Q75A17 (TRM10_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	TRM10
Ordered Locus Names:	ADR110C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060509

Sequences

	Sequence		Length	Mass (Da)
	Q75A17 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3C2C10CDBEDE032F	FASTA	296	34,049
	10 20 30 40 50 60 MTPETNNDET LSRPKPRAAL PPVPEGMSKS QWKKQWKKEQ FELNKPLYAK IRKEKKQKAR 70 80 90 100 110 120 EQRRERLQKA LEENGGEIPE ELRRTPRVNV NQKDSGIKVI IDCAFDELMN EKEIVSLSTQ 130 140 150 160 170 180 ITRAYSANKR ENHFADVKVT SFNKRLKERF DCGLKGANYD AWKHFEFTDE SALPTTNAVY 190 200 210 220 230 240 LTADTDETLE TLEPGTTYIV GGIVDKNRHK ALCYNKAKEL GIPTRRLPIG EYIKLCGRKV 250 260 270 280 290 LTTTHVIQIM LRYFDNHDWK EAFESVLPAR KLAELADHAQ ESNSSSPAEE QDAQDI

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS52030.1.
RefSeq	NP_984206.1. NM_209559.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q75A17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4620377.
GenomeReviews	Gene locus ADR110C in contig AE016817_GR.
KEGG	ago:AGOS_ADR110C.
NMPDR	fig|33169.1.peg.1851.
Organism-specific databases
AGD	ADR110C.
Phylogenomic databases
eggNOG	fuNOG04600.
HOGENOM	HBG717479.
OMA	SNVERKQ.
OrthoDB	EOG48D43Z.
PhylomeDB	Q75A17.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. IPR016653. tRNA_m1G_mtfrase_met. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF016323. tRNA_m1G_mtfrase_met. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM10_ASHGO

Accession

Primary (citable) accession number: Q75A17

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program