Reviewed, UniProtKB/Swiss-Prot Q756F2 (PPN1_ASHGO)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	PPN1
Ordered Locus Names:	AER313C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole is required for activation By similarity.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Endopolyphosphatase		PRO_0000058543
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 43	19	Signal-anchor for type II membrane protein Potential
	Topological domain	44 – 594	551	Vacuolar Potential
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	447	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q756F2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 65888DA861367AC1	FASTA	594	67,713
	10 20 30 40 50 60 MAAEEGRRLQ MKNGEGVGRS GRPEWALWIA ALMAGALWWT VHSDAVALLS KACGKDAATT 70 80 90 100 110 120 PRRRRALRGR FLHITDIHPD PLYVEGSSIA GACHGDPARD ASDRAGRFGD AMGGCDAPMD 130 140 150 160 170 180 LMNYTLAWVA THLRDEIDFV VWTGDNARHD NDRRQPRTAE QILHMNAQVA DQFYELFQQQ 190 200 210 220 230 240 RDGDTMQGVP VVPSLGNNDV FPHNMFSLGP TLMTRQLQRI WAKFIPEEQR QAFEHDTSFF 250 260 270 280 290 300 VEVIPDRLAV ISFNTLYFFK SNPLVDNCND KAQPGHALLL WLGSVLEEMR ARNMKVWLSG 310 320 330 340 350 360 HVPPIPKNFE GSCYNKLAWW TYEYRDVIIG GLYGHMNVDH FYPADGKAAK KSIKRRGKAR 370 380 390 400 410 420 TKERNTQEQP LDILDDSVDN LFSGRGAMPL RKEKYMRRLF KDVYCPIAKR LEDKGKQEQS 430 440 450 460 470 480 YERYSMVHVA ASVIPTFNPG FRVWEYNLTG IDNAVAPAPW TRLVRRAQQI LSPLRKKKHG 490 500 510 520 530 540 PDRTLPPKKP KHLAAGPGHI PQALTPIRFT QYYADLAEIN KDYARELEAG RSHDEAAAAA 550 560 570 580 590 FRYKIEYTSE DEPYPMESLL VRDYVKLASK LTEDRTAWKT YVRRAFVSSG YRDN

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016818 Genomic DNA. Translation: AAS52993.1.
RefSeq	NP_985169.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4621382.
KEGG	ago:AGOS_AER313C.
NMPDR	fig|33169.1.peg.2814.
Organism-specific databases
AGD	AER313C.
Phylogenomic databases
HOGENOM	Q756F2.
OMA	Q756F2. GGLYGHM.
Enzyme and pathway databases
BRENDA	3.6.1.10. 279361.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. IPR004843. M-pesterase. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF027093. EndopolyPtase_N1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPN1_ASHGO

Accession

Primary (citable) accession number: Q756F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)