Reviewed,
UniProtKB/Swiss-Prot Q75CA4 (DNLI4_ASHGO)
Last modified
February 9, 2010.
Version 47.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA ligase 4 EC=6.5.1.1 Alternative name(s): DNA ligase IV Polydeoxyribonucleotide synthase [ATP] 4 | ||||
| Gene names |
| ||||
| Organism | Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome] | ||||
| Taxonomic identifier | 33169 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium |
Protein attributes
| Sequence length | 981 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity. |
| Catalytic activity | ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). |
| Cofactor | Magnesium By similarity. |
| Subcellular location | Nucleus By similarity. |
| Sequence similarities | Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA recombination DNA repair DNA replication |
| Cellular component | Nucleus |
| Domain | Repeat |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repairInferred from electronic annotation. Source: UniProtKB-KW DNA replicationInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA bindingInferred from electronic annotation. Source: InterPro DNA ligase (ATP) activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 981 | 981 | DNA ligase 4 | PRO_0000278374 | |||||
Regions | |||||||||
| Domain | 721 – 819 | 99 | BRCT 1 | ||||||
| Domain | 875 – 980 | 106 | BRCT 2 | ||||||
Sites | |||||||||
| Active site | 322 | 1 | N6-AMP-lysine intermediate By similarity | ||||||
| Metal binding | 380 | 1 | Magnesium 1 Potential | ||||||
| Metal binding | 484 | 1 | Magnesium 2 Potential | ||||||
| Binding site | 320 | 1 | ATP By similarity | ||||||
| Binding site | 327 | 1 | ATP By similarity | ||||||
| Binding site | 344 | 1 | ATP By similarity | ||||||
| Binding site | 489 | 1 | ATP By similarity | ||||||
| Binding site | 500 | 1 | ATP By similarity | ||||||
| Binding site | 506 | 1 | ATP By similarity | ||||||
Sequences
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References
| [1] | "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE016816 Genomic DNA. Translation: AAS51235.1. |
| RefSeq | NP_983411.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1Z56 based on UniProtKB Q08387. |
| SMR | Q75CA4. Positions 724-978. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q75CA4. |
Genome annotation databases | |
| GeneID | 4619536. |
| GenomeReviews | Gene locus ACR008W in contig AE016816_GR. |
| KEGG | ago:AGOS_ACR008W. |
| NMPDR | fig|33169.1.peg.1056. |
Organism-specific databases | |
| AGD | ACR008W. |
Phylogenomic databases | |
| eggNOG | fuNOG06512. |
| HOGENOM | HBG396164. |
| OMA | CSIANGI. |
| OrthoDB | EOG922C2R. |
Enzyme and pathway databases | |
| BRENDA | 6.5.1.1. 279361. |
Family and domain databases | |
| InterPro | IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view] |
| Gene3D | G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. |
| Pfam | PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view] |
| SMART | SM00292. BRCT. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR00574. dnl1. 1 hit. |
| PROSITE | PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DNLI4_ASHGO | ||||||||
| Accession | Primary (citable) accession number: Q75CA4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||

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