Reviewed, UniProtKB/Swiss-Prot Q75CE1 (PYRD_ASHGO)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase, mitochondrial
      Short name=DHOdehase
      Short name=DHODase
      Short name=DHOD
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase

Gene names

Name:	URA9
Ordered Locus Names:	ACL035C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FAD per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subcellular location	Mitochondrion inner membraneBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 444		Dihydroorotate dehydrogenase, mitochondrial		PRO_0000029890
Sites
	Active site	253	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75CE1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 27E890B278E04733	FASTA	444	48,288
	10 20 30 40 50 60 MSYKMFGRCR TACFRSPNLF AANKGMPLQA SVAGGASRYI IGTASVLVGA MAGFYIMNSR 70 80 90 100 110 120 SAIHEYVSCP LVRLVTPDAE DGHKLGIWFL KYGLAPKLMF DKDDPVLKVE VFGKTMSNPI 130 140 150 160 170 180 GCAAGLDKDG EAIDGIAQSG FGYVEIGTVT PLAQPGNPKP RFFRLPMDEA VINRYGFNSS 190 200 210 220 230 240 GHKKVYDNVL SRVRQFLGAY FRDDTVNSLA LYKDKLLGIN LGKNKNGDEV EDYLRGVEKF 250 260 270 280 290 300 QSLADVLVIN VSSPNTPGLR DLQQEGRLTE LLSMIVAKRN SLINEGNVLG ASTHKPPVLV 310 320 330 340 350 360 KIAPDLTEPE LQSIAEAAKK SSIDGIIVSN TTIQRPDSLY TVDEELKNQA GGLSGKPVKP 370 380 390 400 410 420 FALKALRTIH QYTKDTNLVL VGCGGISSGQ DAIEFAKAGA HFVQLYTAYA YRGPGLIARI 430 440 KDEVTEELRN EGKTWMEIIG QDAK

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016816 Genomic DNA. Translation: AAS51193.1.
RefSeq	NP_983369.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4619494.
KEGG	ago:AGOS_ACL035C.
NMPDR	fig|33169.1.peg.1014.
Organism-specific databases
AGD	ACL035C.
Phylogenomic databases
HOGENOM	Q75CE1.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

PYRD_ASHGO

Accession

Primary (citable) accession number: Q75CE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)