Reviewed, UniProtKB/Swiss-Prot Q75EK2 (NMT_ASHGO)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Name:	NMT1
Ordered Locus Names:	AAR077C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064234
Regions
	Region	38 – 41	4	Myristoyl CoA-binding By similarity
	Region	168 – 204	37	Myristoyl CoA-binding By similarity
Sites
	Active site	452	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75EK2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2B11E19DFA50097F	FASTA	452	52,754
	10 20 30 40 50 60 MGDSGDAQKM QRLLEMLALN SGDMSKLTQQ QRKAFEEYKF WKTQPVARFD EKVEEEGPIN 70 80 90 100 110 120 PPRRVEDVRD EPYPLLEEFE WRTMDITTGQ DLEDVFVLLN ENYIEDKDST FRFNYTREFF 130 140 150 160 170 180 NWALKPPGWR KEWHVGVRVR QSGRLVAFIS AVPTTLEVRG REMKSVEINF LCIHKKLRSK 190 200 210 220 230 240 RLAPILIKEI TRRVNKCDIW HALYSAGIVL PSPISTCRYT HRPLNWSKLF DVGFTALPAN 250 260 270 280 290 300 ATKTQMLAKY TLPKKPLVEG LRPMTDADVD GAFDLFNRYQ KRFELIQTFD KSEFRHWFLG 310 320 330 340 350 360 NEETPSVIYS YVVQNSEGKI TDFVSFYSLP FTILKNPLHK ELGIGYLFYY ASDADFDYED 370 380 390 400 410 420 RYDPTATELL RKRLTQLIND VCILARDLKM DVFNALTSQD NALFLEDLKF GPGDGFLNFY 430 440 450 LFNYRANPIR GGLTEDKKFD AKNRSNQGVV ML

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016814 Genomic DNA. Translation: AAS50442.1.
RefSeq	NP_982618.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75EK2.
Genome annotation databases
GeneID	4618539.
GenomeReviews	Gene locus AAR077C in contig AE016814_GR.
KEGG	ago:AGOS_AAR077C.
NMPDR	fig|33169.1.peg.263.
Organism-specific databases
AGD	AAR077C.
Phylogenomic databases
HOGENOM	Q75EK2.
OMA	VEDDDSM.
Enzyme and pathway databases
BRENDA	2.3.1.97. 279361.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_ASHGO

Accession

Primary (citable) accession number: Q75EK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)