Q75CA4 (DNLI4_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase 4
EC=6.5.1.1

Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	ACR008W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	981 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA ligase IV complex Inferred from sequence or structural similarity. Source: RefGenome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from sequence or structural similarity. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 981	981	DNA ligase 4		PRO_0000278374
Regions
	Domain	721 – 819	99	BRCT 1
	Domain	875 – 980	106	BRCT 2
Sites
	Active site	322	1	N6-AMP-lysine intermediate By similarity
	Metal binding	380	1	Magnesium 1 Potential
	Metal binding	484	1	Magnesium 2 Potential
	Binding site	320	1	ATP By similarity
	Binding site	327	1	ATP By similarity
	Binding site	344	1	ATP By similarity
	Binding site	489	1	ATP By similarity
	Binding site	500	1	ATP By similarity
	Binding site	506	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75CA4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9883B3EEDACA8A25	FASTA	981	111,881
	10 20 30 40 50 60 MVIYPWLKNT TELTIALPKK PIIHANMDVL GSPRGGTPTG EHEINAQDGS PINFSPSPDF 70 80 90 100 110 120 CWLCDELFIK LEEVALKKKD LGKPRKVRNL EITSNFVSLW RKTVGNDIYP ALVLSLPYND 130 140 150 160 170 180 RRSYRVKDVT LVKALCKHMK LPRNSETERR LLHWKQNAPR GVKLSTFCVE ELQKRRREPV 190 200 210 220 230 240 VPKRMSIDEV NGMLDKLEHE SNVGKWSYIS LAESPAFNYC LEHMSYVELR FFFDIVLKVP 250 260 270 280 290 300 IVSGLESLLL SCWHPDAESY FKVVSDLRIV AHTLYDPNER LEKNDLSVRI GYAFAPHMAQ 310 320 330 340 350 360 RVKIPYEKVS TKLGNDFYVE EKMDGDRIQV HYMDYGNSIA YFSRNGINYT YLYGENSSKG 370 380 390 400 410 420 SISNHLKFVE GVKECILDGE MVSYDKEMQC ILPFGLTKSG ASHQVNFETT GHTEPTYRPL 430 440 450 460 470 480 YAVFDLLYLN GQLLTNQDVV KRKEYLEKIL IPSKNVVHLL SGPRCSDAEA ITAALGAAVA 490 500 510 520 530 540 HGSEGIVLKK ARSKYSVGKR DDSWIKIKPE YLENFGENMD LVVIGRDKGR KDSFICALAV 550 560 570 580 590 600 TDDSEKNNPS SYESGSDSDS DSEPIIVQPK IEKFISFCSI ANGISNEEFK EIDRLTRGNW 610 620 630 640 650 660 FPYDERKPPT DWVEFGTKTP REWIDPKNSV VLEVKARSID NEESKSDLYK TGSTLYNAYC 670 680 690 700 710 720 KRIRHDKNWS TASTVAEYDT AREARSYFNV SQNAKFGKDR SSPRKRRTFH LVGDIDVTKP 730 740 750 760 770 780 SKADFLKGYY FYVTSGYFDL QSKKNIDASE IGEAVVSCGG TYIHNLRIRA SLDKLYILGC 790 800 810 820 830 840 KDTRELKMLI ERGYDIIHPE WLMDCVKYGT MLQIEPKYVY SASEELMKQA RNQEDKYGES 850 860 870 880 890 900 YQLPVTEDTL KALANKQVEE GYASEMGTDA VSEYERLLIF KGWLFYILDD YAYHSSWSDI 910 920 930 940 950 960 VKWNIESCGG EVTNDLELAT IVVAVKDCFS QLSLQAVRNN IGARITGSND VQPIPKIVTS 970 980 EWVEACMEAQ YLVDEDEYAA I

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016816 Genomic DNA. Translation: AAS51235.1.
RefSeq	NP_983411.1. NM_208764.2.
3D structure databases
HSSP	HSSP built from PDB template 1Z56 based on UniProtKB Q08387.
ProteinModelPortal	Q75CA4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75CA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4619536.
GenomeReviews	Gene locus ACR008W in contig AE016816_GR.
KEGG	ago:AGOS_ACR008W.
NMPDR	fig|33169.1.peg.1056.
Organism-specific databases
AGD	ACR008W.
Phylogenomic databases
eggNOG	fuNOG06512.
HOGENOM	HBG396164.
OMA	CSIANGI.
OrthoDB	EOG41K2MD.
PhylomeDB	Q75CA4.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10777.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF52113. BRCT. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_ASHGO

Accession

Primary (citable) accession number: Q75CA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program