Reviewed, UniProtKB/Swiss-Prot Q759G5 (GPD_ASHGO)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+]
    EC=1.1.1.8

Gene names

Name:	GPD
Ordered Locus Names:	ADR311C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138085
Regions
	Nucleotide binding	79 – 84	6	NAD By similarity
	Region	348 – 349	2	Substrate binding By similarity
Sites
	Active site	283	1	Proton acceptor By similarity
	Binding site	167	1	NAD By similarity
	Binding site	190	1	NAD; via amide nitrogen By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	223	1	NAD; via amide nitrogen By similarity
	Binding site	348	1	NAD By similarity
	Binding site	377	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q759G5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 014C3D1A7F251630	FASTA	424	46,646
	10 20 30 40 50 60 MVNTTALIRS PTFVAQFRVL KQVNRGLFSQ TSYARTHART SYTHTMAGTD RLQQTSQILS 70 80 90 100 110 120 RSTSSEIRLE RPFKVTVIGS GNWGTTIAKV VAENTQEYPQ LFERRVDMWV FEEQIEGRKL 130 140 150 160 170 180 TEIINEQHEN VKYLPGITLP ENLVANPSVA AAAADADVLV FNIPHQFLGR IVEQLKGHVK 190 200 210 220 230 240 PGARAISCLK GFSVGKDGVQ LLSTYIEEHL HIPCGALSGA NLAPEVAKGN WSETTVAYTL 250 260 270 280 290 300 PQDFRGTGKD VDHAVLKKLF HRPYFHVNVV DDVAGISVAG ALKNVVALAC GFVLGLGWGN 310 320 330 340 350 360 NAAAAVQRVG LSEMIKFARM FFPESKVETF YQESAGVADL ITTCAGGRNV RIGRAMAETG 370 380 390 400 410 420 KSAQELEKEL LNGQSSQGIY TTQEVHEWLQ QCGKKDEFPL FEAVYKIVYE GVPMSKLPDM LEDA

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016817 Genomic DNA. Translation: AAS52231.1.
RefSeq	NP_984407.1.
3D structure databases
HSSP	HSSP built from PDB template 1JDJ based on UniProtKB P90551.
ModBase	Search...
Genome annotation databases
GeneID	4620680.
KEGG	ago:AGOS_ADR311C.
NMPDR	fig|33169.1.peg.2052.
Organism-specific databases
AGD	ADR311C.
Phylogenomic databases
HOGENOM	Q759G5.
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD_ASHGO

Accession

Primary (citable) accession number: Q759G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)