Reviewed, UniProtKB/Swiss-Prot Q756G9 (GCN5_ASHGO)

Names and origin

Protein names

Recommended name:
    Histone acetyltransferase GCN5
    EC=2.3.1.48

Gene names

Name:	GCN5
Ordered Locus Names:	AER297C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acetylates histone H2B to form H2BK11ac and H2BK16ac, histone H3 to form H3K14ac, with a lower preference histone H4 to form H4K8ac and H4K16ac, and contributes to H2A.Z acetylation. Acetylation of histones gives a specific tag for epigenetic transcription activation By similarity.
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the GCN5 family. Contains 1 bromo domain. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Bromodomain
Molecular function	Activator Acyltransferase Chromatin regulator Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Histone acetyltransferase GCN5		PRO_0000211195
Regions
	Domain	113 – 268	156	N-acetyltransferase
	Domain	357 – 427	71	Bromo
Sites
	Site	186	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q756G9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9BA78D49039CAC66	FASTA	452	52,414
	10 20 30 40 50 60 MSMPLKRRNK QSQGNEPKKI KVEEQEAEEK ISEDIPVTDL KEPEALADIQ STSGGDEVSE 70 80 90 100 110 120 GAQGDADPAE KSVGGLKEEV EDEEKGIVKF MFDGVEYKFR ERPSVIEEKE GKIEFRVVNN 130 140 150 160 170 180 DNTRENMMVL TGLKNIFQKQ LPKMPKEYIA RLVYDRSHLS MAVIRKPLTV VGGITYRPFE 190 200 210 220 230 240 KGEFAEIVFC AISSTEQVRG YGAHLMNHLK DYVRATTNIK YFLTYADNYA IGYFKKQGFT 250 260 270 280 290 300 KEITLDKSVW MGYIKDYEGG TLMQCFMLPR IRYLDAAKIL LLQEAAIQRK IRTISRSHIV 310 320 330 340 350 360 RPGLRQFEDL DNIEPIDPMS VPGLREAGWT PEMDELAQRP KRGPHYATMQ NVLTELQNHA 370 380 390 400 410 420 AAWPFLQPVN RDEVPDYYEF IKEPMDLSTM EIKLENNRYE KMEDFIYDAR LIFNNCRAYN 430 440 450 GENTSYFKYA NRLEKFFNTK MKEIPEYSHL LD

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016818 Genomic DNA. Translation: AAS52978.1.
RefSeq	NP_985154.1.
3D structure databases
SMR	Q756G9. Positions 112-275, 342-451.
ModBase	Search...
Protein-protein interaction databases
STRING	Q756G9.
Genome annotation databases
GeneID	4621367.
GenomeReviews	Gene locus AER297C in contig AE016818_GR.
KEGG	ago:AGOS_AER297C.
NMPDR	fig|33169.1.peg.2799.
Organism-specific databases
AGD	AER297C.
Phylogenomic databases
eggNOG	fuNOG05844.
HOGENOM	HBG749648.
OMA	GLKNIFQ.
OrthoDB	EOG9BP2TZ.
Enzyme and pathway databases
BRENDA	2.3.1.48. 279361.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001487. Bromodomain. IPR018359. Bromodomain_CS. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit. G3DSA:1.20.920.10. Bromodomain. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. PF00439. Bromodomain. 1 hit. [Graphical view]
PRINTS	PR00503. BROMODOMAIN.
SMART	SM00297. BROMO. 1 hit. [Graphical view]
PROSITE	PS00633. BROMODOMAIN_1. 1 hit. PS50014. BROMODOMAIN_2. 1 hit. PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCN5_ASHGO

Accession

Primary (citable) accession number: Q756G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)