Q75BV4 (LYS1_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Saccharopine dehydrogenase [NAD+, L-lysine-forming]
Short name=SDH
EC=1.5.1.7

Alternative name(s):
Lysine--2-oxoglutarate reductase

Gene names

Name:	LYS1
Ordered Locus Names:	ACR167C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Saccharopine dehydrogenase [NAD+, L-lysine-forming]		PRO_0000199009
Sites
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75BV4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6A6B7154788F1799	FASTA	372	40,974
	10 20 30 40 50 60 MSAILHLRAE TKPMEARAAL TPTTVRTLVS HGFKIYVEES AQSVFEAAEY AAAGAEVVAT 70 80 90 100 110 120 GSWRGAPRER IIVGLKELPE EDTFPLEHTH IQFAHCYKNQ SGWREVLGRF QSGGGLLYDL 130 140 150 160 170 180 EFLQDDRGRR VAAFGYYAGF AGAALGLRDW AWKQTHTDAE DLPAVAPYEN EQALVSEVAA 190 200 210 220 230 240 ACEEAYKKGA RKPRVLVIGA LGRCGSGAVE LLRQCGLHDK HIIRWDIAET ARGGPFPEIA 250 260 270 280 290 300 AADIFINCIY LSQPIAPFIN MELLDRPDRK LRTIVDVSAD TTNPHNPVPV YNVATVFSSP 310 320 330 340 350 360 TVVVPTSQGP KLSVISIDHL PSLLPREASE AFASDLLPSL LQLPERDTAP VWLRAKELFQ 370 QHCERLSKEA RL

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016816 Genomic DNA. Translation: AAS51393.1.
RefSeq	NP_983569.1. NM_208922.1.
3D structure databases
ProteinModelPortal	Q75BV4.
SMR	Q75BV4. Positions 2-372.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75BV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4619701.
GenomeReviews	Gene locus ACR167C in contig AE016816_GR.
KEGG	ago:AGOS_ACR167C.
NMPDR	fig|33169.1.peg.1214.
Organism-specific databases
AGD	ACR167C.
Phylogenomic databases
eggNOG	fuNOG07631.
HOGENOM	HBG331053.
OMA	QFAHCYK.
OrthoDB	EOG408RHS.
PhylomeDB	Q75BV4.
Family and domain databases
InterPro	IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. [Graphical view]
KO	K00290.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_ASHGO

Accession

Primary (citable) accession number: Q75BV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program