Q756B7 (GUAA_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	GUA1
Ordered Locus Names:	AER350W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	GMP synthase [glutamine-hydrolyzing]		PRO_0000286143
Regions
	Domain	13 – 202	190	Glutamine amidotransferase type-1
	Domain	203 – 400	198	GMPS ATP-PPase
	Nucleotide binding	231 – 237	7	ATP By similarity
Sites
	Active site	89	1	For GATase activity By similarity
	Active site	176	1	For GATase activity By similarity
	Active site	178	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q756B7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D2564C3BF94DE6B9	FASTA	525	58,202
	10 20 30 40 50 60 MAAVEQVSSV FDTILVLDFG SQYSHLITRR LREFNVYAEM LPCTQKISEL GWKPKGVILS 70 80 90 100 110 120 GGPYSVYAAD APHVDRAVFE LGVPILGICY GLQELAWIAG AEVGRGEKRE YGRATLHVED 130 140 150 160 170 180 SACPLFNNVD SSTVWMSHGD KLHALPADFH VTATTENSPF CGIAHDSKPI FGIQFHPEVT 190 200 210 220 230 240 HSSQGKTLLK NFAVEICQAA QTWTMENFID TEIQRIRTLV GPTAEVIGAV SGGVDSTVAA 250 260 270 280 290 300 KLMTEAIGDR FHAILVDNGV LRLNEAANVK KILGEGLGIN LTVVDASEEF LTKLKGVTDP 310 320 330 340 350 360 EKKRKIIGNT FIHVFEREAA RIQPKNGEEI EFLLQGTLYP DVIESISFKG PSQTIKTHHN 370 380 390 400 410 420 VGGLLDNMKL KLIEPLRELF KDEVRHLGEL LGISHELVWR HPFPGPGIAI RVLGEVTKEQ 430 440 450 460 470 480 VEIARKADHI YIEEIRKAGL YNKISQAFAC LLPVKSVGVM GDQRTYDQVI ALRAIETTDF 490 500 510 520 MTADWYPFEH EFLKHVASRI VNEVEGVARV TYDITSKPPA TVEWE

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016818 Genomic DNA. Translation: AAS53030.1.
RefSeq	NP_985206.1. NM_210560.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ProteinModelPortal	Q756B7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q756B7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4621421.
GenomeReviews	Gene locus AER350W in contig AE016818_GR.
KEGG	ago:AGOS_AER350W.
NMPDR	fig|33169.1.peg.2851.
Organism-specific databases
AGD	AER350W.
Phylogenomic databases
eggNOG	fuNOG07551.
HOGENOM	HBG391775.
OMA	DGRTYEY.
OrthoDB	EOG4WWVST.
PhylomeDB	Q756B7.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ASHGO

Accession

Primary (citable) accession number: Q756B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program