Q759A9 (KPYK_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	PYK1
Ordered Locus Names:	ADR368W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway pyruvate metabolic process Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Pyruvate kinase		PRO_0000112107
Sites
	Metal binding	52	1	Potassium By similarity
	Metal binding	54	1	Potassium By similarity
	Metal binding	85	1	Potassium By similarity
	Metal binding	86	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	243	1	Magnesium Potential
	Metal binding	267	1	Magnesium By similarity
	Binding site	50	1	Substrate By similarity
	Binding site	266	1	Substrate; via amide nitrogen By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity
	Binding site	299	1	Substrate By similarity
	Binding site	338	1	ADP Potential
	Site	241	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q759A9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C474BC449337CDD1	FASTA	501	54,808
	10 20 30 40 50 60 MESRLGWLTR LNYDTGSALR RTSIIGTIGP KTNNPETLVE LRKAGLNIVR MNFSHGSYEY 70 80 90 100 110 120 HQSVIDNARK SEELYPGRPL AIALDTKGPE IRTGTTVDNV DYPIPPNHEM IFTTDDQYAK 130 140 150 160 170 180 SCDDKVMFVD YKNITKVIEK GRVIYVDDGV LSFEVLEVVD DKTLKVKSLN AGKICSHKGV 190 200 210 220 230 240 NLPGTDVDLP ALSEKDKSDL RFGVKNGVHM VFASFIRTAG DILTIREILG EDGKDIKIIA 250 260 270 280 290 300 KIENQQGVNN FDEILKVTDG VMVARGDLGI EIPAPQVFAV QKMLIAKCNL AGKPVICATQ 310 320 330 340 350 360 MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGI YPINAVKTMA ETALIAEQAI 370 380 390 400 410 420 PYVPTYDDLR NLTPKPTPTS ETIAAAAVAA TFEQKAKAIV VLSTSGDTTR LVSKYKPNVP 430 440 450 460 470 480 IVMVTRNPRA ARYCHLHRGV FPFVYEQEAA DEWTDDVEAR LNFGVEKSKE LGILSEGDTI 490 500 VTIQGFASGV GHSNTLRVLT V

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016817 Genomic DNA. Translation: AAS52288.1.
RefSeq	NP_984464.1. NM_209817.1.
3D structure databases
ProteinModelPortal	Q759A9.
SMR	Q759A9. Positions 3-501.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ADR368W.
Proteomic databases
PRIDE	Q759A9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS52288; AAS52288; AGOS_ADR368W.
GeneID	4620629.
KEGG	ago:AGOS_ADR368W.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
KO	K00873.
OMA	NSGYTAR.
OrthoDB	EOG43XZC1.
PhylomeDB	Q759A9.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_ASHGO

Accession

Primary (citable) accession number: Q759A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program