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Q75HE6 (MTHR_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Ordered Locus Names:Os03g0815200, LOC_Os03g60090
ORF Names:OSJNBa0024F18.2, OSJNBb0042K11.1
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length594 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch By similarity.

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Enzyme regulation

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Sequence caution

The sequence AK121351 differs from that shown. Reason: Frameshift at position 448.

Ontologies

Keywords
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmethionine metabolic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionmethylenetetrahydrofolate reductase (NAD(P)H) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 594594Probable methylenetetrahydrofolate reductase
PRO_0000190252

Regions

Nucleotide binding21 – 266NAD By similarity
Nucleotide binding52 – 532NAD and FAD By similarity
Nucleotide binding111 – 1133FAD By similarity
Nucleotide binding157 – 1604FAD By similarity

Sites

Active site211Proton donor/acceptor By similarity
Binding site811FAD By similarity
Binding site1131Substrate By similarity
Binding site1531FAD By similarity
Binding site1751FAD By similarity
Binding site1821FAD By similarity
Binding site1931Substrate By similarity
Binding site2851Substrate By similarity

Experimental info

Sequence conflict51E → G in AK121351. Ref.2
Sequence conflict4041G → R in AK099527. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q75HE6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C576C748AA847A18

FASTA59466,431
        10         20         30         40         50         60 
MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD ITWGAGGSTA 

        70         80         90        100        110        120 
DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK SNGIQNVLAL RGDPPHGQDK 

       130        140        150        160        170        180 
FVQVAGGFAC ALDLVQHIRA KYGDYFGITV AGYPEAHPDA IQSTEGATPE AYSNDLAYLK 

       190        200        210        220        230        240 
QKVDAGADLI ITQLFYDTDI FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK 

       250        260        270        280        290        300 
IPAEITAALE PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL 

       310        320        330        340        350        360 
GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP HGRWGDSRNP 

       370        380        390        400        410        420 
SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN FCQGKLTSSP WSELDGLQPE 

       430        440        450        460        470        480 
TKIIDDQLVK INQKGFLTIN SQPAVNGERS DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD 

       490        500        510        520        530        540 
QLIEKSKAFP SLTYIAVNKD GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK 

       550        560        570        580        590 
DEAFEIWSKG WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC135594 Genomic DNA. Translation: AAR89836.1.
AC139172 Genomic DNA. Translation: AAR01748.1.
AK062082 mRNA. No translation available.
AK099527 mRNA. No translation available.
AK121351 mRNA. No translation available.
RefSeqNP_001051683.1. NM_001058218.1.
UniGeneOs.7657.

3D structure databases

ProteinModelPortalQ75HE6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os03g60090.1.

Proteomic databases

PaxDbQ75HE6.
PRIDEQ75HE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0815200-01; OS03T0815200-01; OS03G0815200.
GeneID4334554.
KEGGosa:4334554.

Organism-specific databases

GrameneQ75HE6.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246234.
KOK00297.
OMAYLEFFVS.
ProtClustDBPLN02540.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

InterProIPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00677. fadh2_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTHR_ORYSJ
AccessionPrimary (citable) accession number: Q75HE6
Secondary accession number(s): Q75GR7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways