Q755D0 (MCE1_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA-capping enzyme subunit alpha

Alternative name(s):
GTP--RNA guanylyltransferase
Short name=GTase
mRNA guanylyltransferase
EC=2.7.7.50

Gene names

Name:	CEG1
Ordered Locus Names:	AFL107W

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Complete proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the eukaryotic GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mRNA cap methyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	mRNA-capping enzyme subunit alpha		PRO_0000210098
Sites
	Active site	66	1	N6-GMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q755D0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9CE70B191A391159	FASTA	463	53,647
	10 20 30 40 50 60 MDSRDAPEVP GIRQPANVTT DLKMIICKLL NSPKPAKTFP GSQPVSFQHV DIEEKLLAQD 70 80 90 100 110 120 YYVCEKTDGL RALMLIIMNP VTKEQGCFLI DRENNYYLLN GFRFPRLPRA NRKELLETLQ 130 140 150 160 170 180 DGTLVDGELV VQTNPATRLR ELRYLMFDCL AINGRALVQS PTSSRLAHLG KEFYKPYYDL 190 200 210 220 230 240 RAYYPDRCAT FPFKLSMKHM NFSFDLDRVA GSLDKLPHVS DGLIFTAVDT PYTVGGKDST 250 260 270 280 290 300 LLKWKPEQEN TVDFKMILEI PVVEDDTLPK KDRNRFYYNF DVKPSFHLYI WQGGPDVNTR 310 320 330 340 350 360 LHDFEQPFSK KELEILHRTY KVFAELQISD EQWAKMKALE QPLNGRIVEC AKDQETGEWK 370 380 390 400 410 420 FLRFRDDKLN GNHVSVVQKV LESISDSVKL EDLEHMIPRI KARWEERKSK KRTHSSISGP 430 440 450 460 MLPPVAETKA PREATQSRYI DDEDWSDEAD DDDEDSLKRA RIE

References

[1]

"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed: 15001715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016819 Genomic DNA. Translation: AAS53267.1.
RefSeq	NP_985443.1. NM_210797.1.
3D structure databases
ProteinModelPortal	Q755D0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q755D0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4621670.
GenomeReviews	Gene locus AFL107W in contig AE016819_GR.
KEGG	ago:AGOS_AFL107W.
NMPDR	fig|33169.1.peg.3088.
Organism-specific databases
AGD	AFL107W.
Phylogenomic databases
eggNOG	fuNOG04646.
HOGENOM	HBG330574.
OMA	GSQPVSF.
OrthoDB	EOG4RV611.
PhylomeDB	Q755D0.
Family and domain databases
InterPro	IPR001339. mRNA_cap_enzyme. IPR013846. mRNA_cap_enzyme_C. IPR017075. mRNA_capping_enz_asu. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K00987.
Pfam	PF03919. mRNA_cap_C. 1 hit. PF01331. mRNA_cap_enzyme. 1 hit. [Graphical view]
PIRSF	PIRSF036959. mRNA_cap_alpha. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCE1_ASHGO

Accession

Primary (citable) accession number: Q755D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program