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Q75B12 (ALG11_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

EC=2.4.1.131
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Glycolipid 2-alpha-mannosyltransferase
Gene names
Name:ALG11
Ordered Locus Names:ADL235W
OrganismAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]
Taxonomic identifier284811 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum By similarity.

Catalytic activity

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase group 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
PRO_0000080272

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain34 – 582549Lumenal Potential
Compositional bias139 – 1424Poly-Gly

Amino acid modifications

Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q75B12 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: BE8C12857524068E

FASTA58266,635
        10         20         30         40         50         60 
MESCWLTMES YQAALVVCIV SGLILAVAGY GNVRRLACEF LLKPPKRFRD DICEALLRGP 

        70         80         90        100        110        120 
ENDQKPVLVD FGWRHGAVRR QMLLASAKAS AYSNERHGSK IHISPDDIAR GRSFADALDV 

       130        140        150        160        170        180 
HRRSGRILFG FFHPFCNAGG GGEKVLWKAV ETTLKQSLNN IVVVYTGDCD TTGARILSNV 

       190        200        210        220        230        240 
EHRFGSQLDS ERIVFIFLRH RKWVESRTWP RMTLLGQALG SIVLSIEAAL CCPPDVWCDT 

       250        260        270        280        290        300 
MGYPFGYPFV SWLCRIPIIT YTHYPVVSID MLDKLRMMPE FRNSPTLWAK FLYWRIFMRC 

       310        320        330        340        350        360 
YTFAGSFVDL AVTNSTWTYN HINAIWSRTG NVSIIYPPCS TENLVIENAH DMWDRKHQAV 

       370        380        390        400        410        420 
VIAQFRPEKR HALILRSFSN FVKKTGSNMK LLMLGSTRGQ EDRDYVKKLE QLAYSELAIP 

       430        440        450        460        470        480 
KESLEFITDC KYEKMKKYLQ ESSFGINAMW NEHFGIAVVE YAASGLITLA HASAGPLLDI 

       490        500        510        520        530        540 
IVPWDIEGDK QLERGSDKNR TGFFFKDRSD PDFCKITAEF PTLEELFVRA DQLTDEERLA 

       550        560        570        580 
ISQRAKRCVL HKFSDLKFSE DWAQVVDRTI QLLHTLRNDK VE 

« Hide

References

« Hide 'large scale' references
[1]"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome."
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.
Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]"Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications."
Dietrich F.S., Voegeli S., Kuo S., Philippsen P.
G3 (Bethesda) 3:1225-1239(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016817 Genomic DNA. Translation: AAS51685.1.
RefSeqNP_983861.1. NM_209214.1.

3D structure databases

ProteinModelPortalQ75B12.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING33169.AGOS_ADL235W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAS51685; AAS51685; AGOS_ADL235W.
GeneID4619996.
KEGGago:AGOS_ADL235W.

Phylogenomic databases

eggNOGCOG0438.
HOGENOMHOG000209670.
KOK03844.
OMAHPYANNG.
OrthoDBEOG7673KQ.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR001296. Glyco_trans_1.
[Graphical view]
PfamPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALG11_ASHGO
AccessionPrimary (citable) accession number: Q75B12
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways