Reviewed, UniProtKB/Swiss-Prot Q759W0 (CP51_ASHGO)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 51
    EC=1.14.13.70
Alternative name(s):
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    Lanosterol 14-alpha demethylase
    P450-14DM

Gene names

Name:	ERG11
Synonyms:	CYP51
Ordered Locus Names:	ADR162W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP(+) + 4 H(2)O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	MembranePotential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Cytochrome P450 51		PRO_0000052002
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q759W0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BE674CB09ABD67DA	FASTA	529	60,506
	10 20 30 40 50 60 MSESLLQTVV AYVELVLHHF MALSWTQQLS IVIVAPFIYS LVWQTLYSFR KDRVPLVPFM 70 80 90 100 110 120 VPWVGSALAY GRAPYEFFGK CQQKYGDVFA FMLLGRVMTV YLGTKGHEFI LNAKLAEVSA 130 140 150 160 170 180 EEAYTKLTTP VFGEGVVYDC PNHRLMEQKK FCKNALSTEA FRRYVPMVMD EVRKYLRTSK 190 200 210 220 230 240 HFMMNERSSG VVNVMETQPE MTIFTASRSL LGAEMHSMLD ADFAYLYADL DKGFTPLNFV 250 260 270 280 290 300 FRDLPLDNYR RRDNAQRTIS STYMKVIERR RKNNDVQDRD LIDALMTSAQ YKDGVKMTDQ 310 320 330 340 350 360 QIANLLIGVL MGGQHTSAAT SAWVLLHLAE RPDIQEELYE EQMRVLDGGA KELTYELLQE 370 380 390 400 410 420 MPLLNQVIKE TLRMHHPLHS LFRKVTRDMP VPNTSYVIPK DHYVLASPGF CHLSEEYFPN 430 440 450 460 470 480 AKEFNPHRWD NDAASSVSTG EKVDYGFGAI SKGVSSPYLP FGGGRHRCIG EGFAYMQLGT 490 500 510 520 IFSVVVRSMK WHFPADMKGV PNPDFTSMVT LPSEPCRIAW ERRVPDQII

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016817 Genomic DNA. Translation: AAS52083.1.
RefSeq	NP_984259.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4620421.
KEGG	ago:AGOS_ADR162W.
NMPDR	fig|33169.1.peg.1904.
Organism-specific databases
AGD	ADR162W.
Phylogenomic databases
HOGENOM	Q759W0.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_ASHGO

Accession

Primary (citable) accession number: Q759W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)