Q757Q6 (GLGB_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan-branching enzyme
EC=2.4.1.18

Alternative name(s):
Glycogen-branching enzyme

Gene names

Name:	GLC3
Ordered Locus Names:	AEL044W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	1,4-alpha-glucan-branching enzyme		PRO_0000188777
Sites
	Active site	355	1	Nucleophile By similarity
	Active site	415	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q757Q6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B41AE8250252DBF4	FASTA	703	80,263
	10 20 30 40 50 60 MAGVPDNVKG VVELDPWLAP YGDILSARRF LADKWRHDIE HAVPGGRRSL VEFARDAYKS 70 80 90 100 110 120 YGLHADAQSK SITYREWAPN ATRAFLVGDF NGWDETSHEL QNKDEFGVFT GVFGPGADGD 130 140 150 160 170 180 FMIPHDSRVK VVFELADGSR IHRLPAWIKR ATQPSKETAK EWGPSYEARF WNPASPYKFK 190 200 210 220 230 240 HERPRLDPNV ESLRIYEAHV GISTPEPRVG SYSEFTKDVL PRIRDLGYNA IQLMAIMEHA 250 260 270 280 290 300 YYASFGYQVT NFFAVSSRYG TPEELKELID TAHGMGIQVL LDVVHSHASK NVSDGLNMFD 310 320 330 340 350 360 GTDYQYFHSI SSGRGEHPLW DSRLFNYGSF EVQRFLLANL AFYIDVYQFD GFRFDGVTSM 370 380 390 400 410 420 LYHHHGVGER GAFSGDYNEY LSDHSGVDHE ALAYLMLAND LIHDMLPANG VTVAEDVSGY 430 440 450 460 470 480 PTLCLPRSVG GCGFDYRLAM ALPDMWIKLL KESKDEDWSM GHIVYTLVNR RYKEKVVAYA 490 500 510 520 530 540 ESHDQALVGD KTLAFWMMDA AMYTDMTVLK ELTPVVDRGI ALHKLIRLIT HSLGGESYLN 550 560 570 580 590 600 FEGNEFGHPE WLDFPNANNG DSYQYARRQF NLVDDGLLRY KHLYAFDKAM QEAEGKHKWL 610 620 630 640 650 660 NTPQAYVSLK HETDKVISFE RNGLVFIFNF HPTQSFTDYR IGVDEAGAYR IILNSDREEF 670 680 690 700 GGHRRIEEEN SVFHTTDLEW NGRRNFIQVY LPSRTALVLA RNP

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016818 Genomic DNA. Translation: AAS52641.1.
RefSeq	NP_984817.1. NM_210171.1.
3D structure databases
ProteinModelPortal	Q757Q6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q757Q6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4621011.
GenomeReviews	Gene locus AEL044W in contig AE016818_GR.
KEGG	ago:AGOS_AEL044W.
NMPDR	fig|33169.1.peg.2462.
Organism-specific databases
AGD	AEL044W.
Phylogenomic databases
eggNOG	fuNOG05596.
HOGENOM	HBG302308.
OMA	RWDSSGI.
OrthoDB	EOG47M562.
PhylomeDB	Q757Q6.
Family and domain databases
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ASHGO

Accession

Primary (citable) accession number: Q757Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program