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Protein

5-aminolevulinate synthase, mitochondrial

Gene

HEM1

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.By similarity

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, mitochondrial (HEM1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei218 – 2181SubstrateBy similarity
Binding sitei237 – 2371SubstrateBy similarity
Binding sitei270 – 2701Pyridoxal phosphateBy similarity
Binding sitei298 – 2981Pyridoxal phosphateBy similarity
Binding sitei342 – 3421Pyridoxal phosphateBy similarity
Active sitei345 – 3451By similarity
Binding sitei374 – 3741Pyridoxal phosphate; shared with dimeric partnerBy similarity
Binding sitei375 – 3751Pyridoxal phosphate; shared with dimeric partnerBy similarity
Binding sitei460 – 4601SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:HEM1
Ordered Locus Names:ABL104C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome II

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646MitochondrionSequence analysisAdd
BLAST
Chaini47 – 5565105-aminolevulinate synthase, mitochondrialPRO_0000001236Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei345 – 3451N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ75DX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000221020.
InParanoidiQ75DX7.
KOiK00643.
OMAiKLAQYPK.
OrthoDBiEOG092C1OK6.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q75DX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLARQSAK ICPFVSRVTS SMQQVQVLHK TNMSAMAQQC PVMRRAMAAR
60 70 80 90 100
GYVTASPPAG AAAADVGEAR PITPVLERGT QERTFDYDGL FETELQKKRL
110 120 130 140 150
DSSYRFFNNI NRLAKEYPMA HRLEEEDKVT VWCSNDYLTY SRNEKVMETM
160 170 180 190 200
KRTIDKYGAG AGGTRNIAGH NRHAMRLEAE LAALHKKEGA LVFSSCFVAN
210 220 230 240 250
DAVLSLLGQK MPNMVIFSDE MNHASMIMGI KHANVQKHIF RHNDLQHLEE
260 270 280 290 300
LLAMYPKSQP KLIAFESVYS MSGSVADIRK ICDLAEKYGA LTFLDEVHSV
310 320 330 340 350
GLYGPHGAGV AEHLDFEAHR KAGLASPAQT TVLDRVDMIT ATLGKSFGSV
360 370 380 390 400
GGYLAASEKL VDFVRSYAPG FIFTSSLPPA VMAGSAAAVF DQRSSLHLRQ
410 420 430 440 450
LQQKHTSYVK TGLGDLGIPV QPNPSHIVPV LVGNPDLAKR ASDILMEKHR
460 470 480 490 500
IYVQAINFPT VPRGTERLRI TPTPGHTNDL SDVLLDAMDD VWKTLQLPRV
510 520 530 540 550
SDWAAHGGLL GVGEPDYVPE ANLWTEEQMS LTNDDLHPSV FSPVEKFLEV

SSGIKA
Length:556
Mass (Da):61,222
Last modified:July 5, 2004 - v1
Checksum:iDABDD7E6FEB4E764
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016815 Genomic DNA. Translation: AAS50667.1.
RefSeqiNP_982843.1. NM_208196.1.

Genome annotation databases

EnsemblFungiiAAS50667; AAS50667; AGOS_ABL104C.
GeneIDi4618923.
KEGGiago:AGOS_ABL104C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016815 Genomic DNA. Translation: AAS50667.1.
RefSeqiNP_982843.1. NM_208196.1.

3D structure databases

ProteinModelPortaliQ75DX7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS50667; AAS50667; AGOS_ABL104C.
GeneIDi4618923.
KEGGiago:AGOS_ABL104C.

Phylogenomic databases

HOGENOMiHOG000221020.
InParanoidiQ75DX7.
KOiK00643.
OMAiKLAQYPK.
OrthoDBiEOG092C1OK6.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM1_ASHGO
AccessioniPrimary (citable) accession number: Q75DX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.