Reviewed, UniProtKB/Swiss-Prot Q75DS1 (RPA2_ASHGO)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I polypeptide 2
      Short name=RNA polymerase I subunit 2

Gene names

Name:	RPA2
Ordered Locus Names:	ABR029W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	1198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1198	1198	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048077
Regions
	Zinc finger	1099 – 1126	28	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75DS1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C2B1FEBC065347BD	FASTA	1,198	134,448
	10 20 30 40 50 60 MSVMEPLSAN RTAQFRTLER EARFVSPPKD KSAYPLLYEA VEPHVGSFNA LTEGPGGGLL 70 80 90 100 110 120 NLGARDIGAK VVFDGKASDE NPNYLGNKLA LSVTQVSLTK PMSNDGVTAA AERNVFPAEA 130 140 150 160 170 180 RKRLTTYRGK LLLKLNWSVN DGEETFSEVR DCGPLPVMLQ SNRCHLHKMS PQELVEHKEE 190 200 210 220 230 240 SDELGGYFIV NGIEKLIRML IVQRRNHPMA IIRPSFANRG TSYSHYGVQI RCVRPDQTSQ 250 260 270 280 290 300 TNVLHYLNDG QVTFRFSWRK NEYLVPVVLI LKALTDASDR EIFDGIVGAD TSNSFLTDRL 310 320 330 340 350 360 ELLLRGFKKR FPQLLNRRQV LQYLGDKFRV VLQASPDMSD YLVGQELLRR IVLVHLGDEN 370 380 390 400 410 420 TDKSNMLMFM IRKLYSLVAG ECCPDNPDAT QHQEVLLGGF LYGMIVKEKI EEYLQNIKLQ 430 440 450 460 470 480 IQADVNRGMP VDFKDRKYMT RVLTRINENI GSKLQYFLST GNLVSQSGLD LQQVSGYTVV 490 500 510 520 530 540 AEKINFYRFI SHFRMVHRGS FFAQLKTTTV RKLLPESWGF LCPVHTPDGS PCGLLNHFAH 550 560 570 580 590 600 KCKISTTQSD VSKIPTLLYS LGVSPAAHVT AAGPSLCCVQ LDGKIVGWCS HEQGRIVADT 610 620 630 640 650 660 LRYWKVEGKT DGLPLDLEIG YVPASKRGQY PGLYLFGGHS RMMRPVRYLP LDKQDIVGPF 670 680 690 700 710 720 EQVYMDIAVT PEENPLITSI LMLNFHQQII LSILANLTPF LLTLTNLPKI CTNYQMGKQT 730 740 750 760 770 780 MGTPGVALCH RSDNKLYRLQ SGQTPIVKAN LYDDYGMDNF PNGTNAVVAV ISYTGYDMDD 790 800 810 820 830 840 AMIINKSADE RGFSYGTVYK TEKIDLSLSR GRGDPVTQHF GFGTDEWPKE WLEKLDEDGL 850 860 870 880 890 900 PIIGSYVEEG DPICAYFDDT LNKTKIKTYH SSEPAYIEEV TLIGDESNKF QELQYITIKY 910 920 930 940 950 960 RIRRVPQIGD KFSSRHGQKG VCSRKWPTVD MPFSETGIQP DVIINPHAFP SRMTIGMFVE 970 980 990 1000 1010 1020 SLAGKAGALH GIAQDATPWT FSEEDTPADY FGDQLLKAGY NYHGNEPMYS GATGEELRAD 1030 1040 1050 1060 1070 1080 IYIGVVYYQR LRHMVNDKFQ VRSTGPVNSL TMQPVKGRKR HGGIRVGEME RDALIGHGTS 1090 1100 1110 1120 1130 1140 FLLQDRLLNS SDYTQSAVCR ECGSILTTQS SVPKIGSMVT IRCRRCAISF DEAKKIITQQ 1150 1160 1170 1180 1190 DSEDSIFIDD SHIWEDGQGN KFVGGGNTTT VAIPFVLKYL DSELAAMGIR LRYNVDPK

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016815 Genomic DNA. Translation: AAS50799.1.
RefSeq	NP_982975.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75DS1.
Genome annotation databases
GeneID	4618998.
GenomeReviews	Gene locus ABR029W in contig AE016815_GR.
KEGG	ago:AGOS_ABR029W.
NMPDR	fig|33169.1.peg.620.
Organism-specific databases
AGD	ABR029W.
Phylogenomic databases
HOGENOM	Q75DS1.
OMA	CGLMNHM.
Enzyme and pathway databases
BRENDA	2.7.7.6. 279361.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA2_ASHGO

Accession

Primary (citable) accession number: Q75DS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)