Q75C78 (GRP78_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
78 kDa glucose-regulated protein homolog
Short name=GRP-78

Alternative name(s):
Immunoglobulin heavy chain-binding protein homolog
Short name=BIP

Gene names

Name:	KAR2
Ordered Locus Names:	ACR038W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC ER-associated protein catabolic process Inferred from electronic annotation. Source: EnsemblFungi SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from electronic annotation. Source: EnsemblFungi karyogamy involved in conjugation with cellular fusion Inferred from electronic annotation. Source: EnsemblFungi posttranslational protein targeting to membrane, translocation Inferred from electronic annotation. Source: EnsemblFungi response to unfolded protein Inferred from electronic annotation. Source: EnsemblFungi
Cellular_component	luminal surveillance complex Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: EnsemblFungi
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 674	640	78 kDa glucose-regulated protein homolog		PRO_0000013576
Regions
	Motif	671 – 674	4	Prevents secretion from ER By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75C78 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EA3DCF15A5A48547	FASTA	674	73,898
	10 20 30 40 50 60 MAFGKVSNWA VPLTALMYAM ALVCLPMFAG GSRGLVYAAD TDAENYGTVI GIDLGTTYSC 70 80 90 100 110 120 VALMRNGKTE ILANEQGNRI TPSYVAFTDD ERLIGDAAKN QVANNPKNTI FDIKRLIGLK 130 140 150 160 170 180 YNDKTVQREI KHLPFEVVDK NGMPAVAVTV KGERKLFTPE EISGMVLGKM KQIAEEYLGK 190 200 210 220 230 240 KVTHAVVTVP AYFNDAQRQA TKDAGAIAGL NILRIVNEPT AAAIAYGLDK TEDEHRIVVY 250 260 270 280 290 300 DLGGGTFDVS LLSIENGVFE VQATAGDTHL GGEDFDYKLV RHFLKVFKKK HGVDVSSNAK 310 320 330 340 350 360 AMAKLKREAE KAKRALSSQM STRVEIDSFV DGIDFSETLT RAKFEEMNLD LFKRTLKPVE 370 380 390 400 410 420 KVLQDAGLKK EDIDDIVLVG GSTRIPKVQE LLENFFNKKA SKGINPDEAV AYGAAVQAGV 430 440 450 460 470 480 LSGEEGVEDI VLLDVNPLTL GIEVTGGIMT PLIKRNTPIP TKKSQIFSTA VDNQKTVMIQ 490 500 510 520 530 540 VYEGERAMAK DNNHLGKFEL SGIPPAPRGI PQIEVTFALD ANGILKVSAT DKGTGKSESV 550 560 570 580 590 600 TITNEKGRLS KDDIERMVAE AENYQKEDEE IRAKVEARHK LENYAHSLKN QVNGDLGDKL 610 620 630 640 650 660 EEDDKETLLE AANDVLEWLE DNSDSATKEE FNEKFESLSQ TAYPITSKLY GAGASDATDD 670 EDDESDDYFD HDEL

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016816 Genomic DNA. Translation: AAS51265.1.
RefSeq	NP_983441.1. NM_208794.1.
3D structure databases
ProteinModelPortal	Q75C78.
SMR	Q75C78. Positions 47-585.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ACR038W.
Proteomic databases
PRIDE	Q75C78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS51265; AAS51265; AGOS_ACR038W.
GeneID	4619566.
KEGG	ago:AGOS_ACR038W.
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HOG000228135.
KO	K09490.
OMA	LINRNTA.
OrthoDB	EOG4X0R1M.
PhylomeDB	Q75C78.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRP78_ASHGO

Accession

Primary (citable) accession number: Q75C78

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program