Q75AQ8 (ALG10_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
EC=2.4.1.256

Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10
Asparagine-linked glycosylation protein 10
Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10

Gene names

Name:	ALG10
Ordered Locus Names:	ADL138C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.
Catalytic activity	Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ALG10 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase		PRO_0000215450
Regions
	Transmembrane	27 – 47	21	Helical; Potential
	Transmembrane	112 – 132	21	Helical; Potential
	Transmembrane	134 – 154	21	Helical; Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Transmembrane	197 – 217	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Transmembrane	317 – 337	21	Helical; Potential
	Transmembrane	379 – 399	21	Helical; Potential
	Transmembrane	413 – 433	21	Helical; Potential
	Transmembrane	439 – 459	21	Helical; Potential
	Transmembrane	473 – 493	21	Helical; Potential
Amino acid modifications
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q75AQ8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 23DFCFDFBD8825E4	FASTA	509	59,103
	10 20 30 40 50 60 MGGEATASEK ELQEREDQLS QQIQDEIVMG CVVNLALWPV LLGAAAYVAY KYNCSWVPYP 70 80 90 100 110 120 FIDEKFHVGQ TVRYLAGRWR EWDSKITTPP GLYVIGWAVQ RTVGLLVGWN TLSLLRLSNV 130 140 150 160 170 180 IGGLVVWPWF VLRPLYFFNA LAFWPATLSV FPLLTSYYFL YYTDVWSTIL IVGSLTLAVT 190 200 210 220 230 240 VPFGERASIW ASAICGLLSC LFRLTNIVWN AFVLVVVLER RTMIHKGFNS LRINNYLKLI 250 260 270 280 290 300 IHGIENWNSL VLPYAVNFAL FLIFLLYNGS VTLGDKSSHV AGFHLVQMFY CLLFITFFSV 310 320 330 340 350 360 PVWFCRSFLL NYVSRTVVYP IWTIFEILGI MMIIRFFTVV HPYLLADNRH IAFYLFKKLI 370 380 390 400 410 420 GRNRFLKYFV MAPIYHFSTF VYLEAVRPTV FFFHPILPIE VKSPVDLPLQ FTHISWSALI 430 440 450 460 470 480 ICTLLTVVPS PLFEPRYYIL PYIFWRIFLL VSPEPFFAVP TEMTYRFGNT RRLAVEFLWF 490 500 ILINAITIVI FATNAFAWET EASLQRIIW

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51782.1.
RefSeq	NP_983958.2. NM_209311.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75AQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4620100.
GenomeReviews	Gene locus ADL138C in contig AE016817_GR.
KEGG	ago:AGOS_ADL138C.
NMPDR	fig|33169.1.peg.1603.
Organism-specific databases
AGD	ADL138C.
Phylogenomic databases
eggNOG	fuNOG05358.
HOGENOM	HBG714502.
OMA	LLTWPYI.
OrthoDB	EOG47WRXP.
PhylomeDB	Q75AQ8.
Family and domain databases
InterPro	IPR016900. Alpha1_2_glucosyltferase_Alg10. IPR007006. Glycosyltransferase_ALG10. [Graphical view]
KO	K03850.
PANTHER	PTHR12989. DIE2_ALG10. 1 hit.
Pfam	PF04922. DIE2_ALG10. 1 hit. [Graphical view]
PIRSF	PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALG10_ASHGO

Accession

Primary (citable) accession number: Q75AQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program