Q75BB3 (LYS2_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aminoadipate-semialdehyde dehydrogenase large subunit
EC=1.2.1.31

Alternative name(s):
Alpha-aminoadipate reductase
Short name=Alpha-AR

Gene names

Name:	LYS2
Ordered Locus Names:	ADL346W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	1385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH By similarity.
Catalytic activity	(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.
Cofactor	Binds 1 phosphopantetheine covalently Potential.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NADP Phosphopantetheine
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via aminoadipic acid Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	L-aminoadipate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1385	1385	L-aminoadipate-semialdehyde dehydrogenase large subunit		PRO_0000193148
Regions
	Domain	848 – 917	70	Acyl carrier
Amino acid modifications
	Modified residue	880	1	O-(pantetheine 4'-phosphoryl)serine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75BB3 [UniParc]. Last modified January 9, 2013. Version 2. Checksum: DE96D1018986F338	FASTA	1,385	151,953
	10 20 30 40 50 60 MCAVLEPSMI RWLSEVDNIV VSSLPSDYIP SGPAGVKAES CEVELPGSFG VIDEEDSYIR 70 80 90 100 110 120 LLSAFATLVC RMSGESDVAM YSKANRLLKL AVPPGVAFQQ LRASVTEAVE GTLALPAVDF 130 140 150 160 170 180 DELSALEREK KQLDYYPQYF KVGVVTAADK TKLDQFRYHK FELLLRQVTS SRFEMVYDSE 190 200 210 220 230 240 RFSPDRIGEL GEQLVQFLTL VEAKDDADVY AISLVTSGAS RVLPDPTTDL GWGQFRGAIH 250 260 270 280 290 300 DIFQHHAETR PDRLCVVETG VGQVAARTFT YSAINCASNI VAHYLLARGI RRGDVVMIYS 310 320 330 340 350 360 TRGVDLLVSV LGVLKSGAVF SVIDPAYPPA RQNVYLGVAK PAGLIVIQAA GQLDEAVEAF 370 380 390 400 410 420 IRDNLSLKAR LPALALQTDG AILGGTLPDF HLDTLVPFAS LKNTRTDVVV GPDSNPTLSF 430 440 450 460 470 480 TSGSEGIPKG VLGRHFSLTY YFDWMAKRFG LSEDDKFTML SGIAHDPIQR DMFTPIYLGA 490 500 510 520 530 540 QLLVPQEDDI GTPGRLATWM ATHGATVTHL TPAMGQVLTA DATTPFPSLK RAFFVGDVLT 550 560 570 580 590 600 KRDCARLQSL AENVAIVNMY GSTETQRAVS YFEVPSCSSN PSYLDNLKSI IPAGRGMHNV 610 620 630 640 650 660 QLLIVNRHDR TKLCGIGEVG EIYVRAGGLS EGYRGLPEIN KEKFIDNWFV DAGHWGGLDL 670 680 690 700 710 720 SGDEPWRNYW LGVRDRLYRT GDLGRYLPNG DCECCGRADD QVKIRGFRIE LGEIDTNISQ 730 740 750 760 770 780 YPLCRENITL LRKDQNGEST LISYLVPRSD QKALASFISA VPESIATESI AGSLIKYHKL 790 800 810 820 830 840 INDIRGFLKK RLAGYAIPTL IMVMERLPLN PNGKIDKNKL QFPEPTELDR ASEHFASETL 850 860 870 880 890 900 GLSSFSPLEQ EIRKIWLDLL PTRPAITSSD ESFFDLGGTS ILATRMAIVL RNRLNISLAL 910 920 930 940 950 960 STIFRYPTVK ELAKEISRVR GTISDDKSSN SGTTEYYADA KHVSEAELAS KYESRLSLLP 970 980 990 1000 1010 1020 SGATSAPVYV FLTGVTGFLG CHILADLLNR SRKPYDITVY AHVRASDESS ALQRIKSVCT 1030 1040 1050 1060 1070 1080 AYGLWKNAYA PRIKVVLGNL AEKQFGLPKK AWHDLQEGID VIIHNAALVH WVYPYSKLRE 1090 1100 1110 1120 1130 1140 ANVLSTVNVL NLAAAGKAKY FTFVSSTSAL DTKHYLELSN AAIESGGSGV PEDDDLMGGS 1150 1160 1170 1180 1190 1200 LGLKGGYGQS KWAAEFIIKR AGERGLRGCI LRPGYVTGSP STGASNADDF LLRFLRGCVQ 1210 1220 1230 1240 1250 1260 LGKIPDIEGT VNMVPVDYVA RLATAASFSS SGNTHMMVVN VNAKPRISFR DYLLALKEYG 1270 1280 1290 1300 1310 1320 YQVTSVPYDE WSKALESSSD EENPLYPLLY LVLDDLPKKL RSPELDTTNA KFVLEEDFAR 1330 1340 1350 1360 1370 1380 TNIEPIIITS VSLEVVGSYI SFLHKLGFLE EPAKGSRPLP NISLSDEQIS LIAAVATARS STAKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	Dietrich F.S., Voegeli S., Philippsen P. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 543-570; 575; 578; 587 AND 591.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51573.2.
RefSeq	NP_983749.2. NM_209102.2.
3D structure databases
ProteinModelPortal	Q75BB3.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ADL346W.
Proteomic databases
PRIDE	Q75BB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4619884.
KEGG	ago:AGOS_ADL346W.
Phylogenomic databases
eggNOG	COG3320.
HOGENOM	HOG000191209.
KO	K00143.
OMA	PRDRMYR.
OrthoDB	EOG4BCHW2.
Enzyme and pathway databases
UniPathway	UPA00033; UER00032.
Family and domain databases
Gene3D	1.10.1200.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR014397. L-NH2adipate-semiAld_DH_lsu. IPR013120. Male_sterile_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR010080. Thioester_reductase_dom. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF07993. NAD_binding_4. 1 hit. PF00550. PP-binding. 1 hit. [Graphical view]
PIRSF	PIRSF001617. Alpha-AR. 1 hit.
SUPFAM	SSF47336. ACP_like. 1 hit.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 1 hit. TIGR03443. alpha_am_amid. 1 hit. TIGR01746. Thioester-redct. 1 hit.
PROSITE	PS50075. ACP_DOMAIN. 1 hit. PS00455. AMP_BINDING. 1 hit. PS00012. PHOSPHOPANTETHEINE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS2_ASHGO

Accession

Primary (citable) accession number: Q75BB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	January 9, 2013
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program