Q75D46 (BUR1_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase BUR1
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	BUR1
Ordered Locus Names:	ABR177C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 753	753	Serine/threonine-protein kinase BUR1		PRO_0000085677
Regions
	Domain	56 – 372	317	Protein kinase
	Nucleotide binding	62 – 70	9	ATP By similarity
	Compositional bias	420 – 483	64	Gln-rich
	Compositional bias	528 – 579	52	Tyr-rich
Sites
	Active site	201	1	Proton acceptor By similarity
	Binding site	85	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75D46 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8F60C0B2E803FFF0	FASTA	753	84,823
	10 20 30 40 50 60 MSQEGSAKRA DFKYKIGKIK HTPPVLKDVR SGLEFIELEK RDDEKVYGVT KFLGNYKEEK 70 80 90 100 110 120 KLGQGTFGEV YRGVHLATQR QVAMKKILVK TENDLFPITA QREITILKRL NHRNVVQLIE 130 140 150 160 170 180 MVYDYPPAQN GAAYGQDSSQ ASASADTMKS FYMILPYMVA DLSGILHNPR VTLEMADIKN 190 200 210 220 230 240 MMLQILEGIN YIHCKKFMHR DIKTANILLD HKGILKIADF GLARNYYGAP PNLKYPGGAG 250 260 270 280 290 300 TDAKYTSVVV TRWYRAPELV LGDKNYTTAV DIWGIGCVFA EFFEKRPILQ GKTDIDQGHV 310 320 330 340 350 360 IFKLMGTPSD SDWQLARYLP GAELTRTSYE PTYKERFGKY LTEKGLDLLS TLLSLDPYKR 370 380 390 400 410 420 LTAMAAMQHP FFSEDPLPKL QLTLPCEESH ETDIARYGQE MQKTVSNLPP AAPSGHAVEQ 430 440 450 460 470 480 SQTQQQSSRH HQSQPYQSLP PQKSSQPHPQ QIPEAAPVQQ PSQQPPQEPS AHQQQYSSRG 490 500 510 520 530 540 PQQHRTTPAP TSLPPKPKPA PPGARYNREA PPQNKPVKPL TRHLGAQYQH NNYGNYYNHW 550 560 570 580 590 600 QGGERYYETY NDGYPHADRY SDDTHYNGYG YRSRGYQRYD MRREGESRYN QRPYAPQQGA 610 620 630 640 650 660 AYRPNRRDTY KLVDRTGQPY PPERVPNPEL HPSPAPRPKR TLTNTTQLSS HSSAATGSTA 670 680 690 700 710 720 ASGSAVSNSS EPVRRSSTAS LDNSESGNIL QQKPPPPVAN PLEARIYGRD EVQSNHPGGR 730 740 750 LYPRSTPDSK NSEGAAPSEA STDPTKRNIV DYY

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016815 Genomic DNA. Translation: AAS50949.1.
RefSeq	NP_983125.1. NM_208478.1.
3D structure databases
ProteinModelPortal	Q75D46.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ABR177C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS50949; AAS50949; AGOS_ABR177C.
GeneID	4619235.
KEGG	ago:AGOS_ABR177C.
Phylogenomic databases
eggNOG	COG0515.
KO	K15562.
OMA	CEESHET.
OrthoDB	EOG41K2MW.
PhylomeDB	Q75D46.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_ASHGO

Accession

Primary (citable) accession number: Q75D46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program