Reviewed, UniProtKB/Swiss-Prot Q756E1 (DOT1_ASHGO)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
Ordered Locus Names:	AER326C

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270603
Regions
	Region	389 – 391	3	S-adenosyl-L-methionine binding By similarity
	Region	451 – 452	2	S-adenosyl-L-methionine binding By similarity
	Motif	385 – 396	12	SAM-binding motif 1 By similarity
	Motif	466 – 475	10	SAM-binding motif 2 By similarity
Sites
	Binding site	367	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	414	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q756E1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: ACE258C91ED8ED35	FASTA	575	63,831
	10 20 30 40 50 60 MANNMNFVRV VANHNSQPSR HIYIKAPVTM AQTVRGADAA GNQNQNAGKG GVETTQLVGG 70 80 90 100 110 120 QRPGAARKAN RMLLGLLEDS ARYDMKSDYS LQDTWLRKRR ATAQKTAKAE DKSVDTKKTR 130 140 150 160 170 180 AAGKKTKPSE AGPDAKARSG MTSAPSSSST DAAAPAATGK ARNGKARAAS SVTRNTVLAN 190 200 210 220 230 240 ASPFASPSED VIELQHQFFR ITDHQDCVNR EITSSLLLTP KDVADQQIVR LHFILYPDVS 250 260 270 280 290 300 EEYKVTFGHP NNGVFNPIHE IGKCIKYAVE IYFPKTFKKK GSKLLQQLSK AYASMNQSAF 310 320 330 340 350 360 VEAVEQYNDL VRAIPQDQVN EHLQQTKKIP RAFIHDILQM AYSRCVLPNV NGLKEYRSFS 370 380 390 400 410 420 NFVYGELLPT FLSTVYQQCD LKPGQIFIDL GSGVGNCVVQ ASLEYGCALS FGCEIMKNAS 430 440 450 460 470 480 ALAKSQLKEL EERCALWGVD LKPIEFSLRK SFIDNERVNE LLPQCDVLLI NNFIFDTKLN 490 500 510 520 530 540 QAVEKLIQGL KPGCKIITLK NLRPSGYTIN FDDVENILNR LKVEKFTLPE DSVSWTYRGG 550 560 570 GEYFISTVQA DIDESVFHSY AKGRVRGMRP AKYTK

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016818 Genomic DNA. Translation: AAS53006.1.
RefSeq	NP_985182.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q756E1.
Genome annotation databases
GeneID	4621395.
GenomeReviews	Gene locus AER326C in contig AE016818_GR.
KEGG	ago:AGOS_AER326C.
NMPDR	fig|33169.1.peg.2827.
Organism-specific databases
AGD	AER326C.
Phylogenomic databases
HOGENOM	Q756E1.
OMA	LINNFIF.
Enzyme and pathway databases
BRENDA	2.1.1.43. 279361.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_ASHGO

Accession

Primary (citable) accession number: Q756E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)