Q75BA5 (ALG1_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:	ALG1
Ordered Locus Names:	ADL338C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080252
Regions
	Topological domain	1 – 31	31	Cytoplasmic Potential
	Transmembrane	32 – 52	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	53 – 471	419	Lumenal Potential
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75BA5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9A6CC706672C82E9	FASTA	471	53,663
	10 20 30 40 50 60 MDTSSVTMHT ERACCHQAQR AVAAMLDKAP SWLIWTAVLY VGLPFMLYWA VPYLFYHNKT 70 80 90 100 110 120 KSRRIAIYVL GDLGHSPRIC YHARSFSAAG WEVELCGYLE EQPPKDLLDD PRVTIRALPG 130 140 150 160 170 180 ASNAGKSLGQ TARKVVLQTC HIVRQLWELR GCDYILIQNP PSIPLLPIVA IFKVLTRTRL 190 200 210 220 230 240 ILDWHNFAYT VLQLRVGRFL HPLVLVSYAV EFLFSRMADY HITVTAAMKD YLVQSFLLPA 250 260 270 280 290 300 RRIAVMYDRP GEQFRPLPAG ERGAALAEPF IRGYIPAGFD VQRGDTILVT STSFTLDEDI 310 320 330 340 350 360 NVLFGALKIY ESAAAKFDTT LPRILLFVTG KGPLKGKYME EVRNYKWERC TIHFLWLSAE 370 380 390 400 410 420 DYPRLLQLCD FGVSLHTSTS GLDLPMKVLD MFGSGLPAFV MDYPAIGELV QDRVNGLRFT 430 440 450 460 470 TRRELEQCLI FAIKDEHTRK VLKENALLES KNRWHQRWAS AMSELQVVRQ S

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51581.1.
RefSeq	NP_983757.2. NM_209110.2.
3D structure databases
ProteinModelPortal	Q75BA5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75BA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4619892.
GenomeReviews	Gene locus ADL338C in contig AE016817_GR.
KEGG	ago:AGOS_ADL338C.
NMPDR	fig|33169.1.peg.1402.
Organism-specific databases
AGD	ADL338C.
Phylogenomic databases
eggNOG	fuNOG05192.
HOGENOM	HBG319770.
OMA	LQNPPGL.
OrthoDB	EOG4WWVT5.
PhylomeDB	Q75BA5.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_ASHGO

Accession

Primary (citable) accession number: Q75BA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program