Q75GT3 (CLPB2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein ClpB2, chloroplastic

Alternative name(s):
ATP-dependent Clp protease ATP-binding subunit ClpB homolog 2
Casein lytic proteinase B2

Gene names

Name:	CLPB2
Synonyms:	CLPB-C
Ordered Locus Names:	Os03g0426900, LOC_Os03g31300
ORF Names:	OSJNBa0020H02.1, OSJNBa0083F15.25

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	978 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular chaperone that may play a role in chloroplast development.
Subcellular location	Plastid › chloroplast Ref.5.
Induction	By heat and oxidative stresses. Ref.5
Sequence similarities	Belongs to the clpA/clpB family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chloroplast organization Inferred from electronic annotation. Source: EnsemblPlants/Gramene protein processing Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from direct assay Ref.5. Source: UniProtKB chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast Potential
	Chain	77 – 978	902	Chaperone protein ClpB2, chloroplastic		PRO_0000412577
Regions
	Nucleotide binding	289 – 296	8	ATP Potential
	Nucleotide binding	692 – 699	8	ATP Potential
	Region	244 – 492	249	I By similarity
	Region	618 – 809	192	II By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75GT3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C07DC7C69987083F	FASTA	978	108,985
	10 20 30 40 50 60 MAAAPPLAAG LRPAMAAAQA PVVAAAWGVG ARRGAALSSS ARCRALRLSR GGGGGRDGWV 70 80 90 100 110 120 PPPVVGRMPP RTLSVRCAAS NGRITQQEFT EMAWQSIVSS PEVAKESKHQ IVETEHLMKS 130 140 150 160 170 180 LLEQRNGLAR RIFSKAGVDN TRLLDATEKF IQRQPKVLGE DPGSMLGRDL EALIQRARDF 190 200 210 220 230 240 KKEYGDSFVS VEHLVLGFAE DKRFGRQLFK DFQITVQSLK TAIESIRGKQ NVIDQDPEGK 250 260 270 280 290 300 YEALDKYGKD LTAMARQGKL DPVIGRDDEI RRCIQILSRR TKNNPVLIGE PGVGKTAIAE 310 320 330 340 350 360 GLAQRIVQGD VPQALTNRRL IALDMGALIA GAKYRGEFED RLKAVLKEVT DSDGQTILFI 370 380 390 400 410 420 DEIHTVVGAG ATNGAMDAGN LLKPMLGRGE LRCIGATTLD EYRKYIEKDP ALERRFQQVY 430 440 450 460 470 480 VDQPSVEDTI SILRGLRERY ELHHGVRISD SALVAAALLS DRYISGRFLP DKAIDLVDES 490 500 510 520 530 540 AAKLKMEITS KPTALDEIDR AVIKLEMERL SLTNDTDKAS RDRLSRIEAE LSLLKEKQKD 550 560 570 580 590 600 LTEQWEREKS VMTKIQSIKE EIDRVNVEIQ QAEREYDLNR AAELKYGSLN ALQRQLQTTE 610 620 630 640 650 660 KELDEYQSSG KSMLREEVTQ DDIAEIVSRW TGIPVSKLKQ SDREKLLYLE EELHKRVVGQ 670 680 690 700 710 720 DPAVKAVSEA IQRSRAGLSD PNRPIASFMF MGPTGVGKTE LAKALAAFMF NTEEAVVRID 730 740 750 760 770 780 MSEYMEKHSV SRLIGAPPGY VGYEEGGQLT EAVRRRPYSI ILFDEIEKAH GDVFNVFLQI 790 800 810 820 830 840 LDDGRVTDSQ GRKVSFTNSI IIMTSNVGSQ FILNMDEEGG STDSAYENIK KRVMDAARSV 850 860 870 880 890 900 FRPEFMNRID EYIVFKPLER EQINSIVKLQ LARVQKRIAD RKIKLEVSPG AVEFLGSLGY 910 920 930 940 950 960 DPNYGARPVK RVIQQYVENE LAKGILRGDF KDEDSILVDT QVTVPSNGQL PQQKLVFHKM 970 SEESAPAAAE DEKFLPAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes." Singh A., Singh U., Mittal D., Grover A. BMC Genomics 11:95-95(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC133398 Genomic DNA. Translation: AAR01771.1. AC137999 Genomic DNA. Translation: AAS07199.1. DP000009 Genomic DNA. Translation: ABF96723.1. AP008209 Genomic DNA. Translation: BAF12324.1. AK069123 mRNA. Translation: BAG91268.1.
RefSeq	NP_001050410.1. NM_001056945.1.
UniGene	Os.7626.
3D structure databases
ProteinModelPortal	Q75GT3.
ModBase	Search...
Proteomic databases
PRIDE	Q75GT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g31300.1; LOC_Os03g31300.1; LOC_Os03g31300.
GeneID	4333161.
KEGG	osa:4333161.
Organism-specific databases
Gramene	Q75GT3.
Phylogenomic databases
HOGENOM	HOG000218211.
KO	K03695.
OMA	NIRDINA.
ProtClustDB	CLSN2688403.
Gene expression databases
ArrayExpress	Q75GT3.
Family and domain databases
Gene3D	1.10.1780.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR018368. Chaperonin_ClpA/B_CS. IPR017730. Chaperonin_ClpB. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. IPR004176. Clp_N. IPR023150. Dbl_Clp-N. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF02861. Clp_N. 2 hits. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
TIGRFAMs	TIGR03346. chaperone_ClpB. 1 hit.
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPB2_ORYSJ

Accession

Primary (citable) accession number: Q75GT3
Secondary accession number(s): Q75I57

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program