Reviewed, UniProtKB/Swiss-Prot Q757J8 (IPYR_ASHGO)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	IPP1
Ordered Locus Names:	AER015C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Inorganic pyrophosphatase		PRO_0000137579
Sites
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	153	1	Magnesium 1 By similarity
	Binding site	79	1	Inorganic pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q757J8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D503790D8F2DBECF	FASTA	287	32,278
	10 20 30 40 50 60 MSYTTRQIGA KNTLDYRVFI EKAGKVVSPF HDIPLYADEE NQIFNMVVEI PRWTNAKLEI 70 80 90 100 110 120 TKEEAMNPII QDTKKGKLRY VRNCFPHHGY IHNYGAFPQT WEDPNVAHPE TKAYGDNDPL 130 140 150 160 170 180 DVLEIGETIA YTGQVKQVKV LGVMALLDEG ETDWKIIVID VHDPLAPKLN DIEDVEKHLP 190 200 210 220 230 240 GLLRATNEWF RIYKIPDGKP ENQFAFSGEA KNRKYALDVI RECHEAWCQL VAGKAADDKK 250 260 270 280 VSLANSTLQE SVAYAPEVAA AVPAANPLPD APIDKSIDKW FFISGSA

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016818 Genomic DNA. Translation: AAS52699.1.
RefSeq	NP_984875.1.
3D structure databases
SMR	Q757J8. Positions 2-285.
ModBase	Search...
Genome annotation databases
GeneID	4621075.
KEGG	ago:AGOS_AER015C.
NMPDR	fig|33169.1.peg.2520.
Organism-specific databases
AGD	AER015C.
Phylogenomic databases
HOGENOM	Q757J8.
OMA	Q757J8. WFFISGS.
Enzyme and pathway databases
BRENDA	3.6.1.1. 279361.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_ASHGO

Accession

Primary (citable) accession number: Q757J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)