Reviewed, UniProtKB/Swiss-Prot Q75BY0 (DGA1_ASHGO)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20

Gene names

Name:	DGA1
Ordered Locus Names:	ACR140C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. May be involved in lipid particle synthesis from the endoplasmic reticulum and ergosterol biosynthesis By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Lipid droplet By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Lipid droplet Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell monolayer-surrounded lipid storage body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Diacylglycerol O-acyltransferase 1		PRO_0000233000
Regions
	Transmembrane	113 – 133	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75BY0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A2E6EC619934ABF1	FASTA	461	52,241
	10 20 30 40 50 60 MQDSMDDSLR EAEGRQDDSE VSSGTTLGSS TPEDSGVTAK LRKKYQMASA LLRRELEELS 70 80 90 100 110 120 VYDAKTAGVS GRSSGSGSGG LALLGGRFHV APLRIPARRR LQTLVVAWHT SSFIYMTVLV 130 140 150 160 170 180 LFLAANPLMW WFMVPYMVYY VWNRSPANGG VVRRYSPRLR SLALWRYYCE YYPISLHKSE 190 200 210 220 230 240 DLAPTFVPDP RGAEPREWKL RLWLWPTRVE LLNLTLQWTR ARPQVATGPR YIFGYHPHGV 250 260 270 280 290 300 GALGAFGAIA TEGCNWSKVF AGIPACLCTL VNQFQIPIYR DYLLGLGCTS VARKNVLKVL 310 320 330 340 350 360 EQNYSVCIVV GGAQEALLSR VGSTELVLNK RKGFIKLALE TGNVNLVPIY AFGETDCFNV 370 380 390 400 410 420 LDTGNESYLR KFQLWIKKTY GFTIPFFFAR GVFNYDFGFL PFRNPINVVV GKPVYVDKRR 430 440 450 460 TNPTMEEIDH YHDLYVQELR NVFDKNKHKF GYAGKELKIV E

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016816 Genomic DNA. Translation: AAS51366.1.
RefSeq	NP_983542.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75BY0.
Genome annotation databases
GeneID	4619674.
GenomeReviews	Gene locus ACR140C in contig AE016816_GR.
KEGG	ago:AGOS_ACR140C.
NMPDR	fig|33169.1.peg.1187.
Organism-specific databases
AGD	ACR140C.
Phylogenomic databases
eggNOG	fuNOG04748.
HOGENOM	HBG561030.
OMA	LTLASRK.
OrthoDB	EOG92JQ84.
Enzyme and pathway databases
BRENDA	2.3.1.20. 279361.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGA1_ASHGO

Accession

Primary (citable) accession number: Q75BY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)