Reviewed, UniProtKB/Swiss-Prot Q75HE6 (MTHR_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Ordered Locus Names:	Os03g0815200, LOC_Os03g60090
ORF Names:	OSJNBa0024F18.2, OSJNBb0042K11.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch By similarity.
Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.
Sequence caution	The sequence AK121351 differs from that shown. Reason: Frameshift at position 448.

Ontologies

Keywords
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Probable methylenetetrahydrofolate reductase		PRO_0000190252
Experimental info
	Sequence conflict	5	1	E → G in AK121351. Ref.2
	Sequence conflict	404	1	G → R in AK099527. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q75HE6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C576C748AA847A18	FASTA	594	66,431
	10 20 30 40 50 60 MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVAGGFAC ALDLVQHIRA KYGDYFGITV AGYPEAHPDA IQSTEGATPE AYSNDLAYLK 190 200 210 220 230 240 QKVDAGADLI ITQLFYDTDI FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK 250 260 270 280 290 300 IPAEITAALE PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL 310 320 330 340 350 360 GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP HGRWGDSRNP 370 380 390 400 410 420 SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN FCQGKLTSSP WSELDGLQPE 430 440 450 460 470 480 TKIIDDQLVK INQKGFLTIN SQPAVNGERS DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD 490 500 510 520 530 540 QLIEKSKAFP SLTYIAVNKD GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK 550 560 570 580 590 DEAFEIWSKG WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI

References

[1]

"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC135594 Genomic DNA. Translation: AAR89836.1. AC139172 Genomic DNA. Translation: AAR01748.1. AK062082 mRNA. No translation available. AK099527 mRNA. No translation available. AK121351 mRNA. No translation available.
RefSeq	NP_001051683.1.
UniGene	Os.7657
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Genome annotation databases
GeneID	4334554.
KEGG	osa:4334554.
Organism-specific databases
Gramene	Q75HE6.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR_ORYSJ

Accession

Primary (citable) accession number: Q75HE6
Secondary accession number(s): Q75GR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)