Reviewed, UniProtKB/Swiss-Prot Q75CQ8 (FRE8_ASHGO)

Names and origin

Protein names

Recommended name:
    Probable ferric reductase transmembrane component
    EC=1.16.1.7
Alternative name(s):
    Ferric-chelate reductase 8

Gene names

Name:	FRE8
Ordered Locus Names:	ACL139W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required for the uptake of Fe3+ ions. May participate in the transport of electrons from cytoplasm to an extracellular substrate (Fe3+ ion) via FAD and heme intermediates. Involved in iron homeostasis By similarity.
Catalytic activity	2 Fe2+ + NAD+ = 2 Fe3+ + NADH.
Cofactor	FAD Probable.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 ferric oxidoreductase domain.

Ontologies

Keywords
Biological process	Electron transport Ion transport Iron transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	FAD Iron NAD
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferric-chelate reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Probable ferric reductase transmembrane component		PRO_0000239646
Regions
	Transmembrane	23 – 43	21	Potential
	Transmembrane	79 – 99	21	Potential
	Transmembrane	111 – 131	21	Potential
	Transmembrane	147 – 167	21	Potential
	Transmembrane	178 – 198	21	Potential
	Transmembrane	205 – 225	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	392 – 412	21	Potential
	Domain	108 – 666	559	Ferric oxidoreductase
	Nucleotide binding	308 – 314	7	FAD Potential
	Nucleotide binding	431 – 439	9	NAD Potential
Amino acid modifications
	Glycosylation	506	1	N-linked (GlcNAc...) Potential
	Glycosylation	644	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75CQ8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0700CDD72C5E2E91	FASTA	686	77,515
	10 20 30 40 50 60 MGGAWQEAVV RSIGAADERM VRLSGWASLA LALGLVCVVV PVVNYMRLNP WVFRVMHHWR 70 80 90 100 110 120 HHVMRRHGAM CRKLVQQRTL WLALLWCMLG GACAVVGSAD VVVVTKRLGR VAAAFMPALF 130 140 150 160 170 180 LLTLRPSPLP YTLYLSLLPM HKWLGRVVVL QATVHSALYT WYFATSGKMA KMKKTANWMG 190 200 210 220 230 240 AVALLAFVLI AATSLPAVRR RRFRTFYYVH YVGTWVSVLA VHVHSRPPVT TYTVLNVALL 250 260 270 280 290 300 LYQAWYRISR MSTTTVTVVP ISTSLALLEF PLADLVEKPQ LPSGHVRINL RAPSILGRVF 310 320 330 340 350 360 QHIMPMQHPF TVASLPTDTT VRLIVRKSRF PLVNNGKYYV TGAFEPKVDF LSHRKRRMPG 370 380 390 400 410 420 SWAPEPASPF QCQSPSLQSS PLHYNIKASR VLMVVGGSAI SFGLPFLRIL NFNGVNVRLI 430 440 450 460 470 480 WVCRDYHDLR ILSQFRSNFN GLEIYVTGTN CEEQDLNIDY VDYDERSSEG ERDPERCALL 490 500 510 520 530 540 SPKASEYNCL TPTTGGSTLS GRDAHNYSTF QNRCHSCKHL DRGCRDLEDH AIADINDEID 550 560 570 580 590 600 FTDFFSTRHS TSKYHPKENS KLPVITKDSV FRKPNYVVPP VFDRYDLKTG FTTYTTREKL 610 620 630 640 650 660 SIPTGVKLFF GRPVLSPRDY HWCLQKECIG PSETNECCRA DIANSTHVDD LARVWVLAAG 670 680 PQALVEATRI WASDGGLHFH EESFKV

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016816 Genomic DNA. Translation: AAS51089.1.
RefSeq	NP_983265.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4619385.
KEGG	ago:AGOS_ACL139W.
NMPDR	fig|33169.1.peg.910.
Organism-specific databases
AGD	ACL139W.
Phylogenomic databases
HOGENOM	Q75CQ8.
OMA	Q75CQ8. FHEEAFY.
Enzyme and pathway databases
BRENDA	1.16.1.7. 279361.
Family and domain databases
InterPro	IPR013130. Fe3_reduct_TM_N. [Graphical view]
Pfam	PF01794. Ferric_reduct. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRE8_ASHGO

Accession

Primary (citable) accession number: Q75CQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)