504[»]
3F5KX-ray1.80A/B29-504[»]
3F5LX-ray1.37A/B29-504[»]
3SCNX-ray2.20A/B29-504[»]
3SCOX-ray1.95A/B29-504[»]
3SCPX-ray2.10A/B29-504[»]
3SCQX-ray2.10A/B29-504[»]
3SCRX-ray1.80A/B29-504[»]
3SCSX-ray1.85A/B29-504[»]
3SCTX-ray1.60A/B29-504[»]
3SCUX-ray1.58A/B29-504[»]
3SCVX-ray2.11A/B29-504[»]
3SCWX-ray1.90A/B29-504[»]
ProteinModelPortaliQ75I93.

Miscellaneous databases

EvolutionaryTraceiQ75I93.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni468 – 4692Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
KOiK05350.
OMAiRDENISC.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75I93-1 [UniParc]FASTAAdd to Basket

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MAARRANCAL VLVLALALLA ARDAGAAAVP KPNWLGGLSR AAFPKRFVFG    50
TATSAYQVEG MAASGGRGPS IWDAFAHTPG NVAGNQNGDV ATDQYHRYKE 100
DVNLMKSLNF DAYRFSISWS RIFPDGEGRV NQEGVAYYNN LINYLLQKGI 150
TPYVNLYHYD LPLALEKKYG GWLNAKMADL FTEYADFCFK TFGNRVKHWF 200
TFNEPRIVAL LGYDQGTNPP KRCTKCAAGG NSATEPYIVA HNFLLSHAAA 250
VARYRTKYQA AQQGKVGIVL DFNWYEALSN STEDQAAAQR ARDFHIGWYL 300
DPLINGHYPQ IMQDLVKDRL PKFTPEQARL VKGSADYIGI NQYTASYMKG 350
QQLMQQTPTS YSADWQVTYV FAKNGKPIGP QANSNWLYIV PWGMYGCVNY 400
IKQKYGNPTV VITENGMDQP ANLSRDQYLR DTTRVHFYRS YLTQLKKAID 450
EGANVAGYFA WSLLDNFEWL SGYTSKFGIV YVDFNTLERH PKASAYWFRD 500
MLKH 504
Length:504
Mass (Da):56,872
Last modified:July 5, 2004 - v1
Checksum:i4C36D2A5AF452CE9
GO

Sequence cautioni

The sequence AAS07255.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence ABF98424.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence ABF98425.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence ABF98426.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521A → V in AAA84906. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28047 mRNA. Translation: AAA84906.3.
AC091670 Genomic DNA. Translation: AAX95519.1.
AC133334 Genomic DNA. Translation: AAS07254.1.
AC133334 Genomic DNA. Translation: AAS07255.1. Sequence problems.
DP000009 Genomic DNA. Translation: ABF98423.1.
DP000009 Genomic DNA. Translation: ABF98424.1. Sequence problems.
DP000009 Genomic DNA. Translation: ABF98425.1. Sequence problems.
DP000009 Genomic DNA. Translation: ABF98426.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF12927.1.
AK100165 mRNA. Translation: BAG94472.1.
PIRiT03296.
RefSeqiNP_001051013.1. NM_001057548.1.
UniGeneiOs.5072.

Genome annotation databases

EnsemblPlantsiOS03T0703000-01; OS03T0703000-01; OS03G0703000.
GeneIDi4333841.
KEGGiosa:4333841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28047 mRNA. Translation: AAA84906.3 .
AC091670 Genomic DNA. Translation: AAX95519.1 .
AC133334 Genomic DNA. Translation: AAS07254.1 .
AC133334 Genomic DNA. Translation: AAS07255.1 . Sequence problems.
DP000009 Genomic DNA. Translation: ABF98423.1 .
DP000009 Genomic DNA. Translation: ABF98424.1 . Sequence problems.
DP000009 Genomic DNA. Translation: ABF98425.1 . Sequence problems.
DP000009 Genomic DNA. Translation: ABF98426.1 . Sequence problems.
AP008209 Genomic DNA. Translation: BAF12927.1 .
AK100165 mRNA. Translation: BAG94472.1 .
PIRi T03296.
RefSeqi NP_001051013.1. NM_001057548.1.
UniGenei Os.5072.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2RGL X-ray 2.20 A/B 29-504 [» ]
2RGM X-ray 1.55 A/B 29-504 [» ]
3AHT X-ray 2.80 A/B 29-504 [» ]
3AHV X-ray 1.75 A/B 29-504 [» ]
3F4V X-ray 1.65 A/B 29-504 [» ]
3F5J X-ray 1.95 A/B 29-504 [» ]
3F5K X-ray 1.80 A/B 29-504 [» ]
3F5L X-ray 1.37 A/B 29-504 [» ]
3SCN X-ray 2.20 A/B 29-504 [» ]
3SCO X-ray 1.95 A/B 29-504 [» ]
3SCP X-ray 2.10 A/B 29-504 [» ]
3SCQ X-ray 2.10 A/B 29-504 [» ]
3SCR X-ray 1.80 A/B 29-504 [» ]
3SCS X-ray 1.85 A/B 29-504 [» ]
3SCT X-ray 1.60 A/B 29-504 [» ]
3SCU X-ray 1.58 A/B 29-504 [» ]
3SCV X-ray 2.11 A/B 29-504 [» ]
3SCW X-ray 1.90 A/B 29-504 [» ]
ProteinModelPortali Q75I93.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi Q75I93.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0703000-01 ; OS03T0703000-01 ; OS03G0703000 .
GeneIDi 4333841.
KEGGi osa:4333841.

Organism-specific databases

Gramenei Q75I93.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
KOi K05350.
OMAi RDENISC.

Enzyme and pathway databases

SABIO-RK Q75I93.

Miscellaneous databases

EvolutionaryTracei Q75I93.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Oryza sativa beta-glucosidase mRNA."
    Esen A., Opassiri R., Ketudat Cairns J.R., Akiyama T.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Root.
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Beta-glucosidase, exo-beta-glucanase and pyridoxine transglucosylase activities of rice BGlu1."
    Opassiri R., Hua Y., Wara-Aswapati O., Akiyama T., Svasti J., Esen A., Ketudat Cairns J.R.
    Biochem. J. 379:125-131(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
    Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
    BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
    Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
    Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  9. "Structural insights into rice BGlu1 beta-glucosidase oligosaccharide hydrolysis and transglycosylation."
    Chuenchor W., Pengthaisong S., Robinson R.C., Yuvaniyama J., Oonanant W., Bevan D.R., Esen A., Chen C.J., Opassiri R., Svasti J., Cairns J.R.
    J. Mol. Biol. 377:1200-1215(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 29-504 IN COMPLEX WITH ZINC IONS AND SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ILE-207; ASN-218; ASN-273 AND LEU-470.

Entry informationi

Entry nameiBGL07_ORYSJ
AccessioniPrimary (citable) accession number: Q75I93
Secondary accession number(s): Q10EB0
, Q10EB1, Q10EB2, Q42975, Q75I92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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