Q75AL4 (NCB5R_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 1
EC=1.6.2.2

Alternative name(s):
Microsomal cytochrome b reductase

Gene names

Name:	CBR1
Ordered Locus Names:	ADL087W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	NADH-cytochrome b5 reductase 1		PRO_0000330143
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Domain	41 – 145	105	FAD-binding FR-type
	Nucleotide binding	125 – 142	18	FAD By similarity
	Nucleotide binding	151 – 183	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75AL4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 61CACAC5DF35FC5C	FASTA	287	31,718
	10 20 30 40 50 60 MDYKFEALVT ALVLAVSFIF IYGKFSGAKQ SQPAVKTTLN KDWQEFSLLT KTVLTHNTAI 70 80 90 100 110 120 YRFGLPEADA VLGLPIGQHI SISGVIDGKE MLRSYTPTSL DSDATGYFEL LVKSYEKGNI 130 140 150 160 170 180 SKMLAELAIG DRIKVRGPKG FYHYEPNMYK EIGMIAGGTG ISPMYQIIRA IFSNPRDKTR 190 200 210 220 230 240 VCLVYGNQTK DDILLKPELD AMVAAKPDQF KILYMLDKVA EGEQWEGKLG YITEAIMREH 250 260 270 280 LPAPSSSAQL LLCGPPPMVS SAKRIAVSLG FEKAKPISKK GDQVFAF

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51833.1.
RefSeq	NP_984009.1. NM_209362.1.
3D structure databases
HSSP	HSSP built from PDB template 1QX4 based on UniProtKB P20070.
ProteinModelPortal	Q75AL4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4620151.
GenomeReviews	Gene locus ADL087W in contig AE016817_GR.
KEGG	ago:AGOS_ADL087W.
NMPDR	fig|33169.1.peg.1654.
Organism-specific databases
AGD	ADL087W.
Phylogenomic databases
eggNOG	fuNOG05226.
HOGENOM	HBG591994.
OMA	SSENING.
OrthoDB	EOG4FFH9R.
PhylomeDB	Q75AL4.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_ASHGO

Accession

Primary (citable) accession number: Q75AL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program