Q75AB5 (MPI_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

Name:	PMI1
Synonyms:	PMI40
Ordered Locus Names:	ADR003C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Mannose-6-phosphate isomerase		PRO_0000194239
Sites
	Active site	300	1	By similarity
	Metal binding	109	1	Zinc By similarity
	Metal binding	111	1	Zinc By similarity
	Metal binding	136	1	Zinc By similarity
	Metal binding	281	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75AB5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7755EF1BC22F072A	FASTA	429	47,530
	10 20 30 40 50 60 MSAKLFRLDA GYQQYDWGKI GSSSAVAQYA ANSDPSVQIE EDKPYAELWM GTHHKVPSRH 70 80 90 100 110 120 HDTKVALSDL IAANPEGMLG SGNVEKFHSR KDLPFLFKVL SIEKVLSIQA HPDKNLGRRL 130 140 150 160 170 180 HIQDPKNYPD DNHKPEMAIA ISDFEGFCGF KPLEELAEEL QRIPEFRSLV GEDIAAQFCA 190 200 210 220 230 240 GIKVGAQEGS EEDKSNRKLL QSVFSNVMNA SDDVIASHAR HLVERAKQSP ADFNNETLPA 250 260 270 280 290 300 LILRLNEQFP EDVGLFCGCL MLNHCVLKAG ESIFLRAKDP HAYITGDIIE CMAASDNVVR 310 320 330 340 350 360 AGFTPKFKDV KNLVEMLTYS YDSVEDQKMK LLPFARSSGS GDSILYNPPI EEFAVLQTNF 370 380 390 400 410 420 HGSTGTHHFD GLDGPSIVIT TNGSGYIQSG DVRLKAEAGY VFFIAPHAEV DLQTEDSNFT TYRAFVEPN

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS51923.1.
RefSeq	NP_984099.1. NM_209452.1.
3D structure databases
ProteinModelPortal	Q75AB5.
SMR	Q75AB5. Positions 2-427.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ADR003C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS51923; AAS51923; AGOS_ADR003C.
GeneID	4620247.
KEGG	ago:AGOS_ADR003C.
Phylogenomic databases
eggNOG	COG1482.
HOGENOM	HOG000241277.
KO	K01809.
OMA	ACTPHAY.
OrthoDB	EOG4TF3V5.
PhylomeDB	Q75AB5.
Enzyme and pathway databases
UniPathway	UPA00126; UER00423.
Family and domain databases
Gene3D	2.60.120.10. 3 hits.
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_ASHGO

Accession

Primary (citable) accession number: Q75AB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program