Reviewed, UniProtKB/Swiss-Prot Q75AL5 (JHD1_ASHGO)

Names and origin

Protein names

Recommended name:
    JmjC domain-containing histone demethylation protein 1
    EC=1.14.11.27
Alternative name(s):
    [Histone-H3]-lysine-36 demethylase 1

Gene names

Name:	JHD1
Ordered Locus Names:	ADL088W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code By similarity.
Catalytic activity	Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2. Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subcellular location	Nucleus By similarity.
Domain	The JmjC domain mediates the demethylation activity By similarity.
Sequence similarities	Belongs to the JHDM1 histone demethylase family. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Iron Metal-binding Zinc
Molecular function	Chromatin regulator Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone demethylase activity (H3-K36 specific) Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	JmjC domain-containing histone demethylation protein 1		PRO_0000226791
Regions
	Domain	245 – 404	160	JmjC
	Zinc finger	28 – 83	56	PHD-type; degenerate
Sites
	Metal binding	300	1	Iron; catalytic By similarity
	Metal binding	302	1	Iron; catalytic By similarity
	Metal binding	372	1	Iron; catalytic By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	317	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75AL5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 53A9F8861DCACDC7	FASTA	486	55,076
	10 20 30 40 50 60 MLQQVTSTTV TGADIQLRAK LLLFSMTDDQ CVYCKGKMEH RMWVQCEACP QWVHVQCIPE 70 80 90 100 110 120 ECLSGGEYPS RSSDIAAFEC SAHGTARARL ALKGKRRRVE AKEEPERAGT RRYRLRKRGP 130 140 150 160 170 180 LDYIALNEGQ DVRLRHEHPH RAAFQGCFTK WSGLGRTVTS AELQQSFAEL REPVLVADPE 190 200 210 220 230 240 HSGMQTPAMD EQVLADVLGA DYSLDVMDVQ SQQNERWTMG QWKEYMHTAR GVRDRIRNVI 250 260 270 280 290 300 SLEVSHVPEF GQRIRRPRAV EDNDLVDLVW PVQPAPEIGA KPKVQKYVLM SAANAYTDFH 310 320 330 340 350 360 LDFAGTSVYY SLLRGAKQFL LFPPTPANLG AYKAWCADDN QGLIFLGDRL QDGVLFSLRP 370 380 390 400 410 420 GDLFMIPSGF IHAVYTPEDS FVVGGNYLCL RDLSTHIRIV RIEQETQVPK KFTFPKFERV 430 440 450 460 470 480 MGLTAEWLLE GLPERLQLIT HEHAVALLDY LRDTRLKYKP AHYHTKSTML ASLEKALEGC EPASGP

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51832.1.
RefSeq	NP_984008.1.
3D structure databases
SMR	Q75AL5. Positions 136-436.
ModBase	Search...
Genome annotation databases
GeneID	4620150.
GenomeReviews	Gene locus ADL088W in contig AE016817_GR.
KEGG	ago:AGOS_ADL088W.
NMPDR	fig|33169.1.peg.1653.
Organism-specific databases
AGD	ADL088W.
Phylogenomic databases
eggNOG	fuNOG04730.
HOGENOM	HBG398146.
OMA	ISLEVSH.
OrthoDB	EOG9H47M1.
Enzyme and pathway databases
BRENDA	1.14.11.27. 279361.
Family and domain databases
InterPro	IPR003347. TF_JmjC_AAH. IPR001965. Znf_PHD. [Graphical view]
SMART	SM00558. JmjC. 1 hit. SM00249. PHD. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. PS01359. ZF_PHD_1. False negative. PS50016. ZF_PHD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JHD1_ASHGO

Accession

Primary (citable) accession number: Q75AL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)