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Q75HE6

- MTHR_ORYSJ

UniProt

Q75HE6 - MTHR_ORYSJ

Protein

Probable methylenetetrahydrofolate reductase

Gene

Os03g0815200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.By similarity

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211Proton donor/acceptorBy similarity
    Binding sitei81 – 811FADBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei175 – 1751FADBy similarity
    Binding sitei182 – 1821FADBy similarity
    Binding sitei193 – 1931SubstrateBy similarity
    Binding sitei285 – 2851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 266NADBy similarity
    Nucleotide bindingi52 – 532NAD and FADBy similarity
    Nucleotide bindingi111 – 1133FADBy similarity
    Nucleotide bindingi157 – 1604FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine metabolic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Ordered Locus Names:Os03g0815200, LOC_Os03g60090
    ORF Names:OSJNBa0024F18.2, OSJNBb0042K11.1
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ75HE6.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 594594Probable methylenetetrahydrofolate reductasePRO_0000190252Add
    BLAST

    Proteomic databases

    PaxDbiQ75HE6.
    PRIDEiQ75HE6.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi39947.LOC_Os03g60090.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75HE6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    KOiK00297.
    OMAiYLEFFVS.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q75HE6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD    50
    ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK 100
    SNGIQNVLAL RGDPPHGQDK FVQVAGGFAC ALDLVQHIRA KYGDYFGITV 150
    AGYPEAHPDA IQSTEGATPE AYSNDLAYLK QKVDAGADLI ITQLFYDTDI 200
    FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPAEITAALE 250
    PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL 300
    GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP 350
    HGRWGDSRNP SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN 400
    FCQGKLTSSP WSELDGLQPE TKIIDDQLVK INQKGFLTIN SQPAVNGERS 450
    DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD QLIEKSKAFP SLTYIAVNKD 500
    GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK DEAFEIWSKG 550
    WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI 594
    Length:594
    Mass (Da):66,431
    Last modified:July 5, 2004 - v1
    Checksum:iC576C748AA847A18
    GO

    Sequence cautioni

    The sequence AK121351 differs from that shown. Reason: Frameshift at position 448.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51E → G in AK121351. (PubMed:12869764)Curated
    Sequence conflicti404 – 4041G → R in AK099527. (PubMed:12869764)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC135594 Genomic DNA. Translation: AAR89836.1.
    AC139172 Genomic DNA. Translation: AAR01748.1.
    AK062082 mRNA. No translation available.
    AK099527 mRNA. No translation available.
    AK121351 mRNA. No translation available.
    RefSeqiNP_001051683.1. NM_001058218.1.
    UniGeneiOs.7657.

    Genome annotation databases

    EnsemblPlantsiOS03T0815200-01; OS03T0815200-01; OS03G0815200.
    GeneIDi4334554.
    KEGGiosa:4334554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC135594 Genomic DNA. Translation: AAR89836.1 .
    AC139172 Genomic DNA. Translation: AAR01748.1 .
    AK062082 mRNA. No translation available.
    AK099527 mRNA. No translation available.
    AK121351 mRNA. No translation available.
    RefSeqi NP_001051683.1. NM_001058218.1.
    UniGenei Os.7657.

    3D structure databases

    ProteinModelPortali Q75HE6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os03g60090.1.

    Proteomic databases

    PaxDbi Q75HE6.
    PRIDEi Q75HE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0815200-01 ; OS03T0815200-01 ; OS03G0815200 .
    GeneIDi 4334554.
    KEGGi osa:4334554.

    Organism-specific databases

    Gramenei Q75HE6.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    KOi K00297.
    OMAi YLEFFVS.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiMTHR_ORYSJ
    AccessioniPrimary (citable) accession number: Q75HE6
    Secondary accession number(s): Q75GR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3