Reviewed, UniProtKB/Swiss-Prot Q75CM8 (TRXB_ASHGO)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
    EC=1.8.1.9

Gene names

Name:	TRR1
Ordered Locus Names:	ACL109C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Thioredoxin reductase		PRO_0000166761
Regions
	Nucleotide binding	33 – 45	13	FAD By similarity
	Nucleotide binding	288 – 297	10	FAD By similarity
Amino acid modifications
	Disulfide bond	142 ↔ 145		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75CM8-1 [UniParc]. Last modified November 9, 2004. Version 2. Checksum: F7151B685B7802FC	FASTA	319	34,117
	10 20 30 40 50 60 MVHHKVTIIG SGPAAHTAAI YLARAEIKPT LYEGMFANGV AAGGQLTTTT EIENFPGFPD 70 80 90 100 110 120 GLTGSDLMER MKAQSVKFGT EVVTETVAKV DLSARPFKLW TEFNEDEEPT TTDAIILATG 130 140 150 160 170 180 ASAKRLGLPG EETYWQRGIS ACAVCDGAVP IFRNKPLAVV GGGDSACEEA SFLTKYGSKV 190 200 210 220 230 240 FMLVRKDHMR ASTIMQRRVE RNEKIEVLYN TAPVEAKGDG SLLDALRVRD TRTGEESDLP 250 260 270 280 290 300 VNGLFYAIGH TPATQLVAGQ VDLDESGYVK TVPGSTLTNV PGLFAAGDVQ DSRYRQAVTS 310 AGSGCMAALD AEKFLSELE

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016816 Genomic DNA. Translation: AAS51119.1. Different initiation.
RefSeq	NP_983295.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4619415.
KEGG	ago:AGOS_ACL109C.
NMPDR	fig|33169.1.peg.940.
Organism-specific databases
AGD	ACL109C.
Phylogenomic databases
HOGENOM	Q75CM8.
Enzyme and pathway databases
BRENDA	1.8.1.9. 279361.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_ASHGO

Accession

Primary (citable) accession number: Q75CM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	November 9, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)