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Protein

Deoxyhypusine synthase

Gene

DYS1

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140NADBy similarity1
Binding sitei257NADBy similarity1
Binding sitei262SpermidineBy similarity1
Binding sitei304NAD; via amide nitrogenBy similarity1
Binding sitei309SpermidineBy similarity1
Active sitei350NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 112NADBy similarity5
Nucleotide bindingi134 – 136NADBy similarity3
Nucleotide bindingi329 – 330NADBy similarity2
Nucleotide bindingi363 – 364NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:DYS1
Ordered Locus Names:AAL036W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344801 – 382Deoxyhypusine synthaseAdd BLAST382

Structurei

3D structure databases

ProteinModelPortaliQ75EW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 140Spermidine bindingBy similarity2
Regioni335 – 337Spermidine bindingBy similarity3
Regioni344 – 350Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000228838.
InParanoidiQ75EW4.
KOiK00809.
OMAiHDFTISA.
OrthoDBiEOG092C3JEN.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q75EW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNNDETVLG HLADHVFKHS GPIPESFVEV KGIDYSKPDA IDMRASDLIK
60 70 80 90 100
SMKTMGFQAS SLGQACDIID EMRAWRGKHI DELDEYSRKG SFDENGFQKS
110 120 130 140 150
TIFLGYTSNL ISSGLRETLR YLVQHNMVSA IVTTAGGVEE DIIKCLAPTY
160 170 180 190 200
LGEFTLKGAA LRDKGMNRIG NLLVPNNNYC KFEEWIVPIL DAMLAEQEAY
210 220 230 240 250
VAQQGKDCLG VNTDVDSPIW TPSKLCDRLG KEINDESSVL YWAHKNKIPV
260 270 280 290 300
FCPAITDGSI GDMLFLHTFR ASPQQLRLDL VADIRKINSM SMEASQAGMI
310 320 330 340 350
ILGGGLIKHH IANACLMRNG ADYAVYINTG QEFDGSDAGA RPDEAVSWGK
360 370 380
IKVEAKSVKV YADVTTVFPL IVAATFANGK KN
Length:382
Mass (Da):41,984
Last modified:July 5, 2004 - v1
Checksum:iB674D54C4066F144
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016814 Genomic DNA. Translation: AAS50330.1.
RefSeqiNP_982506.1. NM_207859.1.

Genome annotation databases

EnsemblFungiiAAS50330; AAS50330; AGOS_AAL036W.
GeneIDi4618497.
KEGGiago:AGOS_AAL036W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016814 Genomic DNA. Translation: AAS50330.1.
RefSeqiNP_982506.1. NM_207859.1.

3D structure databases

ProteinModelPortaliQ75EW4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS50330; AAS50330; AGOS_AAL036W.
GeneIDi4618497.
KEGGiago:AGOS_AAL036W.

Phylogenomic databases

HOGENOMiHOG000228838.
InParanoidiQ75EW4.
KOiK00809.
OMAiHDFTISA.
OrthoDBiEOG092C3JEN.

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_ASHGO
AccessioniPrimary (citable) accession number: Q75EW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: October 5, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.