Reviewed, UniProtKB/Swiss-Prot Q75C62 (MCR1_ASHGO)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
Ordered Locus Names:	ACR054C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	NADH-cytochrome b5 reductase 2		PRO_0000330169
Regions
	Transmembrane	12 – 29	18	Potential
	Domain	57 – 161	105	FAD-binding FR-type
	Nucleotide binding	164 – 199	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75C62-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AB7BECE740609D43	FASTA	306	33,771
	10 20 30 40 50 60 MLSRITRSSK ALNFALGTTA VAAAGYLYAQ SRGNLSTSLN KGDVTGQAAA QTFTGDGQWH 70 80 90 100 110 120 ALKIAKIEQV SHDTRRFTFA LPSQDHVTGL TTASALLAKY VTPKGSNVIR PYTPVSDNMA 130 140 150 160 170 180 RGMFQLVIKH YDGGKFTTHL FGLKENDTVE FKGPIQKWRW DPNMFDSIVL MGAGTGITPL 190 200 210 220 230 240 FQMMHHIAEN PTDNTKVHLL YGNKTPQDIL LRKELEELAS KYPDQVKVTY FVDKPEGDYK 250 260 270 280 290 300 GEKGFITKDF LKQNLPTPGS NSHIFVCGPP PFMDAFSGNK VSPSDQGQVT GVLSELGYTK DHVYKF

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016816 Genomic DNA. Translation: AAS51281.1.
RefSeq	NP_983457.1.
3D structure databases
HSSP	HSSP built from PDB template 1QX4 based on UniProtKB P20070.
SMR	Q75C62. Positions 56-306.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75C62.
Genome annotation databases
GeneID	4619582.
GenomeReviews	Gene locus ACR054C in contig AE016816_GR.
KEGG	ago:AGOS_ACR054C.
NMPDR	fig|33169.1.peg.1102.
Organism-specific databases
AGD	ACR054C.
Phylogenomic databases
eggNOG	fuNOG07850.
HOGENOM	HBG591994.
OMA	PVASCIT.
OrthoDB	EOG9TB5TC.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_ASHGO

Accession

Primary (citable) accession number: Q75C62

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)