Reviewed, UniProtKB/Swiss-Prot Q755F7 (CET1_ASHGO)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme subunit beta
    EC=3.1.3.33
Alternative name(s):
    Polynucleotide 5'-triphosphatase
    mRNA 5'-triphosphatase
      Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	AFL134W

Organism

Ashbya gossypii (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

33169 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mRNA cap methyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	mRNA-capping enzyme subunit beta		PRO_0000210111

Sequences

	Sequence		Length	Mass (Da)
	Q755F7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1D734613FA2401FC	FASTA	478	53,999
	10 20 30 40 50 60 MSSEKQEGSP RRVLSLSDLV NREDSKDASG NTAGAALKPL SNDEVRRRLA HEDYSSNTVS 70 80 90 100 110 120 QVDDETDTDD GLGEVAFDRA DFRFDFEGHQ RSGGRASGEA EAESGGPGHE KATQQPSDAI 130 140 150 160 170 180 ATSPDRESTE PRAMDIFEER ASLESKKNNL RKDLRVLNEI ASTARPGRYK VAPIWAQKWK 190 200 210 220 230 240 PTVRALQNVN SKDLMKIDVS FTQVIPDDDL TKSVQDWVYA TLLSIPPEQR QYVEVEMKFG 250 260 270 280 290 300 ILMDRSSDSQ RVTPPVSSQT VYMEADARMK PDVDERVFVE LNRYVKGISE LTENTGKFNI 310 320 330 340 350 360 IESHNKDEMY RAGINTQRPR FLRMSKDVKT GRVGEFIEKR RISQLLLFSP KDSYDVKISI 370 380 390 400 410 420 NVELPVPEND PPEKYMGQAP LNSRTKERIS YIHNDSCTRI DITKVTNHNK GKRDDAEVTH 430 440 450 460 470 EIELELNSQA LLAAFDKIAQ DSKDYATIVR TFLNNGTIIR RKLTSLSYEI FEGGKKVV

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / NRRL Y-1056 / CBS 109.51.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016819 Genomic DNA. Translation: AAS53240.1.
RefSeq	NP_985416.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q755F7.
Genome annotation databases
GeneID	4621643.
GenomeReviews	Gene locus AFL134W in contig AE016819_GR.
KEGG	ago:AGOS_AFL134W.
NMPDR	fig|33169.1.peg.3061.
Organism-specific databases
AGD	AFL134W.
Phylogenomic databases
OMA	VELEINT.
Enzyme and pathway databases
BRENDA	3.1.3.33. 279361.
Family and domain databases
InterPro	IPR004206. mRNA_capping_enz_bsu. [Graphical view]
Gene3D	G3DSA:3.20.100.10. mRNA_capping_enz_bsu. 1 hit.
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CET1_ASHGO

Accession

Primary (citable) accession number: Q755F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)