Reviewed, UniProtKB/Swiss-Prot Q75EN3 (ATG15_ASHGO)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
Ordered Locus Names:	AAR046C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Putative lipase ATG15		PRO_0000090363
Regions
	Topological domain	1 – 45	45	Cytoplasmic Potential
	Transmembrane	46 – 66	21	Signal-anchor for type II membrane protein Potential
	Topological domain	67 – 544	478	Lumenal Potential
Sites
	Active site	362	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75EN3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2894640F05A60631	FASTA	544	60,525
	10 20 30 40 50 60 MVADFDSGWY EGAGDELGQG ARNGVLRERL GGNEKAQVVR SRRKAVAWNV LMVLGLILYV 70 80 90 100 110 120 LYSACFAQAR QWWRTNGMAR GSQAGRFQLV QVHHHGVGAE HATHLVLDVD AEFRKEAAAE 130 140 150 160 170 180 YSVFALHGEG QELWPEAGRR RAANPFEYAY ALREERATVV RAQQQVPEFM EPYLDFALER 190 200 210 220 230 240 PDLAEQIKMD WGYENMFVPN ITDKETVISL ALMSSNAYVR LPNTGNWRNV TSWNSSVEGD 250 260 270 280 290 300 QIHLGWDENG LRAHVFANED RSVVVLALKG TSSQVLPGSG DDDETSANDK LNDNLLFSCC 310 320 330 340 350 360 CARVSYLWTT VCDCYIKSYT CEESCLESEL RRKDRYYQAG LDMYKQVLAE FPDASIWLTG 370 380 390 400 410 420 HSLGGALASL VARTYGVPAV TFEAPGELLA TQRLHLPQPP GLPNQLDTVW HFGHTADPIF 430 440 450 460 470 480 MGTCNGASSA CSIAGYAMET SCHSGKSCVY DVVNDRGWRV NLLHHRIHTV IDKILDDYDA 490 500 510 520 530 540 VAACVPPDIC HDCYNWNYVR WRDGTTSPMP SSVASKPTPT PTSPGSPSST CKGRNWFGYC TEYA

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016814 Genomic DNA. Translation: AAS50411.1.
RefSeq	NP_982587.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75EN3.
Genome annotation databases
GeneID	4618494.
GenomeReviews	Gene locus AAR046C in contig AE016814_GR.
KEGG	ago:AGOS_AAR046C.
NMPDR	fig|33169.1.peg.232.
Organism-specific databases
AGD	AAR046C.
Phylogenomic databases
HOGENOM	Q75EN3.
OMA	RTIELEW.
Enzyme and pathway databases
BRENDA	3.1.1.3. 279361.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_ASHGO

Accession

Primary (citable) accession number: Q75EN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)