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Q75HE6

- MTHR_ORYSJ

UniProt

Q75HE6 - MTHR_ORYSJ

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Protein

Probable methylenetetrahydrofolate reductase

Gene

Os03g0815200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptorBy similarity
Binding sitei81 – 811FADBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei175 – 1751FADBy similarity
Binding sitei182 – 1821FADBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei285 – 2851SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NADBy similarity
Nucleotide bindingi52 – 532NAD and FADBy similarity
Nucleotide bindingi111 – 1133FADBy similarity
Nucleotide bindingi157 – 1604FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiREACT_247078. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Ordered Locus Names:Os03g0815200, LOC_Os03g60090
ORF Names:OSJNBa0024F18.2, OSJNBb0042K11.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ75HE6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Probable methylenetetrahydrofolate reductasePRO_0000190252Add
BLAST

Proteomic databases

PaxDbiQ75HE6.
PRIDEiQ75HE6.

Expressioni

Gene expression databases

ExpressionAtlasiQ75HE6. baseline.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os03g60090.1.

Structurei

3D structure databases

ProteinModelPortaliQ75HE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiQ75HE6.
KOiK00297.
OMAiYLEFFVS.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q75HE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD
60 70 80 90 100
ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVAGGFAC ALDLVQHIRA KYGDYFGITV
160 170 180 190 200
AGYPEAHPDA IQSTEGATPE AYSNDLAYLK QKVDAGADLI ITQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPAEITAALE
260 270 280 290 300
PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL
310 320 330 340 350
GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP
360 370 380 390 400
HGRWGDSRNP SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN
410 420 430 440 450
FCQGKLTSSP WSELDGLQPE TKIIDDQLVK INQKGFLTIN SQPAVNGERS
460 470 480 490 500
DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD QLIEKSKAFP SLTYIAVNKD
510 520 530 540 550
GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK DEAFEIWSKG
560 570 580 590
WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI
Length:594
Mass (Da):66,431
Last modified:July 5, 2004 - v1
Checksum:iC576C748AA847A18
GO

Sequence cautioni

The sequence AK121351 differs from that shown. Reason: Frameshift at position 448. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51E → G in AK121351. (PubMed:12869764)Curated
Sequence conflicti404 – 4041G → R in AK099527. (PubMed:12869764)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC135594 Genomic DNA. Translation: AAR89836.1.
AC139172 Genomic DNA. Translation: AAR01748.1.
AK062082 mRNA. No translation available.
AK099527 mRNA. No translation available.
AK121351 mRNA. No translation available.
RefSeqiNP_001051683.1. NM_001058218.1.
UniGeneiOs.7657.

Genome annotation databases

EnsemblPlantsiOS03T0815200-01; OS03T0815200-01; OS03G0815200.
GeneIDi4334554.
KEGGiosa:4334554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC135594 Genomic DNA. Translation: AAR89836.1 .
AC139172 Genomic DNA. Translation: AAR01748.1 .
AK062082 mRNA. No translation available.
AK099527 mRNA. No translation available.
AK121351 mRNA. No translation available.
RefSeqi NP_001051683.1. NM_001058218.1.
UniGenei Os.7657.

3D structure databases

ProteinModelPortali Q75HE6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os03g60090.1.

Proteomic databases

PaxDbi Q75HE6.
PRIDEi Q75HE6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0815200-01 ; OS03T0815200-01 ; OS03G0815200 .
GeneIDi 4334554.
KEGGi osa:4334554.

Organism-specific databases

Gramenei Q75HE6.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
InParanoidi Q75HE6.
KOi K00297.
OMAi YLEFFVS.

Enzyme and pathway databases

UniPathwayi UPA00193 .
Reactomei REACT_247078. Metabolism of folate and pterines.

Gene expression databases

ExpressionAtlasi Q75HE6. baseline.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiMTHR_ORYSJ
AccessioniPrimary (citable) accession number: Q75HE6
Secondary accession number(s): Q75GR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3