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Protein

Probable methylenetetrahydrofolate reductase

Gene

Os03g0815200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine (By similarity).By similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei21Proton donor/acceptorBy similarity1
Binding sitei81FADBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei153FADBy similarity1
Binding sitei175FADBy similarity1
Binding sitei182FADBy similarity1
Binding sitei193SubstrateBy similarity1
Binding sitei285SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 26NADBy similarity6
Nucleotide bindingi52 – 53NAD and FADBy similarity2
Nucleotide bindingi111 – 113FADBy similarity3
Nucleotide bindingi157 – 160FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-OSA-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Ordered Locus Names:Os03g0815200, LOC_Os03g60090
ORF Names:OSJNBa0024F18.2, OSJNBb0042K11.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902521 – 594Probable methylenetetrahydrofolate reductaseAdd BLAST594

Proteomic databases

PaxDbiQ75HE6

Expressioni

Gene expression databases

ExpressionAtlasiQ75HE6 differential
GenevisibleiQ75HE6 OS

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os03g60090.1

Structurei

3D structure databases

ProteinModelPortaliQ75HE6
SMRiQ75HE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0564 Eukaryota
COG0685 LUCA
HOGENOMiHOG000246234
InParanoidiQ75HE6
KOiK00297
OMAiMESGDKW
OrthoDBiEOG093604CD

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit

Sequencei

Sequence statusi: Complete.

Q75HE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD
60 70 80 90 100
ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVAGGFAC ALDLVQHIRA KYGDYFGITV
160 170 180 190 200
AGYPEAHPDA IQSTEGATPE AYSNDLAYLK QKVDAGADLI ITQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPAEITAALE
260 270 280 290 300
PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL
310 320 330 340 350
GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP
360 370 380 390 400
HGRWGDSRNP SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN
410 420 430 440 450
FCQGKLTSSP WSELDGLQPE TKIIDDQLVK INQKGFLTIN SQPAVNGERS
460 470 480 490 500
DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD QLIEKSKAFP SLTYIAVNKD
510 520 530 540 550
GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK DEAFEIWSKG
560 570 580 590
WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI
Length:594
Mass (Da):66,431
Last modified:July 5, 2004 - v1
Checksum:iC576C748AA847A18
GO

Sequence cautioni

The sequence AK121351 differs from that shown. Reason: Frameshift at position 448.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5E → G in AK121351 (PubMed:12869764).Curated1
Sequence conflicti404G → R in AK099527 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC135594 Genomic DNA Translation: AAR89836.1
AC139172 Genomic DNA Translation: AAR01748.1
AP014959 Genomic DNA Translation: BAS87029.1
AK062082 mRNA No translation available.
AK099527 mRNA No translation available.
AK121351 mRNA No translation available.
RefSeqiXP_015630556.1, XM_015775070.1
UniGeneiOs.7657

Genome annotation databases

EnsemblPlantsiOs03t0815200-01; Os03t0815200-01; Os03g0815200
GeneIDi4334554
GrameneiOs03t0815200-01; Os03t0815200-01; Os03g0815200
KEGGiosa:4334554

Similar proteinsi

Entry informationi

Entry nameiMTHR_ORYSJ
AccessioniPrimary (citable) accession number: Q75HE6
Secondary accession number(s): Q75GR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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